RAB27B Mouse mAb - Anticorpo primario, specific to RAB27B, Mouse IgG1

Cat. No.: Ab124166

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG1

Clone Number

1596CT245.254.49

Conjugation

Unconjugated

Panoramica

Mouse anti Human RAB27B Antibody, Monoclonal (1596CT245.254.49), could be used for WB, Flow and so on.
Application:
WB: 1/2000
Flow: 1/25
Protein Function:
May be involved in targeting uroplakins to urothelial apical membranes.

Specifications

Product Name

RAB27B Mouse mAb - Anticorpo primario, specific to RAB27B, Mouse IgG1

Sinonimi

C25KG antibody | Rab27b antibody | RAB27B member RAS oncogene family antibody | RAS associated protein RAB27B antibody | Ras related protein Rab 27b antibody | Ras related protein Rab27b antibody | Ras-related protein Rab-27B antibody | RB27B_HUMAN antibo

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Specie ospite

Mouse

Specificità

RAB27B

Immunogeno

Recombinant protein of human RAB27B (AA 1-218)

Controllo positivo

WB: A431 and C6 cell lysates. Flow: A431 cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: RAB27B Mouse mAb (Ab124166)

RAB27B Mouse mAb (Ab124166) - Western Blot
All lanes: RAB27B Mouse mAb (Ab124166) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.0 s

Flow Cytometry: RAB27B Mouse mAb (Ab124166)

RAB27B Mouse mAb (Ab124166) - Flow Cytometry
Overlay histogram showing A431 cells stained with RAB27B Mouse mAb (Ab124166) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by RAB27B Mouse mAb (Ab124166) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  1/25;

• WB:

  1/2000;

Obiettivi associati (umani)

RAB27B Tbio Ras-related protein Rab-27B (0 Activities)

Discover Target: RAB27B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RAB27B Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.