GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)0.5 mg/mL
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Immagini
Recombinant BrdU Antibody (Ab210602) - Flow Cytometry Dot plot showing BrdU-treated HeLa cells stained with Recombinant BrdU Antibody (Ab210602) at 0.3 μg/mL for 1 hour at room temperature. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature. The Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used as the secondary antibody. Propidium Iodide was used to stain total DNA. Left - Unlabeled sample.
Recombinant BrdU Antibody (Ab210602) - IF/ICC IF analysis of Recombinant BrdU Antibody (Green) in HeLa cells. The cells were fixed with ethanol for 15 minutes and subjected to acid hydrolysis using 2M HCl for 15 minutes at room temperature to denature the DNA. They were then stained Recombinant BrdU Antibody (Ab210602) at 1.0 μg/mL in blocking buffer overnight at 4℃ and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Applicazione
Applicazione
Dilution info
IF/ICC
1.0 μg/mL
Flow Cytometry
0.01 μg/mL- 2.0 μg/mL
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
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Recensioni
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Application Protocols
Item-specific tested applications, recommended working dilutions, sample preparation, and positive controls are not specified for this item; refer to the product Spec Sheet/CoA and SDS for authoritative instructions.
General notes (for planning only; not item-specific directions):
Common applications for BrdU antibodies include IF/ICC, IHC, flow cytometry, and ELISA/slot blot following DNA denaturation. Optimize denaturation, blocking, and incubation parameters per sample type.
Prepare appropriate controls: no-BrdU samples, isotype controls (if known), and detection-only controls to assess background.
Defer to the manufacturer’s validated protocols once available for this exact SKU (Ab210602).
BrdU (5-bromo-2′-deoxyuridine) is a thymidine analog that becomes incorporated into DNA during the S phase via the nucleotide salvage and DNA replication pathways. It substitutes for thymidine triphosphate (dTTP) as BrdUTP during polymerization.
Incorporated BrdU serves as a haptenic marker enabling immunodetection of newly synthesized DNA, thereby reporting on cell proliferation, DNA repair synthesis, and replication dynamics.
Because BrdU is not a native nucleotide, antibodies can selectively recognize the brominated uracil moiety once DNA is denatured to expose bases.
Role of anti-BrdU antibodies (literature/general):
Enable cell cycle analyses (pulse-chase, cumulative labeling), S-phase fraction quantification, and mapping of proliferative zones in tissues.
Applied in combination with DNA content dyes (e.g., propidium iodide, DAPI) for bivariate flow cytometry of cell cycle phases.
In DNA repair studies, BrdU incorporation during repair synthesis highlights sites of active repair.
Item-specific biological attributes (if any):
Species reactivity, epitope mapping, cross-reactivity to related analogs (e.g., IdU, CldU), clonality, and isotype are not specified for this item; consult the Spec Sheet for definitive performance characteristics.
Buffer Applications
Formulation: “PBS Only” (per Product Data). Exact pH and ionic strength are not specified.
Practical buffer guidance (general; optimize per assay):
Working buffer: PBS or TBS with 0.05–0.1% Tween-20 for washes can reduce nonspecific binding in IF/IHC/ELISA workflows. For flow cytometry, PBS with 0.5–1% BSA and 0.05% sodium azide is common; however, this item is supplied azide-free—add preservatives only if compatible and validated in your lab.
Blocking: Use serum-free blockers (e.g., protein-based blockers or polymeric blockers) to maintain animal-free workflows. Avoid adding carrier proteins to the stock if you wish to retain “carrier-free” status; instead, add blockers only to working solutions.
DNA denaturation: For BrdU exposure in fixed samples, acid (HCl), heat, or DNase I buffers are used; buffer capacity and pH control are critical to preserve morphology while exposing the hapten.
Conjugation: Carrier-free PBS is advantageous for direct conjugation (e.g., NHS-dye to lysines) but PBS contains primary amines? Note: PBS does not contain primary amines and is generally compatible with NHS chemistry (general). If buffer exchange is needed (e.g., for click-ready dyes), perform rapid desalting.
Item-specific buffer recipes, recommended working dilutions, and validated formulations are not specified for this item; consult the Spec Sheet.
Green Alternatives
Sustainability considerations for antibody-based assays focus on buffer systems, preservatives, and workflow design rather than solvent substitution.
What this product already offers:
Animal Free recombinant production reduces reliance on animal-derived materials.
Azide Free avoids sodium azide, a toxic preservative with aquatic hazards, improving downstream waste profile (while requiring stricter asepsis).
Carrier Free / PBS Only simplifies formulations and can reduce additive burden.
Greener practice suggestions (general):
Use phosphate-buffered systems prepared with ultrapure water and minimize single-use plastics by adopting reusable, properly sterilized staining chambers where feasible.
Adopt room-temperature incubations when compatible to reduce cold-room energy use; validate performance.
Transition to enzyme-free DNA denaturation (e.g., controlled heat) where possible to minimize strong acid usage; balance with data quality requirements.
Implement micro-volume formats (e.g., 96-well, chamber slides) to reduce reagent consumption and waste.
Comparison of preservative strategies (general; not item-specific):
No preservative (this item): lowest toxicity footprint, highest aseptic demands.
Sodium azide: strong antimicrobial, but toxic to aquatic life and incompatible with HRP.
Alternative biocides (e.g., ProClin): effective at low ppm, but still hazardous waste; check compatibility.
Always validate modified “green” protocols against standard conditions to ensure data integrity.
Pharmaceutical Uses
This product is supplied strictly for research use only and is not intended for human or veterinary diagnostic or therapeutic applications.
Formulation/manufacturing context (general):
Recombinant, animal-free, carrier-free antibodies can be used as analytical reagents in process development laboratories for monitoring BrdU incorporation in cell-based assays, or as controls in method development. Such uses remain non-clinical.
The azide-free formulation is compatible with peroxidase-based detection systems and avoids azide-associated hazards in pharmaceutical facilities; however, absence of a preservative requires aseptic handling.
No pharmacopeial monograph is applicable to anti-BrdU antibodies, and no GMP status is indicated.
Item-specific regulatory or QC attributes:
Purity, endotoxin levels, bioburden, residual host-cell proteins, and documentation for regulated environments: Not specified for this item; refer to CoA/Spec Sheet.
Do not use in finished pharmaceutical products, diagnostic kits, or clinical protocols unless independently qualified and authorized under the appropriate regulatory framework.
Physical Properties
Item-specific physicochemical specifications are not provided for this recombinant antibody.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Concentration: Not specified for this item; refer to CoA/Spec Sheet.
Extinction coefficient (A280) and E1%: Not specified for this item; refer to CoA/Spec Sheet.
pH and buffer composition: Labeled “PBS Only”; exact pH and any stabilizers/cryoprotectants are not specified for this item.
Osmolality, conductivity, preservative level: Not specified for this item; refer to CoA/Spec Sheet.
General/literature properties (for context; not item specifications):
Typical IgG isoelectric point (pI): ~7–9 (literature; depends on isotype/sequence)
Protein stability is enhanced by minimizing freeze–thaw cycles and storing at ≤ −20 °C (literature), aligning with the item’s storage guidance.
Handling considerations (practical):
Viscosity and density are dominated by the aqueous PBS matrix (similar to water at laboratory temperatures; literature).
Solubility: Recombinant antibodies are water-soluble in physiological buffers (literature). Avoid mixing with strong denaturants unless required by assay protocols (e.g., DNA denaturation for BrdU detection is performed on fixed samples, not on the antibody stock).
Quality and Grades
Item bears the following quality/feature designations (per Product Data). Explanations are provided for clarity; where performance claims are implied, defer to CoA/Spec Sheet.
Recombinant: Produced in a controlled expression system, improving lot-to-lot consistency compared with hybridoma-derived monoclonals or serum-derived polyclonals.
Animal Free: Manufactured without animal-derived components during production, supporting workflows that avoid adventitious animal materials.
Carrier Free: No carrier proteins (e.g., BSA) added. Advantages: clean conjugation chemistry, accurate protein quantitation; Considerations: potentially lower stability without protein protectants.
Azide Free: No sodium azide preservative. Advantages: compatibility with peroxidase-based assays and live-cell–adjacent environments; Considerations: increased need for sterile technique and cold-chain maintenance.
PBS Only: Formulated solely in phosphate-buffered saline; exact pH, ionic strength, and any cryoprotectants not specified for this item.
ExactAb™ (brand/technology designation): Indicates defined recombinant sequence/format and performance targeting high lot reproducibility (general interpretation; refer to vendor documentation for specifics). Item-specific sequence/isotype not specified.
Validated / High Performance: Indicates internal application validation. Specific tested applications, species reactivity, and data are not specified for this item; consult the Spec Sheet for details.
Batch documentation:
Purity, concentration, residual host-cell proteins/DNA, endotoxin, and sterility/bioburden: Not specified for this item; refer to CoA/Spec Sheet.
Reaction and Applications
As an antibody reagent, this product is employed in immunochemical detection rather than chemical synthesis.
Typical research applications for BrdU antibodies (literature/general guidance; item-specific validations are not provided here):
Immunofluorescence (IF) / Immunocytochemistry (ICC): Detection of DNA-incorporated BrdU in cultured cells following a labeling pulse and fixation. Requires DNA denaturation (e.g., HCl, DNase) to expose BrdU.
Immunohistochemistry (IHC): Proliferation mapping in tissue sections after BrdU administration and fixation, with antigen retrieval/denaturation to unmask BrdU.
Flow cytometry (FCM/FACS): Quantification of S-phase populations after BrdU pulse, with intracellular staining following permeabilization and DNA denaturation.
ELISA or slot blot: Semi-quantitative detection of BrdU-labeled DNA.
Key practical notes (general):
DNA denaturation strategy: Acid (e.g., HCl), heat, or enzymatic (DNase I) methods can be used; excessive denaturation damages morphology and reduces antigenicity. Optimize for your sample type.
Controls: Include no-BrdU negative controls and isotype controls (if isotype known) to assess specificity and background.
Compatibility: Azide-free formulation is compatible with HRP-based detection systems. Carrier-free enables clean conjugation to fluorophores or enzymes if desired.
Item-specific tested applications, recommended dilutions, and species reactivity are not specified for this item; consult the Spec Sheet for validated use-cases.
Reaction Conditions
As an antibody reagent, there are no chemical “reaction” conditions in the synthetic sense. However, BrdU immunodetection requires DNA denaturation and antibody–antigen binding steps. The following are general, literature-based ranges for method development (not item-specific recommendations):
DNA denaturation: Acidic (e.g., 1–4 N HCl for minutes to tens of minutes), heat-mediated (e.g., citrate buffer near boiling), or enzymatic (DNase I) approaches. Optimize to balance epitope exposure and morphology.
Blocking: 15–60 min in protein blocker at RT to reduce nonspecific binding.
Primary antibody incubation: 1 h at RT or overnight at 4 °C (literature typical). Exact dilution and time for this item are not specified; determine empirically.
Detection: Fluorophore-conjugated secondary antibodies for IF/FC; HRP/AP systems for IHC/ELISA.
Washes: PBS or TBS with 0.05–0.1% Tween-20 between steps.
Environmental controls:
Protect fluorophores from light. Maintain cold chain for the stock solution; perform incubations at consistent temperatures to ensure reproducibility.
Item-specific optimal conditions, incubation times, and dilutions are not specified for this item; consult the Spec Sheet.
Safety and Handling
GHS classification, signal word, H-statements, and pictograms: Not specified for this item; refer to the product SDS for authoritative safety information.
Nature of product: Recombinant protein antibody in PBS; labeled azide-free (no sodium azide preservative).
PPE and general lab practices (good laboratory practice; consult SDS):
Wear lab coat, gloves, and safety glasses when handling.
Avoid aerosol generation and ingestion; do not pipette by mouth.
Work on clean, dedicated surfaces to prevent contamination of biological assays.
Storage and stability:
Store at −20 °C. Avoid repeated freezing and thawing (per Product Data). Aliquot upon first use to minimize freeze–thaw cycles.
Do not mix with oxidants, proteases, or extreme pH solutions that can denature proteins.
For BrdU immunodetection, DNA denaturation steps (e.g., HCl) are performed on fixed samples; keep the antibody stock away from strong acids/bases.
First aid (overview; defer to SDS):
Skin/eye contact: Rinse with water for several minutes. Remove contaminated clothing.
Inhalation: Move to fresh air.
Ingestion: Rinse mouth; seek medical attention as needed.
Waste: Dispose of proteinaceous solutions and consumables according to institutional biosafety and chemical hygiene policies.
Solvent Selection
This is an aqueous protein reagent formulated as “PBS Only” and is not intended for use in organic solvents.
Primary medium: Phosphate-buffered saline (PBS). Exact pH and composition are not specified for this item.
Miscibility: Ready-to-use in water-based systems. Avoid mixing with strong acids/bases or organic solvents that denature proteins (e.g., >20–30% methanol, acetone) unless part of a validated protocol.
Additives: Carrier-free and azide-free; if long-term storage is needed, end users sometimes add sterile glycerol to 40–50% (v/v) at −20 °C (general practice, literature). This is not part of the supplied item; validate for your assay.
When to choose this reagent:
Preferable for immunodetection of incorporated BrdU in fixed cells/tissues where azide-free, carrier-free formulation benefits downstream conjugation or sensitive peroxidase-based detection.
Alternative media and considerations (general):
TBS vs PBS: Some epitopes or detection systems prefer Tris-buffered saline; if buffer exchange is needed, perform gentle desalting and re-validate binding.
Blocking/stabilizing additives (e.g., BSA, casein, trehalose) can improve robustness in assays but would negate “carrier-free” status; add only if required and keep separate from the master stock.
Storage and Reconstitution
Storage condition (item-specific): Store at −20 °C. Avoid repeated freezing and thawing.
Shipping (item-specific): Ice chest + ice pads. Inspect upon receipt and return to recommended storage promptly.
Appearance and physical state: Not specified for this item; refer to CoA/Spec Sheet.
Concentration and volume: Not specified for this item; refer to CoA/Spec Sheet.
Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied lyophilized, follow the label for diluent type and target concentration; if supplied as liquid, mix gently by inversion—do not vortex vigorously.
Good practices (general):
Upon first thaw, aliquot into single-use volumes to avoid freeze–thaw damage.
Store working aliquots at 4 °C for short-term use (days) if sterility can be maintained; return long-term stocks to −20 °C promptly.
Do not add preservatives or carriers to the master stock unless validated for your application; maintain the “carrier-free” status for conjugation workflows.
Any additional stabilizers, exact buffer composition (beyond “PBS Only”), and shelf life are not specified for this item; consult the product documentation.
Intended antigen: BrdU (5-bromo-2′-deoxyuridine) incorporated into DNA
Molecular formula: Not applicable to antibody proteins; item-specific value not provided.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Typical IgG MW ~150 kDa (literature; for context only)
SMILES / InChI / InChIKey: Not applicable to protein biologics; not provided.
Protein class: Recombinant antibody; azide-free, carrier-free, animal-free (per Product Data). Isotype, clonality, and clone ID are not specified for this item.
Structural description (general, literature):
Antibodies are Y‑shaped immunoglobulin proteins composed of two heavy and two light chains with variable domains (VH/VL) forming the antigen-binding sites (paratopes). The BrdU antibody recognizes the brominated uracil nucleobase within DNA after BrdU incorporation, typically requiring DNA denaturation to expose the hapten.
Notes on identity and formulation (item-specific where provided):
Formulation: “PBS Only” indicated; no carrier proteins (e.g., BSA) and no sodium azide (azide-free). Additional excipients, concentration, and exact buffer composition are not specified for this item.
Synthetic Utility
Not typically applicable. As an immunoreagent, this product is used for detection of BrdU-labeled DNA in biological samples rather than for chemical synthesis or as a building block.
For users planning conjugation (general guidance):
The carrier-free, PBS-only formulation is suitable for direct chemical modification (e.g., NHS-esters to lysines, maleimides to engineered cysteines) when buffer components are compatible with the chosen chemistry.
For reactions incompatible with phosphate or requiring low ionic strength, perform a rapid buffer exchange (e.g., into 50 mM sodium bicarbonate, pH 8.3, for NHS labeling) using desalting columns, then re-qualify antigen binding post-conjugation.
Item-specific functional groups, degree of labeling (DOL), and conjugation recommendations are not specified for this item and should be established empirically.
Target Specificity
Intended antigen: BrdU (5-bromo-2′-deoxyuridine) incorporated into DNA.
Epitope: Not specified for this item; refer to CoA/Spec Sheet.
Clone ID / clonality / isotype / host: Not specified for this item; refer to CoA/Spec Sheet.
Species reactivity: Not specified for this item; refer to CoA/Spec Sheet. BrdU is a synthetic nucleoside incorporated across many species’ DNA, but antibody accessibility depends on sample preparation (general note).
Cross-reactivity: Not specified for this item; consult the Spec Sheet. Some anti-BrdU clones in the literature exhibit varying cross-reactivity with halogenated analogs (e.g., IdU, CldU); verify for your workflow.
Validation status and data: Labeled “Validated, High Performance” in Product Data; specific datasets, titrations, and application images are not included here. Please consult the product documentation for definitive specificity and performance information.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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