Recombinant CD25 Antibody - Anticorpo primario, specific to Mouse CD25

Cat. No.: Ab222912
Disponibile su ordine
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
ELISA, Flow Cytometry
Host species
consiglio
Reactive species
Mouse
Isotype
Rat IgG1
Clone Number
P18B24
Conjugation
Unconjugated
★
Size
Germania (EU)
USA*
Price
Qty
10μg
Ab222912-10μg
Su ordinazione · 8–12 settimane
34,62€
50μg
Ab222912-50μg
Su ordinazione · 8–12 settimane
69,33€
100μg
Ab222912-100μg
Su ordinazione · 8–12 settimane
104,04€
1mg
Ab222912-1mg
Su ordinazione · 8–12 settimane
242,88€
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Panoramica

Recombinant CD25 Antibody, clone P18B24, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name
Recombinant CD25 Antibody - Anticorpo primario, specific to Mouse CD25
Sinonimi
Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor subunit alpha antibody | IL-2-RA antibody | IL-2R subunit alpha antibody | IL2 RA antibody | IL2 R
Specifiche e purezza
Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA
Meccanismi biochimici e fisiologici
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Specie ospite
consiglio
Specificità
Mouse CD25
Immunogeno
IL-2-dependent cytolytic mouse T-cell clone B6.1
Controllo positivo
Flow Cytometry: 3-day ConA activated C57BL/6 splenocytes
Coniugazione
Unconjugated
Grado
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated
Tipo di catena leggera
lambda
SDS-PAGE
150 kDa
Metodo di purificazione
Protein A purified
Concentrazione
See COA
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini

Recombinant CD25 Antibody (Ab222912) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day Con A-stimulated (right) C57BL/6 mouse splenocytes labelling CD25 (red) with Recombinant CD25 Antibody (Ab222912) at 0.2 μg/mL for 1 hour at 4°C and detected with AF 647 - conjugated affinipure goat anti - Rat IgG. Blue - Rat IgG Isotype Control Antibody (Ab141774). Black - Unlabelled control, cells without incubation with primary antibody.

Recombinant CD25 Antibody (Ab222912) - SEC
The purity of Recombinant CD25 Antibody (Ab222912) is more than 95% verified by HPLC.

Applicazione
ApplicazioneDilution info
Flow Cytometry

≤0.5 µg per million cells in 100 µl volume

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Obiettivi associati (umani)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

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