QUALITÀ & PUREZZARecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.5 μL/test
ExactAb™, Validated, Recombinant, 5 μL/test ExactAb™,Recombinant,Validated per flussi di lavoro cromatografici e analitici sensibili che richiedono un'interferenza minima della linea di base.
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Stoccaggio e spedizione
Store at 2-8°C,Protected from light,Do not freeze Spedizione Wet ice,Do not freeze. Consultare il COA specifico del lotto per le specifiche esatte.
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Documenti di qualità
SDS, COA, scheda tecnica e scheda delle specifiche disponibili per il download. COA specifico del lotto accessibile tramite ricerca per numero di lotto.
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Prova documentale
Citato in 0 pubblicazioni peer-reviewed su cromatografia, sintesi organica e reazioni di accoppiamento incrociato.
Panoramica
Recombinant CD3ε Antibody, clone P93B35, could be used for Flow Cytometry.
Specifiche
Product Name
Recombinant CD3 Antibody (AF488) - Anticorpo primario, specific to Human CD3ε
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Specie ospite
Mouse
Specificità
Human CD3ε
Immunogeno
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Controllo positivo
Flow Cytometry: Human peripheral blood lymphocytes
Coniugazione
AF488
Eccitazione (nm)
Blue Laser(488nm)
Emissione (nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grado
ExactAb™, Recombinant, Validated
Tipo di catena leggera
kappa
Metodo di purificazione
Protein A purified
Concentrazione
5 μL/test
Product Line
Prodotti biologici
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C,Protected from light,Do not freeze
Spedito in
Wet ice,Do not freeze
Stabilità e conservazione
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Immagini
Recombinant CD3 Antibody (AF488) (Ab223447) - Flow Cytometry Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (AF488) (Ab223447) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
Recombinant CD3 Antibody (AF488) (Ab223447) - Flow Cytometry Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (AF488) (Ab223447) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Applicazione
Applicazione
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentazione
📋 Scheda di dati di sicurezza (SDS)
Documento completo su pericoli, manipolazione, stoccaggio e conformità normativa.
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Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD3 Antibody (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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