Recombinant CD3 Antibody - Primary antibody, specific to CD3, Mouse IgG2a

CAS: 140608-64-6 Cat. No.: Ab179443
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE&SEC-HPLC) See COA
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2a
Clone Number
OKT3
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab179443-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$59.90
1mg
Ab179443-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$259.90
5mg
Ab179443-5mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$739.90
Enter a quantity for the sizes you want to add.
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Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&SEC-HPLC),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application:
Flow: ≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name
Recombinant CD3 Antibody - Primary antibody, specific to CD3, Mouse IgG2a
Synonyms
CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&SEC-HPLC), See COA
Host species
Mouse
Specificity
CD3
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, PBS Only, Validated
Isotype
Mouse IgG2a
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
See COA
CAS
140608-64-6
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Images

Recombinant CD3 Antibody (Ab179443) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab179443) (right panel) conjugated with biotin compared with Human IgG (Ab170213) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Recombinant CD3 Antibody (Ab179443) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab179443) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, human IgG (Ab170213) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0825631Certificate of AnalysisJun 22, 2026 Ab179443
ZJ25F0825632Certificate of AnalysisJun 22, 2026 Ab179443
ZJ25F0825633Certificate of AnalysisJun 22, 2026 Ab179443
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View PBS Only grade guide →

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