Recombinant Human EphB4 Protein

Cat. No.: rp184032

QUALITÀ & PUREZZA Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Accession #

P54760

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Panoramica

Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor tyrosine kinase family and binds Ephrin-B2. The A and B class Eph proteins have a common structural organization. The human EphB4 cDNA encodes a 987 amino acid precursor that includes a 15 amino acid (aa) signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 427 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM). Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. The ECD of human EphB4 shares 89% aa sequence identity with mouse EphB4 and 42 - 45% aa sequence identity with human EphB1, 2, and 3. EphB4 is expressed preferentially on venous endothelial cells (EC) and inhibits cell-cell adhesion, chemotaxis, and angiogenesis. Opposing effects are induced by signaling through Ephrin-B2 expressed on arterial EC: adhesion, endothelial cell migration, and vessel sprouting. EphB4 signaling contributes to new vascularization by guiding venous EC away from Ephrin-B2 expressing EC. Ephrin-B2 signaling induces arterial EC to migrate towards nascent EphB4 expressing vessels. The combination of forward signaling through EphB4 and reverse signaling through Ephrin-B2 promotes in vivo mammary tumor growth and tumor-associated angiogenesis. EphB4 promotes the differentiation of megakaryocytic and erythroid progenitors but not granulocytic or monocytic progenitors.

Specifiche

Product Name

Recombinant Human EphB4 Protein

Sinonimi

soluble EPHB4 variant 2 | soluble EPHB4 variant 3 | Tyro11 | Tyrosine-protein kinase receptor HTK | Tyrosine-protein kinase TYRO11 | EC 2.7.10 | EC 2.7.10.1 | EPH receptor B4 | EphB4 | ephrin type-B receptor 4 | hepatoma transmembrane kinase | Htk | HTKep

Grado

Animal Free, Carrier Free, His Tag, PBS Only

Specifiche e purezza

Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Bioattività

Testing in progress

Concentrazione di endotossina

<1.0 EU/μg

Sistema di espressione

HEK293

Aminoacidi

1-539 aa

Sequenza

MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLAHHHHHH

Tag proteico

C-His

Numero d'ordine

P54760

Peso molecolare previsto

57.9 kDa

SDS-PAGE

59.4-77.7 kDa, under reducing conditions; 52.4-65.0 kDa, under non-reducing conditions

Tipo di molecola

Proteine

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Lyophilized

Ricostituzione

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Immagini

SDS-PAGE: Recombinant Human EphB4 Protein (rp184032)

Recombinant Human EphB4 Protein (rp184032) - SDS-PAGE
3 μg/lane of Recombinant Human EphB4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 59.4-77.7 kDa under reducing conditions and 52.4-65.0 kDa under non-reducing conditions.

Binding Activity​: Recombinant Human EphB4 Protein (rp184032)

Recombinant Human EphB4 Protein (rp184032) - Binding Activity​ 

Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.