α-Rhamnosidase, CAS No.37288-35-0

CAS: 37288-35-0 Cat. No.: R1438570

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥1500 U/mL; ~110 U/mg protein

Accession #

Q82PP4

Bioactivity

≥1500 U/mL; ~110 U/mg protein

Panoramica

High purity recombinant α-Rhamnosidase (prokaryote) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: Hydrolysis of terminal non-reducing α-L-rhamnose residues in α-L-rhamnosides.

Application examples: Applications in carbohydrate and biofuels research.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 75,400) 

- One major bands on isoelectric focusing (pI ~ 5.9) 

2. PHYSICOCHEMICAL PROPERTIES: 

pH Optima: 6.0-6.5 pH 

Stability: 4.0-9.0 (> 75% control activity after 24 h at 4°C) 

Temperature Optima: 50°C (10 min. reaction) 

Temperature Stability: up to 50°C (> 90% control activity after 15 min.) 

3. STORAGE CONDITIONS: The enzyme is supplied as an ammonium sulphate suspension in 0.02% (w/v) sodium azide and should be stored at 4°C. For assay, this enzyme should be diluted in sodium phosphate buffer (20 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.

Specifications

Product Name

α-Rhamnosidase, CAS No.37288-35-0

Sinonimi

alpha-L-rhamnosidasealph | a-L-rhamnoside rhamnohydrolase

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifiche e purezza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥1500 U/mL; ~110 U/mg protein

Bioattività

≥1500 U/mL; ~110 U/mg protein

Numero d'ordine

Q82PP4

Peso molecolare previsto

75.4 kDa

CAS

37288-35-0

Numero della Commissione Enzimi

EC 3.2.1.40

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥1500 U/mL; ~110 U/mg protein

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definizione dell'unità

One Unit of α-L-rhamnosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-rhamnoside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 50°C.

Domande frequenti

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 37288-35-0.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.