Ribosomal Phosphoprotein P2

Cat. No.: R572848

GRADE & PURITY ≥80%(SDS-PAGE)

Panoramica

Biochemical Test:SDS-PAGE (purity> 80%); Western blot with i: anti-P2 autoantibody positive sample; ii: monoclonal anti His-tag antibody.

Calculated Isoelectric Point:pH 5.0

Specifications

Product Name

Ribosomal Phosphoprotein P2

Specifiche e purezza

≥80%(SDS-PAGE)

Fonte

Recombinant. Expressed in E. coli bacterial cells.

Peso molecolare previsto

13 kDa

Tipo di molecola

Proteine

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Application Protocols

Only item-specific, validated protocols should be listed here; however, none are provided in the Product Data for SKU R572848.

Item-specific details not provided: • Tested applications (e.g., WB, IHC, IF, ELISA, IP): Not specified for this item; refer to CoA/Spec Sheet. • Recommended working dilutions: Not specified for this item; refer to CoA/Spec Sheet. • Positive controls and antigen retrieval conditions: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature; to be adapted after consulting the CoA): • Include appropriate positive controls (cells/tissues with robust ribosomal protein expression) and negative controls (isotype control or antigen-blocked antibody) to validate specificity. • Optimize antibody concentration by checkerboard titration; verify linear dynamic range for ELISA or exposure times for WB/IF.

Biological Roles

Ribosomal Phosphoprotein P2 (RPLP2) is an acidic protein component of the eukaryotic 60S ribosomal stalk. The P-stalk, formed by P0 in complex with P1/P2 heterodimers, plays a pivotal role in translation elongation by recruiting and stimulating translational GTPases.

Key features (literature): • Structure–function: P2 contains an N-terminal dimerization domain that pairs with P1, and a conserved, highly acidic C-terminal tail that engages elongation factors (eEF1/eEF2) and other translational factors, facilitating GTP hydrolysis cycles on the ribosome. • Post-translational modification: P2 is a phosphoprotein; phosphorylation modulates interactions with P0, P1, and translation factors, potentially tuning elongation efficiency and stress responses. • Conservation and immunogenicity: The C-terminal epitope is conserved across species, making it a common antigenic determinant recognized by anti-P family antibodies in diverse organisms (context from literature). • Cellular localization: Predominantly cytosolic as part of 60S subunits; may be detected in polysomes and ribosome-associated fractions.

Applications informed by biology: • As an antigen, RPLP2 is used to generate and validate antibodies targeting the ribosomal P-stalk. • As a biomolecular marker, it assists in probing ribosome assembly, translation factor engagement, and ribosomal stress responses in cell biology.

Note: These biological roles are literature-based descriptions of the antigen and are not item-specific performance claims.

Buffer Applications

This section is not typically applicable. Antibodies and protein antigens are end-users of buffers rather than components for preparing classical buffer systems.

General notes (for handling): • Common working buffers include PBS or TBS with 0.05–0.1% Tween-20 for immunoassays, and blocking agents such as BSA or nonfat milk for western blotting. • For storage, stabilizers (e.g., 10–50% glycerol, 0.02–0.05% sodium azide) are sometimes included; verify presence and concentrations on the CoA/Spec Sheet.

Item-specific buffer composition and recommended dilutions for SKU R572848: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Green chemistry selection is not directly applicable to antibodies or protein antigens, which are handled in aqueous buffers, not organic solvent processes.

General sustainability considerations for protein reagents: • Minimize waste by aliquoting to avoid freeze–thaw cycles and subsequent discard of degraded material. • Use recyclable cold packs and insulated shippers when cold-chain is required. (Note: this item is listed as “Shipped In: Normal” per Product Data.) • Prefer phosphate-buffered saline and benign preservatives where feasible; avoid unnecessary addition of hazardous solvents or chaotropes.

No direct greener “substitute solvent” applies here.

Pharmaceutical Uses

Not applicable. This product is supplied for research use only and is not intended for human or veterinary use, formulation, or clinical manufacturing.

General context (non-clinical): Antibodies and recombinant proteins like RPLP2 are commonly employed in research assay development, target validation, and analytical controls in non-clinical laboratories.

Regulatory note: No pharmacopeial status, excipient role, or GMP manufacturing claims are provided for this SKU. For any regulated applications, obtain detailed documentation (CoA, TSE/BSE statements, adventitious agent testing) directly from the manufacturer.

Physical Properties

Item-specific physical specifications (concentration, formulation buffer, pH, protein/antibody content, excipients) are not provided in the Product Data.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. • Concentration: Not specified for this item; refer to CoA/Spec Sheet. • Formulation: Not specified for this item; refer to CoA/Spec Sheet. • pH/osmolality: Not specified for this item; refer to CoA/Spec Sheet. • Extinction coefficient: Not specified for this item; refer to CoA/Spec Sheet.

General/literature context for the antigen (RPLP2 protein): • Typical length and mass (literature): ~115–120 amino acids; calculated MW ~11–12 kDa (species-dependent and excluding tags/PTMs). • Isoelectric point (literature): Acidic character (pI commonly <6) due to high Asp/Glu content; exact pI depends on sequence and phosphorylation state. • Solubility (literature): Recombinant RPLP2 and antibodies are generally water-soluble in standard biological buffers (e.g., PBS) with stabilizers (e.g., BSA, glycerol) as needed.

Important: The above properties are generic literature values for the biological target and are not item-specific specifications. For exact properties and certificate data of SKU R572848, consult the CoA/Spec Sheet.

Quality and Grades

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

• What to expect for antibody/antigen-quality listings (general guidance):

• Antibodies are typically supplied as purified Ig (e.g., Protein A/G purified, affinity-purified) or as antiserum; recombinant proteins/antigens may be tag-fused and purified by affinity chromatography. The exact purification grade (and residuals such as endotoxin, host-cell proteins, imidazole, glycerol, BSA, sodium azide) should be confirmed on the CoA.
• Functional qualification often includes application validation (WB, IHC, ELISA, IF), species reactivity, and, for proteins, identity by MS/western blot and purity by SDS-PAGE. None of these are specified for this SKU in the provided data.

• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet. Presence of agents such as glycerol, trehalose, BSA, or sodium azide affects storage and handling.

• Batch documentation: For regulated or high-reproducibility workflows, request lot-specific CoA detailing concentration, formulation, and any performance qualifications.

In summary, consult the CoA/Spec Sheet for SKU R572848 for all item-specific grade and quality attributes; do not infer purity or validation status beyond what is documented.

Reaction and Applications

Not typically applicable in the synthetic-chemistry sense for antibodies or protein antigens. These reagents are not used as chemical solvents or substrates in classical organic reactions.

Relevant laboratory applications (general, literature): • Immunoassays: Anti–Ribosomal P2 antibodies are commonly applied in ELISA, western blot, immunofluorescence, and immunohistochemistry to detect RPLP2. If SKU R572848 is an antibody, application suitability and recommended dilutions must be confirmed on the CoA. • Protein standards/antigen controls: If the item is recombinant RPLP2, it can serve as a positive control antigen for assay development and antibody screening. • Epitope mapping and binding studies: The conserved acidic C-terminal tail of P2 is frequently used in peptide-based binding assays to characterize antibody specificity (literature context).

Item-specific tested applications, dilutions, and protocols: Not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

Classical reaction conditions (solvent, temperature, catalysts) are not applicable to antibodies or protein antigens.

Assay-condition considerations (general): • ELISA: Typical coating 0.5–2 µg/mL antigen in carbonate buffer (pH ~9.6), block with BSA or casein; primary antibody dilutions often range 1:1,000–1:10,000 depending on affinity and format. Incubate at room temperature or 37°C per protocol. • Western blot: Antigen load 10–30 µg total protein per lane; primary antibody dilutions vary widely (e.g., 1:500–1:5,000). Transfer and blocking conditions as per membrane chemistry. • IF/IHC: Antigen retrieval and fixation conditions depend on tissue/cell prep; antibody dilutions and incubation times are empirically optimized.

Important: The above are literature-based generalities. Item-specific recommended conditions for SKU R572848 (validated applications, suggested dilutions, incubation times) are not specified; consult the CoA/Spec Sheet.

Safety and Handling

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS for authoritative safety information.

• General laboratory safety for protein/antibody reagents:

• PPE: Wear lab coat, safety glasses, and appropriate gloves (e.g., nitrile). Avoid inhalation of aerosols and contact with skin/eyes.
• Handling: Work using aseptic technique to avoid contamination of biological reagents. Avoid repeated freeze–thaw unless specified to be stable.
• Incompatibilities: Avoid strong oxidizers and extreme pH unless validated for your protocol. Sodium azide (if present as a preservative) can form metal azides with copper/lead plumbing—flush with copious water; check the SDS/label for presence of azide.
• First aid (overview): In case of skin/eye contact, rinse with water for several minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth. Seek medical attention as appropriate. Defer to SDS.

• Biological safety: This product is for research use only. Not for human or animal therapeutic or diagnostic applications. Handle according to your institution’s biosafety practices.

• Waste: Dispose of unused material and contaminated consumables according to local regulations and your institution’s biosafety policy.

Always consult the product label and SDS for SKU-specific hazards and instructions.

Solvent Selection

This section is not typically applicable to antibodies or protein targets. These materials are usually supplied in aqueous buffers (e.g., PBS, Tris) rather than used as solutes in organic solvent systems.

Practical notes (general): • If the product is an antibody: Maintain in the supplied buffer; avoid high proportions of organic solvents that can denature proteins. For conjugation or lyophilization, exchange into the recommended buffer using desalting columns or dialysis. • If the product is a recombinant protein antigen: Follow buffer recommendations on the CoA/Spec Sheet. Additives like glycerol (10–50%) may improve stability at sub-zero storage; high salt or detergents can affect downstream assays.

Item-specific solvent/buffer composition for SKU R572848: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (item-specific): Room temperature (per Product Data). Note: Follow the label and CoA for definitive storage instructions. If the vial specifies a different temperature (e.g., 2–8°C or −20°C), prioritize the product label.

• Shipped in: Normal (per Product Data).

• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied lyophilized, reconstitute with sterile buffer (e.g., PBS) to the recommended concentration and allow the material to fully dissolve by gentle inversion—avoid vigorous vortexing.

• Aliquoting and stability (general guidance):

• To minimize degradation and activity loss, prepare single-use aliquots upon first use.
• Avoid repeated freeze–thaw cycles. If long-term storage at sub-zero temperatures is recommended on the CoA, consider adding cryoprotectants (e.g., 10–50% glycerol) if compatible with downstream assays.

• Light and contamination: Protect from microbial contamination; use sterile technique. Protect labeled conjugates (if any) from light.

Item-specific buffer composition, preservatives, concentration, and shelf life: Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

• Item type: Listed under antibodies (Category: 全部 / 可售 / 抗体). The product name suggests an antibody directed against, or material related to, the protein Ribosomal Phosphoprotein P2 (RPLP2). Item-specific identity parameters for an antibody (clone, isotype, host, antigen species) are not provided in the Product Data.

• Molecular formula (item-specific): Not specified for this item; refer to CoA/Spec Sheet.

• Molecular weight (item-specific): Not specified for this item; refer to CoA/Spec Sheet.

• SMILES, InChIKey: Not applicable to proteins/antibodies; not specified for this item.

• Structural features (general, literature):

• Ribosomal P2 is an acidic phosphoprotein of the large (60S) ribosomal subunit stalk, typically ~115–120 amino acids in eukaryotes and rich in acidic residues (Asp/Glu). It pairs with P1 and associates with P0 to form the P-stalk (P0–(P1/P2)2) critical for translation factor interactions.
• The C-terminal tail (conserved DDDMGFGLFD motif in many species; literature) mediates binding to elongation factors and is a key epitope in several anti-P antibodies.

• 2D/primary structure description (literature): Single polypeptide chain with an N-terminal helical domain that dimerizes (P1/P2 heterodimer), and a flexible acidic C-terminal tail that is often phosphorylated.

Note: The above structural notes describe the biological antigen (RPLP2) in general terms and are literature-based, not item-specific.

Synthetic Utility

Not applicable in the context of organic synthesis. Antibodies and protein antigens are not used as reagents for chemical bond-forming reactions or building blocks in small-molecule synthesis.

Research relevance (general): • As capture/detection reagents in immunoassays, antibodies to RPLP2 enable selective enrichment or detection of the P2 protein from biological samples. • Recombinant RPLP2 can serve as an affinity ligand or positive control for validating antibody specificity.

Item-specific functionalization (e.g., conjugation state, tags, labels): Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

This section is limited to item-specific data. For SKU R572848, the following attributes are not provided in the Product Data: • Antigen species/reactivity: Not specified for this item; refer to CoA/Spec Sheet. • Clone/host/isotype (if an antibody): Not specified for this item; refer to CoA/Spec Sheet. • Epitope mapping (linear/conformational): Not specified for this item; refer to CoA/Spec Sheet. • Cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.

General note (literature): Antibodies to ribosomal protein P2 frequently recognize the conserved acidic C-terminal epitope shared among P1/P2 family members; confirm exact specificity with peptide competition or recombinant protein controls.