RNASEH2A Mouse mAb - Anticorpo primario, specific to RNASEH2A

Cat. No.: Ab125320

GRADE & PURITY See COA

Application

WB

Host species

Mouse

Reactive species

Mouse, Umano

Isotype

Mouse IgG1

Clone Number

OTI7H10

Conjugation

Unconjugated

Panoramica

Mouse anti Human Ribonuclease H2，subunit A/RNASEH2A Antibody, Monoclonal (OTI7H10), could be used for WB and so on.

Specifications

Product Name

RNASEH2A Mouse mAb - Anticorpo primario, specific to RNASEH2A

Sinonimi

AGS4 | JUNB | RNHL | RNHIA | RNASEHI | AGS4 antibody | Aicardi Goutieres syndrome 4 protein antibody | Aicardi-Goutieres syndrome 4 protein antibody | EC 3.1.26.4 antibody | JUNB antibody | Ribonuclease H2 subunit A antibody | Ribonuclease HI large subuni

Specifiche e purezza

See COA

Meccanismi biochimici e fisiologici

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the e

Specie ospite

Mouse

Specificità

RNASEH2A

Immunogeno

Full length recombinant protein of human RNASEH2A produced in E.coli.

Controllo positivo

WB: HEK293T transfected with pCMV6-ENTRY RNASEH2A, Jurkat lysates.

Coniugazione

Unconjugated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/2000;

Obiettivi associati (umani)

RNASEH2A Tbio Ribonuclease H2 subunit A (0 Activities)

Discover Target: RNASEH2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item‑specific tested applications and dilutions

• Not specified for this item; refer to CoA/Spec Sheet.

General starting points (literature/practice; not item‑specific)

• Western blot (WB): Start 1:1,000–1:5,000 for primary; use PVDF/nitrocellulose, block with 5% BSA or milk; adjust based on signal and background. RNASEH2A expected molecular band size depends on species and isoform; confirm with literature for your system.
• Immunofluorescence (IF): Start 1:100–1:500; fix with 4% PFA, permeabilize with 0.1–0.3% Triton X‑100; counterstain with DAPI; include appropriate controls (secondary‑only, isotype).
• Immunohistochemistry (IHC‑P): Start 1:50–1:200; perform heat‑induced epitope retrieval (citrate pH 6.0 or Tris‑EDTA pH 9.0); block endogenous peroxidase if using HRP.
• Immunoprecipitation (IP): 1–5 μg antibody per mg total protein lysate; capture with Protein A/G resin compatible with the antibody isotype (isotype for this item not specified).
• ELISA: Coat antigen 0.5–2 μg/mL; primary 1:1,000–1:10,000 depending on format.

Controls and validation

• Include positive/negative biological controls, loading controls (WB), and secondary‑only controls (IF/IHC). Optimize empirically for cell line/tissue and detection system.

Biological Roles

Target background (literature/general knowledge)

• RNASEH2A is the catalytic subunit A of the RNase H2 complex (RNASEH2A/B/C), which cleaves the RNA strand of RNA:DNA hybrids and incises at single ribonucleotides embedded in genomic DNA. This initiates ribonucleotide excision repair, preventing genome instability.
• Functional pathways: DNA replication maturation (Okazaki fragment processing), resolution of R‑loops, maintenance of genome integrity, and coordination with DNA repair pathways including FEN1 and DNA ligase I (reported in literature).
• Subcellular localization: Predominantly nuclear; associated with replication foci during S‑phase (literature reports; may vary by cell type).
• Expression: Ubiquitous with enrichment in proliferative tissues; specific expression levels are cell‑context dependent (literature).

Relevance of an anti‑RNASEH2A mAb

• Enables detection/quantification of RNASEH2A protein levels, assessment of complex assembly (via co‑IP with RNASEH2B/C), and visualization of nuclear patterns by IF/IHC.

Item‑specific caveat

• Species reactivity, epitope, clone ID, and validation data for Ab125320 are not specified for this item; consult CoA/Spec Sheet for experimental planning.

Buffer Applications

Applicability

• Antibody performance is tightly linked to buffer composition; although the exact buffer for Ab125320 is not specified, the following general practices apply.

General buffer systems (literature/practice)

• Storage/stock: PBS, pH 7.2–7.4, optionally with 0.02–0.05% sodium azide and 10–50% glycerol for −20 °C storage to reduce freeze–thaw. For long‑term lyophilized stocks, sugars (trehalose/sucrose) can stabilize during drying and reconstitution.
• Working buffers: PBS or TBS with 0.05–0.1% Tween‑20 for washing; blocking with 3–5% BSA, casein, or milk (WB/ELISA) reduces nonspecific binding. For IF/IHC, serum from the host of the secondary antibody can be used for blocking.
• Antigen retrieval (IHC): Citrate buffer pH 6.0 or Tris‑EDTA pH 9.0 heat‑induced retrieval, optimized empirically for RNASEH2A epitope exposure (general guidance).
• Enzyme‑based detection: If using HRP, avoid azide in incubation buffers (azide inhibits peroxidase). For AP detection, maintain appropriate buffer pH (8.8–9.5) for activity.

Item‑specific note

• Buffer composition, presence of carrier proteins, and preservative content for Ab125320 are not specified; verify compatibility with your assay.

Green Alternatives

Context

• Green solvent alternatives are not directly relevant to antibody reagents. Environmental considerations focus instead on buffer selection, preservative handling, cold‑chain logistics, and waste minimization.

General sustainability practices for antibodies (literature/practice)

• Use concentrated stock solutions and aliquoting to reduce waste from freeze–thaw degradation.
• Prefer reusable cold packs and insulated shippers; consolidate orders to minimize shipping‑related emissions.
• Minimize azide‑containing waste; where permitted, collect separately and dispose in accordance with local regulations. Consider azide‑free formulations when compatible with your application (not specified for this item).
• Employ PBS/TBS made from high‑purity tablets or powders to reduce packaging footprint.

Item‑specific info

• No formulation details (e.g., presence/absence of azide, glycerol, or carrier protein) are provided for Ab125320; consult the CoA/SDS for environmentally pertinent information.

Pharmaceutical Uses

Not applicable to this product

• Ab125320 is supplied for research use only. No pharmacopeial designation, excipient role, or manufacturing use is provided. Do not use for diagnostic or therapeutic purposes.

General note (literature/practice)

• While monoclonal antibodies can serve as therapeutics or analytical reagents in regulated environments, this specific product lacks any clinical/diagnostic claims or compliance statements in the provided data.

Physical Properties

Item‑specific properties

• Appearance, concentration, formulation buffer, protein concentration, isotype, clonality details: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General values for mouse IgG antibodies (literature/typical)

• Typical molecular mass: ~150 kDa for intact IgG; Fab ~50 kDa; Fc ~50 kDa (literature).
• Isoelectric point (pI): commonly 6–9 depending on isotype and sequence (literature range).
• Solubility: Highly soluble in aqueous buffers (e.g., PBS, TBS) with stabilizers (e.g., BSA, trehalose) (general practice).
• Stability modifiers: Glycerol (30–50% v/v) can permit storage at −20 °C without freezing; sugars (trehalose/sucrose) can protect during lyophilization (general).
• Extinction coefficient (A280): Sequence‑dependent; not provided for this item.

Important distinctions

• Do not treat protein antibodies with small‑molecule physical constants (bp/mp, logP) — not applicable.
• For exact buffer composition, concentration, and isotype of Ab125320, consult the product label or CoA; not specified herein.

Quality and Grades

Item‑specific quality details

• Grade/purity, isotype, clonality number/clone ID, host species confirmation, and QC application data: Not specified for this item; refer to CoA/Spec Sheet.

General guidance on antibody quality (literature/practice)

• Monoclonal antibodies (mAbs) provide epitope‑specific recognition with lot‑to‑lot consistency relative to polyclonals. Typical QC includes SDS‑PAGE purity, concentration by A280, endotoxin (for certain uses), and application validation (WB, IHC, IF, IP, ELISA).
• Carrier proteins/preservatives: Formulations may contain BSA, gelatin, trehalose, glycerol, and/or sodium azide. These can affect downstream assays (e.g., BSA interferes with certain coupling chemistries). Composition is not specified for Ab125320.
• Species reactivity and cross‑reactivity profiling are central quality attributes for target‑specific antibodies; these data are not provided here for Ab125320.
• Documentation: For clone lineage, immunogen description, and recommended dilutions, consult the Certificate of Analysis and Specification Sheet when supplied.

Implications for end‑users

• If performing conjugations (biotin, HRP, fluorophores), verify that the antibody is carrier‑free and azide‑free. Not specified for this item; confirm before use.
• For quantitative applications, obtain lot‑specific extinction coefficient or concentration from CoA to enable accurate normalization.

Reaction and Applications

Not typically applicable

• This product is a mouse monoclonal antibody intended for immunoassays and protein detection, not for use as a small‑molecule reagent in synthetic or catalytic chemical reactions.

Relevant laboratory applications (general/literature)

• Common use cases for RNASEH2A antibodies include Western blotting (WB), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and ELISA to detect endogenous RNASEH2A in cell/tissue lysates.
• Typical workflows involve antigen retrieval for IHC (if formalin‑fixed), blocking to reduce nonspecific binding, primary incubation with the anti‑RNASEH2A mAb, and detection with an anti‑mouse secondary antibody.

Item‑specific limitations

• Validated applications, species reactivity, and recommended dilutions for Ab125320 are not specified for this item; refer to CoA/Spec Sheet before experimental use.

Reaction Conditions

Not applicable

• Classical reaction condition descriptors (solvent, temperature, catalysts, yields) do not apply to antibody reagents.

General assay conditions (literature/practice)

• WB: Primary incubation often 1–16 h at 4–25 °C; blocking in 3–5% BSA or milk; washing with PBST/TBST.
• IF/IHC: Primary incubation 1–16 h; antigen retrieval and permeabilization as required; counterstain nuclei with DAPI.
• IP: Incubate lysate with antibody 1–4 h at 4 °C; capture with Protein A/G resin depending on isotype (isotype not specified for this item). Optimize empirically.

Item‑specific data (dilutions, incubation times, species): Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

Item‑specific safety information

• GHS classification, signal word, pictograms, and H‑statements: Not specified for this item; refer to the SDS.
• Research use: For research use only (as provided). Not for diagnostic, therapeutic, or clinical use.

General laboratory guidance (literature/practice)

• PPE: Wear lab coat, safety glasses, and appropriate disposable gloves when handling antibody solutions and any accompanying preservatives.
• Preservatives: Some antibody formulations contain sodium azide or Proclin; avoid contact with acids to prevent hydrazoic acid formation and flush drains with ample water if disposal is permitted locally. Composition for this item is not specified — check SDS/label.
• Biological origin: Although recombinant or hybridoma‑derived, treat as potentially biohazardous; avoid ingestion, inhalation, or skin exposure. Use good microbiological practices.
• First aid (overview): In case of skin/eye contact, rinse with water. If inhaled or swallowed, move to fresh air and seek medical attention if symptoms persist. Consult SDS for detailed instructions.
• Incompatibilities: Avoid repeated freeze–thaw cycles; do not mix with oxidizing agents or strong acids/bases unless validated for your workflow.
• Spill response: Absorb with inert material, clean with detergent, and dispose according to institutional biosafety procedures.

Always defer to the SDS and institutional EHS policies for authoritative safety information.

Solvent Selection

Applicability to this product

• As a protein reagent, this monoclonal antibody is formulated and used in aqueous buffers; conventional organic solvent selection is not typically relevant.

General buffer considerations (literature/practice)

• Common storage/working buffers: PBS (pH ~7.2–7.4) or TBS (pH ~7.4–8.0) with optional 0.02–0.05% sodium azide and stabilizers (e.g., 10–50% glycerol for −20 °C storage; sugars for lyophilized cakes). The exact formulation for Ab125320 is not specified.
• Additives: 0.1%–5% BSA or 1% skim milk can reduce nonspecific binding in immunoassays; include Tween‑20 (0.05%–0.1%) for washing buffers in WB/ELISA/IF as appropriate.
• Avoid: High concentrations of organic solvents (e.g., >10–20% methanol, DMSO, acetonitrile) can denature antibodies unless specifically validated for assay protocols.

Selection tips

• Choose PBS for most cell‑free immunoassays; TBS can be preferable when phospho‑specific interactions are sensitive to phosphate. For metal‑dependent assays, phosphate may chelate; consider HEPES or Tris.

Item‑specific note

• Buffer composition and any stabilizers for Ab125320 are not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item‑specific storage (from Product Data)

• Storage conditions: Store at −20 °C; avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (cold‑chain shipment).

General guidance (literature/practice)

• Aliquoting: Upon receipt, aliquot into single‑use volumes to prevent freeze–thaw damage. Use low‑protein‑binding tubes.
• If liquid formulation: Consider adding sterile glycerol to a final 30–50% v/v for −20 °C storage without freezing, if compatible with the supplied formulation (composition for this item not specified; check label/SDS).
• If lyophilized: Reconstitute with sterile water or buffer (e.g., PBS) to the recommended concentration; gently invert to mix; avoid vigorous vortexing. Reconstitution volume and target concentration are not specified for this item.
• Short‑term handling: Keep on ice during use. Do not leave at room temperature for extended periods.
• Long‑term storage: For multi‑year storage, −80 °C is often preferred if formulation permits; validate stability for your workflow.
• Freeze–thaw: Avoid; if unavoidable, limit to a single thaw per aliquot.

Unspecified parameters

• Formulation buffer, presence of carrier proteins/preservatives, and recommended reconstitution solvent/volume: Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

Item‑specific facts (from Product Data)

• SKU: Ab125320
• Product name: RNASEH2A Mouse mAb
• Type/host: Mouse monoclonal antibody (mAb) against RNASEH2A (target indicated by product name)
• CAS, CID, InChIKey, SMILES, molecular formula, molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General description (literature/knowledge)

• RNASEH2A is the catalytic A subunit of the heterotrimeric Ribonuclease H2 complex (A/B/C). The complex cleaves RNA in RNA:DNA hybrids and initiates ribonucleotide excision repair in genomic DNA.
• Mouse monoclonal antibodies are typically immunoglobulin G (IgG) class with two identical heavy chains and two light chains, organized into variable (V) regions for antigen binding and constant (C) regions for effector function.
• Structural features (antibody): Two antigen‑binding fragments (Fabs) each containing VH/VL domains forming a paratope that recognizes an epitope on RNASEH2A; one Fc region mediates isotype‑specific interactions.

Notes on “structure depiction”

• Antibodies are large glycoproteins; conventional 2D small‑molecule structural descriptors (SMILES, InChI) are not applicable. Sequence and epitope information are not provided for this item.

Synthetic Utility

Not applicable

• As a biological macromolecule used for target protein detection, this mouse monoclonal antibody is not used as a building block or reagent in organic synthesis.

Related laboratory utility (general)

• Utility is in immunoassays (WB, IF, IHC, IP, ELISA) for detecting RNASEH2A, mapping protein–protein interactions (co‑IP), and monitoring biochemical fractionation. Application validation details for Ab125320 are not specified.

Target Specificity

Item‑specific details (from Product Data)

• Target: RNASEH2A (Ribonuclease H2 subunit A)
• Antibody type: Mouse monoclonal antibody (mAb)

Not specified for this item; refer to CoA/Spec Sheet

• Clone ID and isotype: Not specified
• Epitope/immunogen: Not specified
• Species reactivity (human/mouse/rat/etc.): Not specified
• Cross‑reactivity: Not specified
• Applications validated (WB, IHC, IF, IP, ELISA): Not specified
• Positive control recommendations: Not specified

General context (literature)

• RNASEH2A is a nuclear protein and catalytic subunit of the RNase H2 complex involved in RNA:DNA hybrid processing.

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.