Rogosa SL Broth - BioReagent

Cat. No.: R1528783

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Panoramica

For testing of Lactobacillus in oral cavity, vagina, and feces

Specifications

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent

Product Line

Prodotti biologici

Application Protocols

No item-specific, validated application protocols are provided in the Product Data. For general use, consult internal SOPs and the CoA/Spec Sheet for this SKU.

Literature/general workflow outline (for context only; not product specifications):

• Medium preparation:
  • Weigh the supplier-recommended mass of dehydrated powder per liter of purified water. Dissolve with stirring; adjust pH only if directed. Autoclave under validated conditions. Allow to cool to 45–50 °C before aseptic additions.
• Inoculation and incubation:
  • Aseptically inoculate with the Lactobacillus culture (e.g., 1–2% v/v from an actively growing pre-culture). Incubate at the strain-appropriate temperature (often 30–37 °C) under microaerophilic/anaerobic conditions as needed.
• Monitoring:
  • Track growth by OD600, viable counts, or acidification (pH monitoring). Subculture to selective agar (e.g., Rogosa SL Agar) for isolation.
• Storage of prepared medium:
  • Short-term hold at 2–8 °C is common in practice to limit contamination and degradation; avoid repeated heat cycles. Discard if turbidity or contamination appears in uninoculated controls.

Always adapt conditions to your strains and comply with institutional biosafety requirements. For this specific item, defer to the lot-specific documentation for exact preparation instructions.

Biological Roles

Rogosa SL Broth itself has no biological role inside organisms; rather, it provides an ex vivo environment tailored to the physiology of lactobacilli.

Literature/general context:

• Lactobacillus physiology: Lactobacilli are fermentative, acid-tolerant Gram-positive bacteria that thrive in environments with abundant carbohydrates and low redox potential. They require divalent cations (Mg2+, Mn2+), B-vitamins, amino acids/peptides, and often benefit from fatty acid esters as growth factors.
• Selective pressure: The broth’s acidic pH and acetate/citrate system suppress many non-target bacteria while accommodating aciduric lactobacilli. Tween 80 (polysorbate 80), when present (literature), can supply oleic acid derivatives, enhancing growth for some species.
• Metabolic outputs: During cultivation, lactobacilli convert carbohydrates to lactic acid (homo- or heterofermentative pathways), further decreasing pH and reinforcing selectivity.
• Applications in microbiology: The medium supports isolation, maintenance, and physiological studies (e.g., carbohydrate utilization, stress tolerance) of lactobacilli. However, nutrient complexity may mask subtle auxotrophies; defined media are preferable for metabolic quantitation.

Item-specific biological claims: None provided. This product is for research use only and is not intended for clinical diagnosis or therapy.

Buffer Applications

Rogosa SL Broth is a complete, complex growth medium, not a dedicated buffering reagent. While it contains buffering components (e.g., phosphate, acetate/citrate systems, literature) and is formulated at an acidic pH for selectivity, it is not typically used to prepare analytical buffers or electrophoresis running buffers.

• If you require a defined buffer system, select an appropriate Good’s buffer (e.g., MES for pH ~6.0, citrate for pH ~3–6) or phosphate buffers, prepared independently at the desired ionic strength and pH.
• For microbial cultivation where pH control is crucial, consider pairing the medium with controlled aeration and pH-stat bioreactor operation rather than relying solely on intrinsic buffering capacity.

Green Alternatives

As a microbiological medium, Rogosa SL Broth is water-prepared and does not directly implicate common green-chemistry solvent tradeoffs. Nevertheless, sustainability considerations apply to its use and disposal.

Greener practice suggestions (literature/general):

• Water and energy efficiency:
  • Prepare only the volume needed to reduce waste; consider small-batch autoclaving or validated media sterilization cycles to minimize energy use.
  • Where validated, use lower-temperature/shorter-time sterilization for heat-sensitive supplements via sterile filtration and aseptic addition, reducing repeated autoclave cycles.
• Waste minimization:
  • Segregate biologically contaminated waste and autoclave before disposal; avoid adding chemical disinfectants that complicate wastewater treatment unless required by local SOPs.
  • Use reusable glassware when possible; if using single-use plastics, choose recyclable options and consolidate sterilization loads.
• Sourcing and composition:
  • If available, select products with responsible sourcing of complex nitrogen components (e.g., certified suppliers), and request documentation on supply chain transparency.
• Comparison to alternatives:
  • For broader lactic acid bacteria cultivation, MRS Broth may reduce selective agents but could increase total biomass, potentially affecting downstream waste volume. For higher selectivity at low pH, Rogosa SL can reduce the need for additional antibiotics as selective agents (tradeoff: acidic effluent management).

Always follow institutional environmental health and safety policies for culture media preparation and disposal.

Pharmaceutical Uses

No therapeutic or clinical uses are claimed. For research and manufacturing support only.

Literature/general roles in pharmaceutical and bioprocess settings:

• Microbiological quality control (QC): Selective enrichment of Lactobacillus spp. from raw materials, probiotic products, and in-process samples to verify identity and viability.
• Strain banking and propagation: Pre-culture of lactobacillus strains used in development of probiotic candidates or as production hosts for biochemical pathways (research stage), prior to transfer into defined or production media.
• Environmental monitoring/contamination assessments: Detection of lactic acid bacteria in fermentation facilities where their presence may be beneficial or undesirable depending on the process.
• Method validation: As part of growth promotion testing, Rogosa SL formulations may serve as selective media in compendial or in-house methods (where appropriate). Always verify against relevant pharmacopeial requirements; compositions can differ among suppliers.

Item-specific pharmacopeial status and compliance: Not specified for this item; refer to CoA/Spec Sheet and your quality unit for method suitability.

Physical Properties

• Item-specific appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Item-specific numeric specifications (BP, MP, density, solubility, refractive index, pH of prepared medium): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general characteristics for Rogosa SL Broth (for context only; not product specifications):

• Physical form: Dehydrated, free-flowing powder blend; typically hygroscopic due to salts and carbohydrates.
• Solubility: Intended to be fully water-soluble at the recommended preparation strength (literature), forming a clear to slightly opalescent solution before sterilization; may become slightly turbid after autoclaving/cooling due to complex components.
• Prepared medium pH: Commonly adjusted to an acidic range (~5.4 at 25 °C) for selectivity toward lactobacilli (value varies by supplier and lot; literature).
• Color (prepared): Light amber to tan (literature, varies with components and autoclave cycle).
• Hygroscopicity: Medium powders readily absorb moisture; keep tightly sealed (general practice).

Notes for professional use:

• Dissolution is typically improved by gradual addition to warm purified water under vigorous stirring to minimize clumping and foaming.
• Filter sterilization is generally avoided for complex media; autoclaving is standard unless heat-sensitive supplements are added aseptically post-sterilization (literature practice).

Quality and Grades

• Item-specific grade: BioReagent (as provided). No additional grade qualifiers or stabilizers are specified for this catalog item.
• What BioReagent typically implies (general):
  • Manufactured and handled to meet biological research needs, focusing on consistency, low bioburden, and performance in standard laboratory procedures.
  • May include supplier-defined limits for contaminants relevant to microbiological growth (e.g., inhibitory residues), and verification via growth promotion testing—consult CoA for this lot-specific information.
• Purity/assay details: Not specified for this item; refer to CoA/Spec Sheet.
• UV, metals, peroxide, water content specifications: Not specified for this item; refer to CoA/Spec Sheet.
• Performance considerations for Rogosa SL Broth (literature/general):
  • Selectivity arises from the acidic pH and presence of acetate/citrate salts and manganese/magnesium ions, supporting lactobacilli while suppressing many non-targets.
  • Lot-to-lot consistency is important for comparative studies; retain CoAs and record autoclave parameters and supplements used.
• Practical guidance:
  • Validate each new lot with a small-scale growth test using a representative Lactobacillus strain relevant to your workflow.
  • For downstream analytical applications (e.g., metabolomics), pre-test background signals since complex media can contribute matrix interferences (general advice).

Reaction and Applications

This item is a selective microbiological growth medium; it is not used as a reagent in chemical synthesis. The relevant “applications” are microbiological.

Literature/general applications of Rogosa SL Broth:

• Selective enrichment and enumeration of Lactobacillus spp. from complex samples (foods, probiotics, fermentation matrices), leveraging its acidic pH and selective salts to suppress competing flora.
• Pre-culture and propagation of lactobacilli prior to downstream assays (e.g., carbohydrate fermentation profiling, genome sequencing, metabolite analysis), recognizing that complex media can influence metabolite backgrounds.
• Quality control in production of lactobacillus-containing products, where the broth serves as a selective pre-enrichment before plating onto Rogosa SL Agar or other selective agars (literature practice).

Practical notes (general):

• The broth’s low pH and acetate/citrate content are key to selectivity; verify target strain tolerance before large-scale culture.
• For microaerophilic or anaerobic lactobacilli, incubate under reduced oxygen (e.g., anaerobic jars, gas packs) and consider adding reducing agents as appropriate—added aseptically after sterilization if heat-labile.
• When transitioning from broth to agar, maintain consistent formulation to avoid selection drift between steps.
• Record autoclave cycle and cooling time; overprocessing can darken the medium and mildly impact growth performance (general observation).

Reaction Conditions

In lieu of chemical reaction conditions, the relevant operational parameters are microbiological preparation and incubation conditions.

Item-specific preparation parameters (grams per liter, target pH, sterilization instructions): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance for Rogosa SL Broth:

• Preparation:
  • Suspend the recommended quantity of dehydrated medium in purified water with vigorous stirring. Literature formulations for Rogosa SL Broth are commonly in the approximately 50–60 g/L range, but values vary by supplier and lot; follow the label/CoA for this item.
  • Heat gently to aid dissolution; avoid overheating that can induce caramelization of carbohydrates.
  • Adjust to the specified acidic pH (around mid-5 range at 25 °C, literature) only if directed by the supplier; many formulations are self-adjusting.
  • Sterilize by autoclaving (typical cycles: 121 °C for 15–20 min; verify vessel volume and institutional SOP). Cool to 45–50 °C before adding heat-labile supplements aseptically.
• Incubation (for lactobacilli):
  • Temperature: Commonly 30–37 °C depending on species/strain (literature).
  • Atmosphere: Microaerophilic to anaerobic conditions often beneficial; use anaerobic jars or controlled incubators as needed.
  • Duration: 18–72 h to reach late exponential/stationary phase, varying with strain and inoculum size.
• Quality checks: Include uninoculated controls for sterility and, where applicable, growth promotion controls with reference strains.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the product SDS for authoritative information.
• General hazards (literature/general for dehydrated culture media):
  • Low inherent toxicity expected, but dust may cause mechanical irritation to eyes/respiratory tract.
  • Contains complex organic nutrients; treat as laboratory chemical mixture. Avoid inhalation and contact.
• PPE recommendations (good lab practice):
  • Safety glasses, lab coat, and nitrile gloves when handling dry powder or prepared broth.
  • Use in a clean environment to avoid microbial contamination; employ BSC if handling live cultures in BSL-appropriate settings.
• Handling tips:
  • Minimize dust generation; use dedicated scoops/spatulas. Reseal promptly to limit moisture uptake.
  • Prepare solutions with purified water; avoid cross-contamination by using sterile vessels and aseptic technique where required.
• Incompatibilities/conditions to avoid (general):
  • Moisture and high humidity (caking, reduced shelf life); strong oxidizers (can degrade organic constituents).
• First aid (overview; defer to SDS):
  • Eye/skin contact: Rinse with water. If irritation persists, seek medical attention.
  • Inhalation: Move to fresh air; seek attention if symptoms persist.
  • Ingestion: Rinse mouth; seek advice if unwell. Not intended for human or animal consumption.
• Research Use Note: For research use only.

Solvent Selection

This product is a water-prepared microbiological medium rather than a solute selected for organic reaction solvents.

• Item-specific solvent details: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance for Rogosa SL Broth:

• Primary solvent: Water (Type I/II ultrapure or equivalent) for dissolution and medium preparation.
• Miscibility: Components are formulated to be water-soluble at the recommended concentration; not intended for organic solvents.
• Practical tips:
  • Use freshly produced, low-endotoxin water when sterility and sensitive downstream assays matter.
  • Avoid hard/ion-rich water, which can shift medium pH and ionic strength, affecting selectivity and growth.
• When to choose Rogosa SL vs alternatives (literature):
  • Choose Rogosa SL for selective enrichment of Lactobacillus spp. at low pH.
  • Consider MRS Broth for broader lactic acid bacteria cultivation at pH ~6.2 (less selective), or Tomato Juice Broth for certain lactobacilli with different nutritional profiles.
• Sterilization solvent context:
  • Autoclaving in water is standard. If heat-labile supplements are needed, dissolve them in sterile water and add aseptically after cooling the autoclaved broth to ~45–50 °C (literature practice).

Storage and Reconstitution

• Storage conditions (item-specific): Room temperature (as provided). Protect from moisture; keep container tightly closed. Shipped under normal conditions.
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet and product label for expiry/retest date.
• Reconstitution/preparation (item-specific grams per liter, target pH): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance:

• Keep the powder in a dry, cool place, away from direct sunlight. Use desiccant in secondary containment if ambient humidity is high.
• After opening, promptly reseal and, if feasible, store in a low-humidity cabinet. Record the date opened and maintain good inventory rotation (FIFO).
• For preparation, dissolve the supplier-recommended mass in purified water with vigorous stirring. Sterilize by autoclaving; allow to cool before adding heat-labile supplements aseptically.
• Prepared broth is best used fresh. If storage is necessary, hold at 2–8 °C for a short period (e.g., days, as allowed by your SOP) and inspect for contamination or precipitation before use.
• Avoid freeze–thaw of prepared medium; repeated temperature cycling can cause component precipitation and performance variability.

Research Use Note: For research use only.

Structure and Identity

• Item type: Complex dehydrated microbiological medium (Rogosa SL Broth); not a single defined chemical entity.
• Item-specific identifiers:
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey / SMILES: Not applicable (mixture); Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula / Molecular weight: Not applicable for a mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Composition note (literature/general): Rogosa SL media are formulated to selectively cultivate lactobacilli by employing a low initial pH, acetate/citrate salts, manganese and magnesium ions, phosphate buffering, and a nonionic surfactant (e.g., polysorbate 80), together with complex nitrogen sources (peptones/yeast extract) and a fermentable carbohydrate (often glucose). Exact composition varies by supplier and format (broth vs agar).
• Structural features: As a complex blend, Rogosa SL Broth contains a mixture of organic and inorganic constituents (amino acids/peptides, carbohydrates, organic acids/salts, phosphates, and trace metals). No single 2D structure applies.
• Identity confirmation (practice guidance): For complex media identity is typically confirmed by:
  • Lot-linked CoA showing composition/specification conformance.
  • QC growth promotion testing with representative Lactobacillus strains (literature practice), not provided here for this specific item.
• Naming: “Rogosa SL” refers to the selective low-pH formulation described by M. Rogosa et al. for enrichment of lactobacilli (literature).

Synthetic Utility

Not applicable. Rogosa SL Broth is not used as a reagent or building block in chemical synthesis.

For users planning coupled chemical analyses (literature/general guidance):

• Be aware that complex media components can interfere with analytical methods (e.g., LC–MS background, derivatization reactions in GC analysis). Consider media blanks and sample cleanup (protein precipitation, SPE) before analysis of culture supernatants.
• If the goal is to study specific metabolic conversions, you may switch to chemically defined media to reduce background and improve mass balance.
• For isotopic labeling experiments, defined media with labeled substrates (e.g., 13C-glucose) are preferred over complex broths to maintain tractable carbon/nitrogen sources.

Target Specificity

Not applicable. This product is a microbiological growth medium, not a biological targeting reagent (e.g., antibody, ligand, or probe). No antigen/epitope/clone/isotype information is relevant or provided.