GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human S100A10 Antibody, Monoclonal (6F4E6D5C10), could be used for WB, IF/ICC and so on.
Specifications
Product Name
S100A10 Mouse mAb - Primary antibody, specific to S100A10, Rabbit IgG
Synonyms
Calpactin I light chain | Calpactin-1 light chain | Cellular ligand of annexin II | p10 protein | p11 | S100 calcium-binding protein A10 | 42C antibody | AA409961 antibody | AL024248 antibody | Annexin II ligand antibody | Annexin II ligand, calpactin I,
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Host species
Mouse
Specificity
S100A10
Immunogen
Recombinant protein of mouse S100A10.
Positive Control
WB: HeLa, A431, TH-29, A549, HUVEC and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
S100A10 Mouse mAb (Ab126219) - Western Blot All lanes: S100A10 Mouse mAb (Ab126219) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 13 kDa Exposure time: 58.4 s
S100A10 Mouse mAb (Ab126219) - IF/ICC Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100A10 Mouse mAb (Ab126219) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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