Schaedler Agar - BioReagent

Cat. No.: S1528622

QUALITÀ & PUREZZA BioReagent

Storage

Room temperature

Shipped In

Normal

Panoramica

For isolation and cultivation of anaerobic bacteria; vitamin K1 and defibrinated sheep blood may be added as needed

Specifiche

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent

Product Line

Prodotti biologici

Application Protocols

General workflow for preparing and using Schaedler Agar (literature guidance; not item-specific):

1. Preparation

• Weigh 40–50 g of dehydrated medium per 1 L of purified water. Suspend with stirring.
• Heat to boiling to dissolve completely; avoid scorching. Adjust pH if required per formulation guidance.
• Autoclave at 121 °C for 15 minutes. Allow to cool to 45–50 °C.
• Aseptically add sterile supplements (e.g., hemin ~5–10 mg/L, vitamin K1 ~1 mg/L, L-cysteine 0.05–0.1%, and/or 5% defibrinated sheep blood) as needed by your protocol.

2. Plate pouring and pre-reduction

• Pour 20–25 mL per 100 mm plate; let solidify.
• Place plates in an anaerobic chamber or anaerobic jar with gas pack for 12–24 h to pre-reduce.

3. Inoculation and incubation

• Inoculate using aseptic technique. Minimize oxygen exposure for strict anaerobes by working in an anaerobic workstation or transferring rapidly to anaerobic conditions.
• Incubate at 35–37 °C for 24–72 h; extend as needed for slow growers.

4. Quality control (recommended)

• Perform growth promotion tests with representative anaerobes (e.g., Bacteroides fragilis) and inhibition checks using appropriate organisms.

5. Disposal

• Autoclave all used plates and cultures before disposal per biosafety and institutional guidelines.

Biological Roles

Schaedler Agar functions as a nutrient and environmental platform to support microbial life, especially obligate anaerobes. It is not a single biomolecule but a formulated micro-ecosystem (general literature):

• Nutritional support:
  • Peptones/yeast extract supply amino acids, peptides, vitamins, and trace factors to support biosynthesis and energy metabolism.
  • Carbohydrates (e.g., glucose) provide fermentable substrates, supporting ATP generation via anaerobic pathways.
• Anaerobic growth facilitation:
  • Hemin (a porphyrin) supports cytochrome-associated enzymes in certain anaerobes.
  • Vitamin K1 participates in menaquinone biosynthesis/electron transport in Bacteroides and related genera.
  • Reducing agents (e.g., L-cysteine) and pre-reduction of plates lower redox potential (Eh), protecting oxygen-sensitive enzymes.
• Structural role of agar:
  • Provides a non-nutritive, inert gel matrix enabling colony isolation and morphologic differentiation.
• Community studies:
  • Used to recover members of complex microbiotas (gut/oral/fecal/environmental) for downstream molecular characterization, metabolite profiling, and strain banking (research use only).

Because composition is complex and performance is organism-dependent, empirical verification (growth promotion testing with representative strains) is recommended for each lot and use case.

Buffer Applications

Schaedler Agar is a complete culture medium rather than a discrete buffer system. Dedicated buffer preparation is not typically applicable.

• Intrinsic buffering (general literature): Many formulations include phosphate or similar salts providing modest buffering around neutral pH (~6.8–7.2 after sterilization), which stabilizes pH during anaerobic growth.
• Practical notes:
  • If additional buffering is required for specific fermentations or metabolomics studies, adjust the aqueous phase before sterilization using sterile, compatible buffers (e.g., phosphate) while monitoring potential growth effects.

For standard buffer recipes (HEPES, PBS, MOPS), prepare separately; do not assume equivalence to Schaedler medium.

Green Alternatives

Although green chemistry frameworks apply primarily to synthetic reagents and solvents, media selection can consider sustainability and safety.

• Resource considerations (general):
  • Dehydrated media minimize cold-chain energy use (shipped/stored at room temperature per Product Data) and reduce packaging mass versus ready-to-pour plates.
  • Prepare only the quantity needed to limit waste from expired plates.
• Animal-origin components:
  • Traditional Schaedler formulations may use animal-derived peptones and blood. If reducing animal-origin inputs is a priority, consider animal-free peptone sources or blood-free alternatives where microbiological objectives permit (performance tradeoffs likely).
• Reducing agents and supplements:
  • Use minimal effective levels of cysteine or other reductants to lower chemical load, while maintaining anaerobic performance.
• Waste and sterilization:
  • Autoclave waste to inactivate biological hazards; segregate plastics for recycling where permitted after decontamination.

Comparison snapshot (general, non-item-specific):

• Schaedler Agar: Broad anaerobe recovery; may require animal-derived supplements; robust but component-intensive.
• Brucella Agar with hemin/VK1: Comparable anaerobe recovery; similar component profile; often used with blood.
• Vegetable peptone-based anaerobe agars: Lower animal-origin footprint; may show altered growth for certain fastidious taxa.

Selection should balance performance with sustainability goals for the specific research question.

Pharmaceutical Uses

This product is designated For research use only in the Product Data and is not claimed to meet pharmacopeial specifications.

General information for context (not item-specific; no clinical claims):

• In pharmaceutical and biotechnology quality control, nonselective anaerobic media are used for environmental monitoring, bioburden assessments, and growth promotion testing of sterility media. Where compendial compliance is required, pharmacopeial media or validated equivalents must be used.
• Schaedler-type media can serve in development laboratories and method feasibility studies investigating recovery of anaerobic organisms from materials or processes.
• Formulation role: solid surface for colony isolation, purity checks of anaerobic production organisms, and preparation of biomass for analytical characterization.

Users requiring GMP/compliance should verify suitability and perform method validation/verification against applicable standards (e.g., USP/EP/JP) and internal SOPs.

Physical Properties

Item-specific physical specifications are not provided in the Product Data. Where useful, typical values are given from literature for Schaedler Agar or similar anaerobe media and should not be taken as product specifications.

• Appearance (dehydrated): Not specified for this item; refer to CoA/Spec Sheet. (Literature: free-flowing beige to tan powder.)
• Solubility: Designed to dissolve/disperse in purified water during medium preparation; insoluble in organic solvents (general knowledge).
• Prepared medium characteristics (literature, typical):
  • Final concentration: commonly 40–50 g/L of dehydrated medium in water (prior to agar setting)
  • pH at 25 °C: ~7.0 ± 0.2 after sterilization (exact pH is formulation-dependent)
  • Gelation temperature: agar solidifies upon cooling to ~35–45 °C and melts at ~85–90 °C (literature, for agar)
  • Color: light to medium amber; may darken with blood supplementation (literature)
• Density/refractive index/logP/pKa: Not applicable to complex media; not defined for mixtures.
• Thermal behavior (general): Dehydrated powder is stable at room temperature when kept dry; prepared plates are heat-sensitive and should not be re-autoclaved.

For precise acceptance criteria (appearance, pH range, fill weight), consult the product CoA/Spec Sheet.

Quality and Grades

• Item-specific grade (from Product Data): BioReagent.

What BioReagent typically implies (general guidance):

• Intended for research and laboratory use; not for human or animal use, drug, diagnostic, or household purposes.
• Manufactured to support reproducible experimental outcomes in life-science workflows. Typical controls may include:
  • Consistency of nutrient profile and gelling characteristics between lots
  • Microbiological performance testing against defined organisms (e.g., recovery of representative anaerobes) — details are manufacturer-specific
  • Moisture content limits and pH of prepared medium within defined ranges

Clarifications specific to this listing:

• Purity percentages, stabilizers, and inhibitory contaminant limits are Not specified for this item; refer to CoA/Spec Sheet.
• No medical/clinical or pharmacopeial compliance is claimed in the Product Data.

Best practices for quality-sensitive applications:

• Use high-purity water (e.g., ASTM Type 1 or 2) for reconstitution; avoid chlorine/chloramine.
• Pre-reduce plates/broth for strict anaerobes to ensure expected performance.
• Record lot numbers and perform in-house QC (growth promotion testing) when media performance is critical to study outcomes.

Reaction and Applications

Not a chemical reagent for synthetic transformations. Instead, Schaedler Agar is used in microbiological applications. Below are research uses and practical considerations (general literature):

• Primary purpose: Nonselective, enriched solid medium to isolate and cultivate obligate anaerobes and other fastidious organisms from environmental, gut, oral, or clinical research samples.
• Typical enhancements:
  • Supplementation with hemin (X factor), vitamin K1, L-cysteine (as a reducing agent), and 5% defibrinated sheep blood.
  • Antibiotic supplements can be added for selective isolation (e.g., kanamycin–vancomycin for Bacteroides), recognizing these alter spectrum.
• Practical tips for success:
  • Pre-reduction: Place poured plates in an anaerobic chamber or anaerobic jars with gas packs for 12–24 h before inoculation of strict anaerobes.
  • Inoculation and incubation: Perform in an anaerobic workstation or quickly transfer inoculated plates into anaerobic jars; incubate at 35–37 °C for 24–72 h (species-dependent).
  • Reducing environment: Include reducing agents in the medium or use catalyst sachets to achieve <1% O2.
• Downstream applications:
  • Colony morphology assessment, purity streaking, and biomass preparation for genomics, metabolomics, or antimicrobial susceptibility testing (research use only).

For detailed preparation steps, see Application Protocols.

Reaction Conditions

Chemical reaction conditions are not applicable. For microbiological culture conditions (general literature; not item-specific):

• Reconstitution: Commonly 40–50 g of dehydrated medium per liter of purified water. Heat with agitation to dissolve and melt agar.
• Sterilization: Autoclave at 121 °C for 15 minutes. Do not over-sterilize to avoid Maillard browning and nutrient degradation.
• Supplements: After cooling to 45–50 °C, aseptically add sterile-filtered hemin, vitamin K1, L-cysteine, and/or 5% defibrinated sheep blood as required.
• Plate pouring: Pour into sterile petri dishes; allow to solidify. Target plate depth ~4 mm (≈20–25 mL for 100 mm plates) for consistent diffusion and colony morphology.
• Pre-reduction: Place plates in an anaerobic chamber or jars with anaerobic gas packs for 12–24 h before inoculating strict anaerobes.
• Incubation: 35–37 °C under anaerobic conditions (e.g., 85% N2 / 10% H2 / 5% CO2, or commercial gas packs). Incubation time 24–72 h or longer depending on organism.

These are general guidelines; adjust per organism and laboratory SOPs. Always verify with growth promotion tests.

Safety and Handling

GHS hazard classification and statements are not specified in the Product Data. Always consult the product SDS for authoritative safety information.

• GHS classification/Pictograms: Not specified for this item; refer to SDS.
• Likely hazards (general for dehydrated culture media):
  • Low acute toxicity; dust may cause mechanical irritation to eyes/respiratory tract.
  • Nutrient medium will support microbial growth; contamination/used plates constitute a biohazard.
• PPE and hygiene:
  • Wear lab coat, gloves, and safety glasses.
  • Avoid dust generation; handle powders in a fume hood or with local exhaust if dusty.
  • Practice aseptic technique to prevent unintended cultivation of pathogens.
• Incompatibilities (general):
  • Strong oxidizers and moisture. Keep container tightly closed and dry.
• First aid (general):
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses; obtain medical advice if irritation persists.
  • Skin contact: Wash with soap and water.
  • Ingestion: Rinse mouth; seek medical attention as needed.
• Sterilization/biological safety:
  • Autoclave prepared media and all used plates/broths before disposal.
  • When culturing anaerobes or clinical isolates, use appropriate biosafety level practices.

Storage per Product Data: Room temperature; protect from humidity. Ship under normal conditions.

Solvent Selection

This product is a dehydrated culture medium intended to be reconstituted with purified water; solvent selection among organic media is not applicable.

Practical guidance (general literature):

• Recommended solvent: High-purity water (Type 1 or 2). Avoid deionizers that leach organic amines or residual sanitizers; these can inhibit fastidious anaerobes.
• Water quality considerations:
  • Resistivity ≥ 1–10 MΩ·cm, TOC low, and free of heavy metals and residual oxidants.
  • Degassing or deoxygenating water (e.g., boil and cool under N2) can improve recovery of strict anaerobes.
• Miscibility: The dehydrated medium disperses in water; agar hydrates upon heating to boil before sterilization.

When to choose Schaedler Agar vs alternatives (general):

• Choose Schaedler for broad, nonselective recovery and enumeration of obligate anaerobes (e.g., Bacteroides, Clostridium spp.), especially when supplemented with hemin and vitamin K1 and/or blood.
• Consider Brucella Agar with blood or CDC Anaerobe 5% Sheep Blood Agar for clinical workflows that require specific performance standards.
• For aerotolerant or mixed flora, Columbia or Tryptic Soy agars (with supplements) may suffice.

Storage and Reconstitution

• Storage conditions (from Product Data): Room temperature. Ship under normal conditions.
• Container care: Keep tightly closed in a cool, dry place. Protect from humidity to prevent caking and premature degradation.
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution (general literature; not item-specific):

• Dissolve the dehydrated powder in purified water at the manufacturer-recommended concentration (commonly 40–50 g/L).
• Heat with stirring to fully dissolve; do not overboil.
• Sterilize by autoclaving at 121 °C for 15 min. Cool to 45–50 °C before adding heat-labile supplements (e.g., hemin, vitamin K1, blood).
• Pour plates aseptically; allow to solidify. For strict anaerobes, pre-reduce plates 12–24 h in an anaerobic environment before use.

Prepared media handling (general):

• Store poured plates at 2–8 °C in sealed sleeves or bags to limit dehydration and oxidation; use as soon as practical (typically within 2–4 weeks, per internal QC timelines).
• Avoid repeated warming/cooling cycles. Discard plates showing contamination, desiccation, or pH/appearance drift.

Research Use Only: As stated in Product Data; not for use in diagnostic procedures.

Structure and Identity

Schaedler Agar is a complex, undefined culture medium formulated to support the growth of obligate anaerobes and other fastidious microorganisms. As a dehydrated blend of multiple organic and inorganic components, it does not have a single molecular structure.

• Item identity (from Product Data):
  • SKU: S1528622
  • Product Name: Schaedler Agar
  • Grade/Purity: BioReagent
  • Storage Conditions: Room temperature; shipped under normal conditions
  • Research Use Note: For research use only
• Composition (general literature; not item-specific):
  • Typically contains casein/soy peptones and yeast extract (amino acids, peptides, growth factors)
  • Carbohydrate source (e.g., dextrose)
  • Enrichment factors for anaerobes (e.g., hemin and vitamin K1)
  • Mineral salts/buffering components
  • Agar as the solidifying agent
• Structure descriptors:
  • Molecular formula: Not applicable (complex mixture)
  • Molecular weight: Not applicable (complex mixture)
  • SMILES / InChIKey: Not applicable (mixture)
• Structural features (descriptive):
  • Heterogeneous blend of peptides, carbohydrates, porphyrin (hemin), lipid-soluble vitamin (K1), and polysaccharide agar. No stereodefined single entity; composition optimized to create a reduced, nutrient-rich microenvironment for anaerobic culture.

Note: Exact formulation and concentrations are manufacturer-specific; consult the product CoA/Spec Sheet for definitive composition.

Synthetic Utility

Not applicable to chemical synthesis. Schaedler Agar is a microbiological growth medium and is not used as a reagent, catalyst, or building block in organic or inorganic synthesis.

For research involving metabolic biotransformations, the medium can support growth of anaerobic biocatalysts that perform transformations ex vivo; however, the medium itself is not a synthetic reagent.

Target Specificity

Target specificity data (e.g., antigen/epitope, clone, isotype, species reactivity) are not applicable. This product is a culture medium, not an antibody or affinity reagent.

Item-specific target data: Not specified for this item; refer to CoA/Spec Sheet if any organism performance claims are provided by the manufacturer.

Domande frequenti

How should this product be stored?

Store at room temperature.