SMAD5 Mouse mAb - Anticorpo primario, specific to SMAD5, Mouse IgG1

Cat. No.: Ab128237

GRADE & PURITY ExactAb™, Validated, 1.6 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG1

Clone Number

4B10-B10-B6

Conjugation

Unconjugated

Panoramica

Mouse anti Human SMAD5 Antibody, Monoclonal (4B10-B10-B6), could be used for WB, IF/ICC, Flow and so on.
Application:
WB: 1/500-1/1000
IF/ICC: 1/50-1/200    
Flow: 1/50-1/100
Protein Function:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Specifications

Product Name

SMAD5 Mouse mAb - Anticorpo primario, specific to SMAD5, Mouse IgG1

Sinonimi

Dwfc | hSmad5 | JV5-1 | JV5-1DKFZp781O1323 | MAD homolog 5 | MAD, mothers against decapentaplegic homolog 5 (Drosophila) | MADH5 | MADH5mothers against decapentaplegic homolog 5 | mothers against decapentaplegic homolog 5 | mothers against decapentaplegic

Specifiche e purezza

ExactAb™, Validated, 1.6 mg/mL

Specie ospite

Mouse

Specificità

SMAD5

Immunogeno

Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. (AA 1-465).

Controllo positivo

WB: HeLa, SK-N-SH, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. Flow: Jurkat cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

1.6 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant SMAD5 Antibody (Ab128237)

Recombinant SMAD5 Antibody (Ab128237) - Western Blot
All lanes: Recombinant SMAD5 Antibody (Ab128237) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1.0 s

IF/ICC: Recombinant SMAD5 Antibody (Ab128237)

Recombinant SMAD5 Antibody (Ab128237) - IF/ICC
Immunocytochemistry analysis of Smad5 in HeLa cells using Recombinant SMAD5 Antibody (Ab128237).

Flow Cytometry: Recombinant SMAD5 Antibody (Ab128237)

Recombinant SMAD5 Antibody (Ab128237) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Recombinant SMAD5 Antibody (Ab128237) (red). Black line histogram represents the isotype control, normal mouse IgG.

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

IF/ICC

1/50 - 1/200

Flow Cytometry

1/50-1/100

Obiettivi associati (umani)

SMAD5 Tbio Mothers against decapentaplegic homolog 5 (0 Activities)

Discover Target: SMAD5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SMAD5?Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.