Sodium Sulfite Agar - BioReagent

Cat. No.: S1528359

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Panoramica

For cultivation of Yersinia pestis.

Specifications

Specifiche e purezza

BioReagent

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent

Product Line

Prodotti biologici

Application Protocols

• Item-specific tested applications: Not specified for this item; refer to CoA/Spec Sheet.

• General workflow for agar media (literature/good practice)

  • Preparation
    • Weigh the manufacturer-recommended g/L of dehydrated medium into deionized water.
    • Heat with stirring until fully dissolved; adjust volume; check preliminary pH if required by SOP.
    • Autoclave using a validated cycle; cool to ~50 °C.
  • Plate pouring
    • Aseptically dispense into sterile Petri dishes; allow to solidify. Dry plates if necessary in a biosafety cabinet to remove surface moisture.
  • Inoculation and incubation
    • Streak or spread inoculum as per method; incubate under conditions suitable for the target organisms (temperature, atmosphere). For microaerophilic/anaerobic work, ensure appropriate redox/anaerobic systems as required.
  • Quality controls
    • Include positive/negative control strains and record colony morphology/expected differential reactions according to the validated method.

• Disposal

  • Autoclave used plates and media as biohazardous waste prior to disposal, following institutional guidelines.

Biological Roles

• Applicability

  • This product is a culture medium, not a biological macromolecule. It does not have intrinsic cellular roles but supports growth/selection of microorganisms in vitro.

• General/literature context

  • Agar serves as a non-nutritive gelling agent; it is minimally metabolized by most microbes, providing a stable surface.
  • Sodium sulfite (SO3^2−) is a reducing agent that can lower redox potential and interact with oxygen and reactive aldehydes; in certain formulations, sulfite can participate in differential reactions associated with sulfite-reducing or sulfur-metabolizing microbes. The biological implications (e.g., colony blackening with sulfide precipitation when iron salts are present) depend on the medium’s full composition.
  • Peptones/yeast extracts (if present) supply amino acids, peptides, vitamins, and growth factors that underpin biomass formation and metabolic activity.

• Practical note

  • The exact biological performance characteristics (selectivity, differential readouts, growth range) are formulation-specific and should be verified via growth promotion testing against relevant control strains. For this item, composition details are Not specified; refer to CoA/Spec Sheet.

Buffer Applications

• Not typically applicable
  • Sodium Sulfite Agar is a microbiological growth medium, not a buffering reagent. While the reconstituted medium will have a defined pH, it is not intended as a general-purpose buffer system.
  • For pH-sensitive work, use dedicated buffers (e.g., phosphate, HEPES) prepared separately according to standard recipes.

Green Alternatives

• Context

  • As a ready-to-reconstitute dehydrated medium, this product already reduces solvent waste relative to made-from-scratch formulations and minimizes cold-chain requirements (shipped at ambient).

• Greener practice suggestions (general)

  • Scale preparation to immediate need to reduce biohazard waste and energy use from autoclaving surplus plates.
  • Use energy-efficient autoclave cycles validated for sterility assurance and avoid over-sterilization.
  • Consider reusable glassware for media preparation where compatible with your biosafety program to reduce single-use plastics.
  • Implement proper segregation and autoclave deactivation to prevent unnecessary incineration volume.

• Comparison (general perspective)

  • Dehydrated media vs. pre-poured plates:
    • Dehydrated media: lower packaging mass, reduced shipping footprint, flexible batch sizing.
    • Pre-poured plates: convenience at the cost of higher packaging waste and potential refrigerated shipping.

• Chemicals of concern

  • If working toward “greener” microbiology, avoid unnecessary selective agents (e.g., heavy metals) unless required by the method; however, only adjust formulation if validated for your application.

Pharmaceutical Uses

• Not a pharmaceutical excipient

  • This product is intended for research use only and is not formulated to compendial pharmacopeia standards for drug manufacturing.

• Relevant lab/industrial contexts (general)

  • Comparable microbiological media are used in environmental monitoring, bioprocess development, and QC microbiology for detecting or enumerating specific microbial groups, subject to validated methods.
  • If media are required for GMP workflows, ensure the product meets appropriate quality documentation, traceability, and growth promotion requirements. For this item, compendial/GMP status is Not specified; refer to CoA/Spec Sheet.

Physical Properties

• Item-specific facts (from Product Data)

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Other physicochemical specifications (mp, bp, density, pH of 1% solution, g/L preparation, etc.): Not specified for this item; refer to CoA/Spec Sheet.

• General properties of dehydrated agar media (literature, for context only)

  • Typical form: free-flowing, tan-to-beige powder that hydrates in water and gels upon cooling after melt/sterilization.
  • Agar gel point typically around ~35–40 °C and melt point ~85–90 °C (literature, varies with salts/solutes).
  • Solubility: Dispersible in water; requires heating to dissolve agar fully; insoluble in organic solvents.
  • Redox character: presence of sulfite (SO3^2−) provides a reducing environment that may interact with dissolved oxygen and reactive carbonyls; exact redox capacity depends on formulation.

• Practical notes

  • Prepare using deionized water, heat to dissolve, then sterilize (typically by autoclaving) and cool to pour; exact grams per liter and pH target must follow the product’s label/CoA.
  • Final gel strength and clarity depend on agar content and any supplementary components (indicators, selective agents).

Quality and Grades

• Item-specific facts (from Product Data)

  • Grade: BioReagent

• What BioReagent typically implies (general)

  • The BioReagent designation is commonly used for materials suitable for biological and biochemical research, with controls on impurities that could inhibit microbial growth, enzymatic activity, or cell viability. For complex media, this generally means consistent nutrient quality, low inhibitory contaminants, and batch-to-batch reproducibility.

• Composition control in complex media (general)

  • Dehydrated agar media comprise agar, nutrients/peptones, salts, and in this case a sulfite component. Critical quality parameters include: nutrient lot consistency, ash/total solids, gel strength, clarity, and pH after reconstitution.

• Documentation and verification

  • Exact formulation, pH targets, and preparation grams per liter are product-specific and may vary across manufacturers. For this item: Not specified for this item; refer to CoA/Spec Sheet.
  • For regulated workflows, retain CoA and perform incoming QC checks (reconstituted pH, gel strength, sterility, growth promotion tests) aligned to your lab’s SOPs.

• Stabilizers/indicators

  • If indicators or selective agents are included in a given Sodium Sulfite Agar formulation, their identity and concentration should be verified against the product label/CoA prior to use.

Reaction and Applications

• Item-specific applications (from Product Data)

  • Manufacturer applications: Not specified beyond general category (life sciences). For research use only.

• General/literature applications for “Sodium Sulfite Agar” (context; verify against your protocol)

  • Used as an agar-based culture medium in microbiology in which sodium sulfite functions as a reducing/indicator-participating component. Such media are often used to support or differentiate sulfite-reactive or sulfite-reducing microorganisms. Specific target groups and performance depend on the exact formulation (which may or may not include additional selective agents and indicators).
  • Examples in the literature include media where sulfite participates in precipitation reactions (e.g., with iron salts to form black precipitates of metal sulfides under microbial activity) or acts as a redox modulator to lower oxidation–reduction potential.

• Practical tips (general)

  • Follow the product’s labeled grams per liter and pH target; deviations will alter growth characteristics and selectivity.
  • Achieve complete dissolution by heating with agitation; autoclave per SOP. Pour at ~50 °C to minimize condensation.
  • For differential outcomes, ensure any required supplements/indicators are added at the correct temperature to avoid decomposition.
  • Perform growth promotion testing (GPT) with relevant positive/negative control strains to confirm lot performance for your application.

Reaction Conditions

• Microbiological preparation conditions (general guidance; not product-specific)

  • Reconstitution: Add the labeled grams per liter of dehydrated medium to deionized water. Heat with agitation until fully dissolved.
  • Sterilization: Autoclave using a validated cycle (e.g., 121 °C, 15 min; literature guidance). Allow for cooling to ~50 °C before pouring plates to prevent excessive condensation and to protect heat-labile supplements.
  • Additives: If required by your method, aseptically add sterile-filtered supplements/indicators after cooling below ~55 °C.
  • Solidification: Leave plates undisturbed to set; store inverted to limit surface moisture.

• Performance checks

  • Verify final pH at 25 °C (literature practice) and run growth promotion tests with appropriate positive and negative control organisms based on your method.

• Item-specific numeric conditions are Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

• Item-specific hazard data (from Product Data)

  • Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.

• General laboratory safety for dehydrated media (literature/good practice)

  • PPE: lab coat, nitrile gloves, safety glasses; avoid inhalation of dust. Use a dust mask/respirator if significant powder handling is expected and local risk assessment requires it.
  • Handling: Minimize dust generation; handle in a well-ventilated area. When heating to dissolve agar, beware of superheating and bumping; swirl carefully and use appropriate flasks with headspace.
  • Thermal hazards: Hot agar solutions can cause burns; pour plates at ~45–55 °C to limit condensation and thermal risk (general guidance).
  • Chemical considerations: Formulations containing sulfite may release trace SO2 under strong acid; avoid strong acids and oxidizers. Do not mix with incompatible chemicals unless validated.
  • Microbiological hygiene: After preparation and inoculation, treat materials as biohazardous according to institutional biosafety procedures. Decontaminate media and disposables by autoclaving before disposal.

• First-aid overview (general)

  • Inhalation of dust: move to fresh air; seek medical attention if irritation persists.
  • Skin/eye contact: rinse with water for at least 15 minutes; remove contaminated clothing; seek medical advice if irritation continues.
  • Ingestion: rinse mouth; obtain medical attention.

• Always consult the product-specific SDS for authoritative hazard information and emergency measures.

Solvent Selection

• Applicability

  • This product is a dehydrated microbiological medium intended to be reconstituted in water. Organic solvent selection is not applicable.

• Practical guidance

  • Use high-purity deionized/distilled water for reconstitution to avoid inhibitory ions or chlorine.
  • Avoid buffers or salts not specified by the manufacturer, as they may alter osmolarity, redox balance (sulfite), and gelation.
  • If supplements (e.g., antibiotics, vitamins) are required, prepare them as sterile aqueous stock solutions and aseptically add after autoclaving and cooling to ~50 °C, unless the supplement is heat-stable per its datasheet.

Storage and Reconstitution

• Item-specific facts (from Product Data)

  • Storage conditions (dehydrated medium): Room temperature.
  • Shipping: Normal (ambient).

• General storage guidance (literature/good practice)

  • Keep container tightly closed, in a cool, dry place away from moisture and strong oxidizers/acids. Protect from ambient humidity to prevent clumping and premature degradation of reducing components.
  • Avoid prolonged exposure to light and heat. Use clean, dry scoops to prevent contamination.

• Reconstitution guidance (not product-specific; follow label/CoA for exact values)

  • Dissolve the manufacturer-specified grams per liter in deionized water with heating and stirring until clear.
  • Sterilize by autoclaving. If heat-labile supplements are required, sterile-filter them and add after cooling the base medium to ~50–55 °C.
  • Pour plates or dispense into sterile containers as appropriate. Allow to solidify and store plates inverted at 2–8 °C if long-term storage is needed, unless your SOP specifies otherwise.

• Shelf life

  • Dehydrated media are typically stable for many months when kept dry; the precise expiry/retest date and any lot-specific precautions are Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

• Item-specific facts (from Product Data)

  • Product name: Sodium Sulfite Agar (SKU: S1528359)
  • Type: Dehydrated microbiological medium (complex mixture; not a single chemical entity)
  • Grade/Purity: BioReagent
  • Storage: Room temperature; shipped under Normal conditions
  • CAS, CID, InChIKey, SMILES: Not applicable to complex media; not assigned
  • Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• General description (literature)

  • Sodium Sulfite Agar denotes an agar-based culture medium containing sodium sulfite and other components (peptones, salts, agar) designed to support microbial growth; exact composition is product-specific.
  • Structural features: as a mixture, it comprises a polysaccharide gelling matrix (agar) plus low-molecular-weight nutrients and salts, including a sulfur(IV) reductant (sulfite) that can influence redox conditions and enable differential reactions with sulfite-reactive indicators (when present in a given formulation).

Synthetic Utility

• Not applicable
  • As a complex culture medium, Sodium Sulfite Agar is not used as a synthetic building block or reagent in organic synthesis. For chemical synthesis needs, consider purchasing individual reagent-grade chemicals (e.g., sodium sulfite, agar, peptones) with defined specifications.

Target Specificity

• Not applicable
  • This product is not an antibody, probe, or assay kit with molecular target specificity. Any selectivity relates to the microbiological formulation (nutrients, inhibitors, redox conditions) and must be confirmed by validated methods. For this item, specific selectivity claims are Not specified; refer to CoA/Spec Sheet.