SPR Mouse mAb - Anticorpo primario, specific to SPR

Cat. No.: Ab129035

GRADE & PURITY Validated, KO Validation, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

consiglio, Umano

Isotype

Mouse IgG1

Clone Number

OTI4F5

Conjugation

Unconjugated

Panoramica

Mouse anti Human SPR Antibody, Monoclonal (OTI4F5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

SPR Mouse mAb - Anticorpo primario, specific to SPR

Sinonimi

OTTHUMP00000160199 antibody | SDR38C1 antibody | Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody | Sepiapterin reductase antibody | Short chain dehydrogenase/reductase family 38C, member 1 antibody | SPR antibody | SPRE_HUMAN an

Specifiche e purezza

KO Validation, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.

Specie ospite

Mouse

Specificità

SPR

Immunogeno

Recombinant full length protein of human SPR.

Controllo positivo

WB: HepG2, A549 and HeLa cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, liver, lung carcinoma, ovary adenocarcinoma, pancreas, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY

Coniugazione

Unconjugated

Grado

ExactAb™, KO Validation, Validated

SDS-PAGE

150 kDa

Metodo di purificazione

Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - Western Blot
All lanes: SPR Mouse mAb (Ab129035) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 14.6 s

Flow: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - Flow
Flow cytometric Analysis of Jurkat cells, using SPR Mouse mAb (Ab129035) (Red), compared to a nonspecific negative control antibody, (Blue).

Flow: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - Flow
HEK293T cells transfected with either pCMV6-ENTRY SPR overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by SPR Mouse mAb (Ab129035), and then analyzed by flow cytometry.

IF/ICC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) – IF/ICC
SPR Mouse mAb (Ab129035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human colon tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: SPR Mouse mAb (Ab129035)

SPR Mouse mAb (Ab129035) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using SPR Mouse mAb (Ab129035). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

Obiettivi associati (umani)

SPR Tchem Sepiapterin reductase (0 Activities)

Discover Target: SPR

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SPR Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.