SQSTM1 Mouse mAb - Anticorpo primario, specific to SQSTM1, Mouse IgG1

Cat. No.: Ab129090

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Mouse, Umano

Isotype

Mouse IgG1

Clone Number

C1

Conjugation

Unconjugated

Panoramica

Mouse anti Human SQSTM1 Antibody, Monoclonal (C1), could be used for WB, IF/ICC, IHC, Flow Cytometry and so on.

Specifications

Product Name

SQSTM1 Mouse mAb - Anticorpo primario, specific to SQSTM1, Mouse IgG1

Sinonimi

A170 | Autophagy Receptor P62 | DMRV | EBI3-associated protein of 60 kDa | EBI3-associated protein p60 | EBIAP | FTDALS3 | NADGP | ORCA | OSIL | oxidative stress induced like | p60PDB3 | p62 | p62B | Paget disease of bone 3 | PDB3 | phosphotyrosine indepe

Specifiche e purezza

Carrier Free, ExactAb™, Validated, High Performance, See COA

Meccanismi biochimici e fisiologici

Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitina

Specie ospite

Mouse

Specificità

SQSTM1

Immunogeno

Recombinant SQSTM1 expressed in E.coli (AA 136-400).

Controllo positivo

WB: HeLa and A549 cell lysates. IF/ICC: HeLa cells. IHC: Human breast cancer tissue. Flow Cytometry: A549 cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A + Protein G purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - Western Blot
All lanes: SQSTM1 Mouse mAb (Ab129090) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 54 kDa
Exposure time: 1.5 s

IF: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - IF
Immunocytochemistry analysis of SQSTM1/p62 (green) in U87-MG cells using SQSTM1 Mouse mAb (Ab129090), and cells were counterstained with DAPI (blue).

IHC: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SQSTM1 Mouse mAb (Ab129090). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Flow Cytometry: SQSTM1 Mouse mAb (Ab129090)

SQSTM1 Mouse mAb (Ab129090) - Flow Cytometry
Human A549 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-TritonX-100, then stained with 10 µg/mL SQSTM1 Mouse mAb (Ab129090, red histogram) or Isotype control antibody (green histogram), followed by 1µg/mL FITC conjugated Anti-mouse IgG Secondary Antibody.

Applicazione

• Applicazione: Dilution info
• WB:

  0.01-2 µg/mL ;

• IHC:

  2-20 µg/mL;

• Flow Cytometry:

  10µg/mL

Obiettivi associati (umani)

SQSTM1 Tbio Sequestosome-1 (0 Activities)

Discover Target: SQSTM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SQSTM1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.