SRPK2 Mouse mAb - Anticorpo primario, specific to SRPK2, Mouse IgG2a

Cat. No.: Ab129203

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2a

Clone Number

K29040_13A1

Conjugation

Unconjugated

Panoramica

Mouse anti Human SRPK2 Antibody, Monoclonal (K29040_13A1), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/200
Protein Function
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

Specifications

Product Name

SRPK2 Mouse mAb - Anticorpo primario, specific to SRPK2, Mouse IgG2a

Sinonimi

Serine kinase SRPK2 antibody | Serine/arginine rich protein specific kinase 2 antibody | Serine/arginine-rich protein-specific kinase 2 antibody | Serine/threonine protein kinase SRPK2antibody | SFRS protein kinase 2 antibody | SFRSK2antibody | SR protein

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

SRPK2

Immunogeno

Recombinant protein of human SRPK2 (AA 321-460)

Controllo positivo

WB: HeLa and MCF7 cell lysates. IHC: Human cerebellum tissue.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: SRPK2 Mouse mAb (Ab129203)

SRPK2 Mouse mAb (Ab129203) - Western Blot
All lanes: SRPK2 Mouse mAb (Ab129203) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 99 kDa
Exposure time: 100.0 s

IHC: SRPK2 Mouse mAb (Ab129203)

SRPK2 Mouse mAb (Ab129203) - IHC
IHC analysis of cerebellum tissue by SRPK2 Mouse mAb (Ab129203).IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SRPK2 Mouse mAb (Ab129203) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Purkinje cells are positively stained at the cytoplasm of cell body and dentrite.

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/200 ;

Obiettivi associati (umani)

SRPK2 Tchem SRSF protein kinase 2 (0 Activities)

Discover Target: SRPK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SRPK2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.