Synthetic Blocking Buffer, ELISA - Ready-to-use (RTU)

Cat. No.: S494401

GRADE & PURITY Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Panoramica

Product Characteristics 

The Synthetic Blocking Buffer, ELISA is a ready-to-use solution for blocking of the remaining free binding sites after microplate coating in ELISAs. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal, improving the signal-to-noise ratio. 

The Synthetic Blocking Buffer, ELISA reduces the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA.

Composition & Properties 

The Synthetic Blocking Buffer is a ready-to-use buffer. The buffer contains no proteins, and crossreactivity is therefore avoided.

Working Procedure 

1.Coat the microtiter plate according to the usual procedure. It is recommended to dilute the biomolecule in a fresh carbonate buffer, pH 9.6. 

2.After plate coating, rinse the microwells with 3 x 300 µl PBS buffer. 

3.Dispense the Synthetic Blocking Buffer into each well making sure wells are completely full. 

4.Incubate for 5-10 minutes at room temperature. 

5.Rinse with 3 x 300 µl washing buffer optimized for the assay. 

6.Continue the plate processing using the usual procedure.

Tips & Tricks 

• In case of a demand for stabilization of coated ELISA plates, our one step blocker & stabilizer, WellChampion (cat. no. W494467) is recommended. 

• For membranes, the Synthetic Blocking Buffer, Blotting (cat. no. S494457) is recommended. 

Handling & Storage 

• Store solution at 2-8 °C.

Specifications

Specifiche e purezza

Ready-to-use (RTU)

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light

Spedito in

Wet ice

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Proprietà chimiche e fisiche

• Sensibilità: Light sensitive

Domande frequenti

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.