TelN Protelomerase, CAS No.2747966-35-2

CAS: 2747966-35-2 Cat. No.: T767021

GRADE & PURITY Bioactive, Recombinant, Suitable for molecular biology, EnzymoPure™, ≥10 U/μl

Accession #

Q37967

Expression system

E. coli

Bioactivity

≥10 U/μl

Panoramica

T767021	Component	250 U	1000 U	Storage
T767021A	TelN Protelomerase	25 μL	100 μL	-20°C. Avoid freeze/thaw cycle
T767021B	10×TelN Reaction Buffer	250 μL	1 mL	-20°C. Avoid freeze/thaw cycle

TelN Protelomerase, whose Chinese name is TelN Prokaryotic telomerase, is cloned from phage N15, which cuts dsDNA at the recognition site TelRL (56bp), The TelRL site consists of a palindromic sequence TelO in the middle and a palindromic sequence R3 and L3 at both ends of 14bp (Fig.1), and leaves a covalently closed end at the cleavage site, thus effectively converting circular DNA into linear DNA with hairpin ends (Fig.2).

Source
E.coli
Enzyme Activity Definition
The amount of enzyme required to completely cut 0.5 μg linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) for 30 min in a 50 μl system at 30°C was defined as 1 unit of activity (U).
Protocol
(1) Configure the mixture as shown in the following table:

Component	volume
dsDNA	X μl
10×TelN Reaction Buffer	2 μl
TelN Protelomerase	1 μl
Nuclease-free Water	To 20 μl

(2) Shake the mixed system gently up and down, and centrifuge briefly.
(3) Incubate at 30℃ for 30 min.
(4) Incubation at 75℃ for 5 min terminated the reaction.
Cautions
(1) This product is for scientific research purposes only and shall not be used for other purposes (since 2022.05.31).
(2) In some application scenarios, there are patent restrictions on the use of this enzyme.

Specifications

Product Name

TelN Protelomerase, CAS No.2747966-35-2

Sinonimi

TelN Protelomerase

Grado

Bioactive, Recombinant, Suitable for molecular biology, EnzymoPure™

Specifiche e purezza

Bioactive, Recombinant, Suitable for molecular biology, EnzymoPure™, ≥10 U/μl

Bioattività

≥10 U/μl

Numero d'ordine

Q37967

CAS

2747966-35-2

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥10 U/μl

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle

Definizione dell'unità

One unit (U) is defined as the amount of enzyme required to completely digest 0.5 μg of BsaI-linearized pMiniT-TelN control plasmid (containing 313 fmol TelN recognition site(s)) in a 50 μL reaction mixture after incubation at 30 °C for 30 min.