Tissue/Cell RNA Extraction Kit (One-Step Extraction Method)

Cat. No.: T1527583

GRADE & PURITY BioReagent

Panoramica

RNA can be sourced from a variety of materials, and numerous methods exist for its extraction and preparation. Commonly used techniques include the phenol method, the detergent method, and the guanidine hydrochloride method, among which the phenol method is the most widely employed. This product (RNA extraction reagent) contains phenol and guanidinium isothiocyanate, among other components, which rapidly lyse cells or tissues and inactivate nucleases, thereby preserving RNA integrity. After the addition of chloroform and centrifugation, the solution separates into three phases: the upper aqueous phase (colorless), the interphase, and the lower organic phase (red). Total RNA is recovered from the upper aqueous phase by isopropanol precipitation, DNA is precipitated from the interphase with ethanol, and proteins are precipitated from the lower organic phase with isopropanol. This product is suitable for rapid isolation of total RNA from various tissues or cells, and can handle either small samples (50–100 mg tissue or 5×10⁶ cells) or large samples (>1 g tissue or >10⁷ cells). The extracted total RNA is of high quality and can be used for Northern blot, Dot blot, poly(A)⁺ RNA selection, in vitro translation, RNase protection assays, and molecular cloning experiments. The kit features broad applicability, simple operation (the entire process can be completed within 1 hour), high purity, and low contamination. This reagent is intended for research use only and is not suitable for clinical diagnosis or other applications.

Specifications

Sinonimi

Yeast RNA Extraction Kit

Specifiche e purezza

BioReagent

Grado

BioReagent

Sensibilità

Light Sensitive

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Room temperature

Spedito in

Normal,Wet ice

Stabilità e conservazione

During shipment, each component is individually packaged and transported under temperaturecontrolled conditions corresponding to its storage requirement (Room Temperature/ 2-8°C ) to maintain optimal product performance. For a single order containing comp

Contents & Storage

Storage Unit No.	Storage Unit Name	Component No.	Component Name	Appearance	50T	Storage
T1527583NT	Tissue/Cell RNA Extraction Kit (One-Step Extraction Method)(RT Components)	T1527583A	RNA Separation Solution	Liquid	10ml	Room temperature.Store in the dark.
		T1527583B	RNA Precipitation Solution	Liquid	50ml	Room temperature
		T1527583C	RNA Wash Solution	Liquid	50ml	Room temperature
		T1527583D	RNase-free ddH₂O	Liquid	10ml	Room temperature
		T1527583E	RNA Storage Solution	Liquid	10ml	Room temperature.Store in the dark.
T1527583WT	Tissue/Cell RNA Extraction Kit (One-Step Extraction Method)(2-8℃ Components)	T1527583F	RNA Extraction Reagent	Liquid	50ml	2-8℃.Store in the dark.

Note: Each component is individually packaged according to its storage temperature (Room temperature/2‑8 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 12 months from the date of manufacture.

Domande frequenti

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.