TPC2-A1-N - 10mM in DMSO , CAS No.136186-07-7

CAS: 136186-07-7 Cat. No.: T655607 Formula: C₁₇H₉Cl₂F₃N₂O₂ Peso molecolare: 401.17

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

TPC2-A1-N is a powerful and Ca 2+ -permeable agonist of two pore channel 2 (TPC2) , which plays its role by mimicking the physiological actions of NAADP. TPC2-A1-P reproducibly evokes significant Ca 2+ responses from TPC2 ( EC 50 =7.8 μM ), and the effect can be blocked by several TPC blockers. TPC2-A1-N can be used to probe different functions of TPC2 channels in intact cells

In Vitro

Two-pore channels (TPC1-3) are ancient members of the voltage-gated ion channel superfamily. TPCs are expressed throughout the endo-lysosomal system and regulates the trafficking of various cargoes. TPC2 can mediate different physiological and possibly pathophysiological effects depending on how it is activated. The ion selectivity of TPC2 is not fixed but rather agonist-dependent. TPC2 is a unique example of an ion channel that conducts different ions in response to different activating ligands. TPC2-A1-N (10 μM) reproducibly evokes Ca 2+ signals, and TPC2-A1-N response reachs its plateau faster than TPC2-A1-P. The EC 50 in full concentration-effect relationships for the plateau response is 7.8 μM for TPC2-A1-N in a cell line stably expressing TPC2 L11A/L12A . TPC2-A1-N (10 μM) evokes Ca 2+ influx through the TPC2 pore evokes Ca 2+ signals in cells expressing TPC2L11A/L12A but not TPC2 L11A/L12A/L265P . Additionally, the responses to TPC2-A1-N can be selectively blocked by the identified TPC2 blockers Tetrandrine , Raloxifene , and Fluphenazine by removal of extracellular Ca 2+. In endo-lysosomal patch-clamp experiments, TPC2-A1-N (30 μM) elicits currents using Na + as the major permeantion, in vacuolin-enlarged endo-lysosomes isolated from isolated from HEK293 cells transiently expressing human TPC2 (hTPC2) but not in cells expressing TPC1. In endo-lysosomal patch-clamp experiments, TPC2-A1-N (30 μM) induces larger currents in endo-lysosomes isolated from cells expressing a gain-of-function variant of TPC2 (TPC2 M484L ) compared to the wild-type isoform, and exhibits an EC 50 value of 0.6 μM for TPC2-A1-N. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:EC50: 10.5 μM (Ca 2+ current response from TPC2)

Specifications

Specifiche e purezza

10mM in DMSO

Meccanismi biochimici e fisiologici

TPC2-A1-N is a powerful and Ca 2+ -permeable agonist of two porexa0channelxa02xa0(TPC2) , which plays its role by mimicking the physiological actionsxa0ofxa0NAADP. TPC2-A1-P reproduciblyxa0evokes significant Ca 2+ responses from TPC2 ( EC 50 =7.8 μM ), an

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

• Peso molecolare: 401.17

Domande frequenti

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 136186-07-7, the molecular formula is C17H9Cl2F3N2O2, and the molecular weight is 401.17 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.