Transhydrogenase-2 Activity Assay Kit (UV Micro Method)

Cat. No.: T1522134
Disponibile su ordine
GRADE & PURITY BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
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Size
Germania (EU)
USA*
Price
Qty
100T
T1522134-100T
Su ordinazione · 8–12 settimane
286,27€
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Why this grade

BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Panoramica

TH is located on the inner mitochondrial membrane and is also known as mitochondrial complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH, thereby regulating the balance of mitochondrial NAD(H) and NADP(H). The reverse reaction is referred to as TH‑2, which catalyzes the conversion of NADPH and NAD⁺ to NADP⁺ and NADH. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide (APAD⁺) as a substitute for NAD⁺, TH‑2 catalyzes the reduction of APAD⁺ to APADH, which has a characteristic absorption peak at 375 nm. The TH‑2 activity is calculated by measuring the rate of increase in absorbance at 375 nm.

Specifications

Specifiche e purezza
BioReagent
Grado
BioReagent
Applicazione
Enzyme activity assay
Detection instrument
Microplate reader,UV Spectrophotometer
Detection wavelength
375nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C
Spedito in
Wet ice
Stabilità e conservazione
Store at 2-8℃ for 6 months.

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

Domande frequenti

What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

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