Triton™ X-100 - for electrophoresis , CAS No.9002-93-1
Disponibile
SKU
T109028
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection
Application
Triton™ X-100 has been used:
• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling
Maggiori Informazioni
| CAS | 9002-93-1 |
|---|---|
| Sinonimi | Igepal CA-210 | CAS_118-96-7 | PDSP1_001087 | Ethanol,1,3,3-tetramethylbutyl)phenoxy]- | 2-(4-(2,4,4-Trimethylpentan-2-yl)phenoxy)ethan-1-ol | Octoxynol-1 | Triton X 305 | P-TERT-OCTYLPHENYL (2-HYDROXYETHYL)ETHER | AT25387 | Igepal CA 630 | FT-0673247 | T |
| Specifiche e purezza | for electrophoresis |
| Informazioni legali | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Condizioni di conservazione di stoccaggio | Room temperature |
| Spedito in | Normal |
| Product Description | General description Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection Application Triton™ X-100 has been used: • In immunohistochemistry for staining the Flat-mount retinas • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay • To permeabilise cells during Immunofluorescent microscopic studies • As a positive control in LDH assay to determine the cell membrane integrity • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure • For the preparation of outer membrane protein exctract • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells • In the treatment of tissue sections for Immunofluorescence labeling |
| Pubchem Sid | 508813680 |
| Numero Mdl | MFCD00128254 |
| Numero CE | 618-344-0 |
| PubChem CID | 5590 |
| Formula molecolare | C8H17C6H4(OCH2CH2)nOH |
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