Trypsin/Lys-C Mix (MS), CAS No.9002-07-7

CAS: 9002-07-7 Cat. No.: T1523244 Numero EC: 607-831-3

QUALITÀ & PUREZZA Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™, ≥99%(HPLC), Trypsin ≥15000 U/mg protein, Lys-C ≥25 U/mg protein

Expression system

E. coli

Panoramica

This product contains trypsin and Lys-C protease, which are recombinantly expressed metalloproteases. Trypsin specifically cleaves peptide bonds at the C-terminus of arginine (R) and lysine (K) residues with ultra-fast kinetics; Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine (K) residues rapidly. Since trypsin cannot digest arginine and lysine at the same rate, many lysine residues are missed. Additionally, post-translational modification (PTM) on lysine or adjacent strongly hydrophobic amino acids (e.g., proline, P) at the C-terminus can also cause missed cleavages. The trypsin/Lys-C protease cocktail is suitable for processing complex protein samples that are difficult to digest. It is applied in protein characterization, single-cell omics, and large-cohort proteomics research. Reconstitution Solution: Reconstitute with 40 μL of 50 mmol/L acetic acid to obtain a concentration of 0.5 μg/μL. pH Range: The protease cocktail exhibits optimal activity at pH 7–9. Digestion Protocol: Recommended Digestion Buffer: 20 mmol/L Tris, 50 mmol/L ABC, or HEPES buffer (pH 7–9). Denatured Protein Sample: Use a protease-to-protein ratio of 1:50 (w/w). Adjust the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 37°C for 30 minutes.

Specifiche

Product Name

Trypsin/Lys-C Mix (MS), CAS No.9002-07-7

Grado

Adatto alla spettrometria di massa (MS), Recombinant, EnzymoPure™

Specifiche e purezza

EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),Trypsin ≥15000 U/mg protein, Lys-C ≥25 U/mg protein

CAS

9002-07-7

Numero della Commissione Enzimi

EC 3.4.21.4;EC 3.4.21.50

Tipo di molecola

Enzima

Product Line

Prodotti biologici

Stoccaggio e spedizione

Concentrazione

Trypsin ≥15000 U/mg protein, Lys-C ≥25 U/mg protein

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Definizione dell'unità

Trypsin: The amount of trypsin that hydrolyzes 1.0 μmol of BAEE per minute at 253 nm, under conditions of pH 7.8 and 25°C. Lys-C: The amount of rLys C that hydrolyzes 1.0 μmol of T-G-P rLys Nitroanilid per minute at 405 nm, under conditions of pH 7.8 and

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥99% purity, determined by HPLC. Lot-specific values are stated on the Certificate of Analysis.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What does suitable for mass spectrometry (MS) mean?

suitable for mass spectrometry (MS) indicates that the chemical has been purified and tested specifically for use in liquid chromatography–mass spectrometry. Key specifications typically include controlled UV absorbance at common detection wavelengths, low non-volatile residue, controlled moisture content, and verified lot-to-lot consistency. suitable for mass spectrometry (MS) is used for mobile phases, sample preparation and analytical standards in this workflow.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9002-07-7.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.