UK-101 , CAS No.1000313-40-5

CAS: 1000313-40-5 Cat. No.: U650416 Formula: C₂₅H₄₈N₂O₅Si Peso molecolare: 484.74

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

UK-101 is a potent and selective immunoproteasome β1i (LMP2) inhibitor with an IC 50 value of 104 nM, displays 144- and 10-fold selectivity over β1c ( IC 50 =15 μM) and β5 subunit ( IC 50 =1 μM), respectivey UK-101 induces cell apoptosis and can be used for the study of prostate cancer .

In Vitro

UK-101 (2-8 μM; 24 hours) induces cell cycle arrest and increases the number of the PC-3 cells arrest in G1 phase of the cell cycle. UK-101 (2-8 μM; 24 hours) induces cell apoptosis, shows a minimal increase in late apoptosis, but has no significant increase in early apoptosis. UK-101 (1-8 μM; 24 hours) induces cells accumulation in the G1 phase of the cell cycle, it increases p27 accumulation and significantly increases PARP cleavage as a dose-dependent manner. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: PC-3 cells Concentration: 2 μM; 8 μM Incubation Time: 24 hours Result: Induced G1 cell cycle arrest in PC-3 cells. Apoptosis AnalysisCell Line: PC-3 cells Concentration: 2 μM; 8 μM Incubation Time: 24 hours Result: Increased cell apoptosis as a dose-dependent manner. Western Blot AnalysisCell Line: PC-3 cells Concentration: 1 μM; 2 μM; 8 μM Incubation Time: 24 hours Result: Increased PARP cleavage and p27 accumulation as a dose-dependent manner.

In Vivo

UK-101 (intraperitoneal injection; 1-3 mg/kg; twice a week; 3 weeks) decreases tumor volume as a dose-dependent manner, it significantly decreases tumor volume at a dose of 3 mg/kg. Additionally, UK-101-treated mice is suffering less systemic toxicity and the weights of mice treated with UK-101 remains steady over the 3-week treatment period. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Subcutaneously implanted PC-3 cells in 6-week-old male BALB/c athymic nude miceDosage: 1 mg/kg; 3 mg/kg Administration: Intraperitoneal injection; twice a week; 3 weeks Result: Inhibited tumour growth in the prostate cancer mouse xenograft model.

IC50& Target:IC50: 104 nM (LMP2) IC50: 15 μM (immunoproteasome β1c) IC50: 1 μM (immunoproteasome β5)

Specifications

Meccanismi biochimici e fisiologici

UK-101 is a potent and selective immunoproteasome β1i (LMP2) inhibitor with an IC 50 value of 104 nM, displays 144- and 10-fold selectivity over β1c ( IC 50 =15 μM) and β5 subunit ( IC 50 =1 μM), respectivey. UK-101 induces cell apoptosis and can be

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

• Sorrisi canonici: O=C([C@]1(CO1)CO[Si](C)(C)C(C)(C)C)[C@H](CC(C)C)NC([C@H](C)NC(CCCCCC)=O)=O
• Peso molecolare: 484.74

Domande frequenti

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1000313-40-5, the molecular formula is C25H48N2O5Si, and the molecular weight is 484.74 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.