The Gel Shift Assay, also known as the Electrophoretic Mobility Shift Assay (EMSA), is a powerful technique used to study protein-nucleic acid interactions. Initially developed to verify the interaction between transcription factors and promoters, it has been widely adapted to investigate various other protein-DNA and protein-RNA interactions.
Aladdin's UltraBio™ EMSA Precast PAGE Gel is a safe, efficient, and high-performance precast polyacrylamide gel system designed specifically for EMSA experiments.
Product Features:
⁕ Made with glass plates to effectively reduce non-specific protein adsorption, resulting in sharper and clearer protein bands.
⁕ Manufactured using automated gel casting technology to ensure high stability and reproducibility of product quality.
⁕ The gel cassettes are extremely easy to open – simply make a light scratch on one side with a blade.
⁕ Compatible with mainstream mini electrophoresis tanks from Bio-Rad, Invitrogen, Tanon, Junyi East, Beijing Liuyi, and other leading manufacturers.
Basic Information:
Gel Plate Dimensions: Width × Height × Thickness = 100 × 84 × 4.6 mm
Number of Wells: 10 wells, 15 wells
Gel Dimensions: Width × Height × Thickness = 81 × 74 × 1.5 mm
Gel Thickness: 1.5 mm
Maximum Sample Loading: 60 μL, 30 μL
Concentration: 4%, 6%
Packaging: 10 pieces/box
Precast Gel Selection Guide:
E753270
| Precast Gel Concentration
| Number of Wells
| Maximum Sample Loading Volume
| Buffer
| The recommended voltage
|
E753270-A1-10EA
| 4%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B1-10EA
| 4%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
E753270-A2-10EA
| 6%
| 10
| 60 μl
| 0.5xTBE
| 100 V
|
E753270-B2-10EA
| 6%
| 15
| 30 μl
| 0.5xTBE
| 100 V
|
Usage Instructions:
1. Remove the UltraBio™ EMSA Precast PAGE Gel from its packaging, secure it in the electrophoresis tank, and assemble the electrophoresis apparatus.
2. Add 0.5×TBE running buffer to the electrophoresis tank. Fill the inner chamber completely with electrophoresis buffer. Add buffer to the outer chamber to at least 1/3 of the liquid level, but ensure it does not overflow the top of the gel plate. Then, gently remove the comb.
3. Gently pipette up and down in the sample wells to remove any residual gel pieces.
4. Loading Samples: Mix the sample with 4×EMSA/Gel-Shift Loading Buffer (LB4) in a 3:1 ratio and load directly. When loading, ensure the pipette tip does not pierce the gel or insert too deeply, which could cause the gel plate to deform and lead to buffer leakage during subsequent electrophoresis.
5. Connect the power supply and run the gel at a constant voltage (100V). The electrophoresis can be stopped when the bromophenol blue indicator has migrated to the bottom of the gel plate or to the predetermined position in the experiment.
6. After electrophoresis, remove the gel. To open the glass plates, use a knife to cut through the sealing material along the gap between the two glass plates on the side. To remove the gel, make a cut along the glass strips between the gel and the glass plates to prevent adhesion and breakage (please use extreme caution when using a utility knife!).
Gel Disassembly:
1. First, make a simple cut along the side gel (or remove excess gel-sealing material from the sides of the glass plate first);
2. Use a knife to cut through the gel-sealing material along the gap between the glass plate and the glass strip at the side gel (at the arrow), then gently open the glass plate;
3. When removing the gel, make a cut along the glass strips between the gel and the glass strips on both sides to prevent the gel from sticking and breaking during removal.
Compatible Electrophoresis Tanks for UltraBio™ EMSA Precast PAGE Gel:
UltraBio™ EMSA Precast PAGE Gel is compatible with most mini SDS-PAGE electrophoresis tanks, including:
a. Bio-Rad Mini-PROTEAN (II/3 /Tetra System)
b. Hoefer Mighty Small (SE 250/ SE 260/ SE 280)
c. Life Technology Novex Mini-Cell (to be used with Aladdin Custom Baffle 01)
d. Life Mini Gel Tank (to be used with Aladdin Custom Baffle 02)
e. Beijing Liuyi DYCZ-25E, DYCZ-24K, DYCZ-24KS, DYCZ-24KF
f. Junyi East JY-SCZ2+
g. Tanon VE180
or other electrophoresis tanks with a gel plate width of 10 cm.
Application in Life Electrophoresis Tanks: If required, please inform us when ordering this product
Place Baffle No. 01 on the outer side of the gel Place Baffle No. 02 on the inner side of the gel
Application in Bio-Rad Electrophoresis Tanks:
The top of the U-shaped sealing strip in the Bio-Rad Mini-PROTEAN series electrophoresis tanks has a raised structure, while the short glass plate of Aladdin’s UltraBio™ series precast gels has a concave structure—resulting in a flat surface at this location. Before electrophoresis, the sealing strip with the raised structure must be removed and reinstalled in reverse, with the smooth side facing outward, to prevent liquid leakage (as shown in the figure below).
a. Pull out the U-shaped sealing strip (the green part in the figure) from the Bio-Rad electrophoresis tank. Note that the two ends of the sealing strip have protrusions at this point: the side with protrusions is the front side, and the side without protrusions is the back side.
b. Rotate the sealing strip 180 degrees (with the front side facing inward and the back side facing outward) and reinstall it in the electrophoresis tank. Ensure the perimeter of the sealing ring is pressed firmly to prevent liquid leakage.
c. Place the precast gel and proceed with normal electrophoresis operations.
Precautions:
1. Reuse of electrophoresis buffer is not recommended. After electrophoresis, ionic strength, pH and buffering capacity of the buffer will change, which cannot guarantee optimal electrophoresis performance (DNA electrophoresis may result in blurred bands and irregular DNA band migration).
2. For scientific research use only by professional personnel. Not for clinical diagnosis or other improper purposes. Do not store in ordinary residential premises.
3. For your safety and health, please wear personal protective equipment and laboratory clothing during operation.
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