UNI-StabilPLUS - Ready-to-use (RTU)

Cat. No.: U494463

GRADE & PURITY Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Panoramica

Product Characteristics 

UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of pre-diluted, ready-to-use conjugates, minimizing assay errors in dilution. 

Superior stabilization of HRP and AP conjugated antibodies in low as well as high protein dilutions is seen, when using UNI-StabilPLUS. 

When tested with AP conjugated antibody stability is seen as follows: 

• at least 3 years at 2-8 °C 

• at least 2 years at room temperature 

• at least 4 weeks at 37 °C 

When tested with HRP conjugated antibody stability is seen as follows: 

• at least 2 years at 2-8 °C 

• at least 1 years at room temperature 

• at least 2 weeks at 37 °C

UNI-StabilPLUS is recommended for the dilution of antibodies directed against rabbit immunoglobulins unlike HRP-StabilPLUS (cat. no. H494387) and Antibody Enhancer (cat. no. A494276).

Composition & Properties 

UNI-StabilPLUS is a ready-to use buffer that appears as an opaque solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. UNI-StabilPLUS contains neither BSA, nor other material from bovine serum, no azide, mercury or other toxic components.

Working Procedure 

1.Make a series of dilutions of the HRP- or AP conjugated protein in UNI-StabilPLUS in order to determine the optimal dilution. 

2.Run the assay as usual or store the diluted conjugated protein preferably at 2-8 °C.

Tips & Tricks 

• Avoid using phosphate buffers for AP-conjugated antibody assays. We recommend the use of Tris/HCl, Tween as the washing buffer, instead of a PBS buffer which will reduce signal significantly. 

• For extended stability of HRP conjugated antibodies, HRP-StabilPLUS (cat. no. H494387) is recommended. 

Handling & Storage 

• Store solution at 2-8 °C.

Specifications

Specifiche e purezza

Ready-to-use (RTU)

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light

Spedito in

Wet ice

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Proprietà chimiche e fisiche

• Sensibilità: Light sensitive

Application Protocols

Item-specific, validated protocols are not provided in the Product Data. The following are general, non-product-specific workflows for using antibody stabilizers.

• ELISA (general)

  • Prepare coating, blocking, and wash buffers as usual. Dilute detection antibodies in buffer containing a trial level of stabilizer (e.g., 2–5% v/v; not product-specific). Compare signal/background versus control without stabilizer. Verify compatibility with HRP/AP.

• Western blot (general)

  • Prepare primary/secondary antibodies in TBST or PBST supplemented with stabilizer at low percentage to reduce nonspecific adsorption to plastics. Avoid high excipient loads that may affect membrane interactions.

• IHC/IF (general)

  • For working dilutions, include minimal stabilizer necessary to maintain signal over the incubation period. Protect from light when using fluorophore-conjugates. Validate that excipients do not interfere with antigen retrieval or mounting media.

• Flow cytometry (general)

  • Add stabilizer only to antibody master mix, not to cell suspensions, unless validated to avoid effects on cell membranes. Keep samples cold and protected from light.

• Storage of diluted antibodies (general)

  • Short-term (hours–days) at 2–8°C with stabilizer. For longer storage, aliquot and freeze only if SDS/CoA indicates compatibility. Avoid repeated freeze–thaw.

For UNI-StabilPLUS-specific instructions (concentrate/ready-to-use status, recommended ratios), consult the CoA/Spec Sheet.

Biological Roles

Not typically applicable. UNI-StabilPLUS is a laboratory stabilizer mixture rather than a defined biomolecule with a physiological role. If any biological additives are present (e.g., proteins or amino acids), those roles pertain to formulation performance, not endogenous biology. For composition and any biological-source components, consult the CoA/SDS.

Buffer Applications

Relevance

• High relevance. UNI-StabilPLUS is intended for antibody/protein stabilization within buffer systems. Exact composition, pH, and recommended dilution are not specified here; consult CoA/Spec Sheet.

General best practices (literature/general)

• Working dilutions: Prepare antibody dilutions in PBS or TBS containing the stabilizer at the vendor-recommended ratio. If unavailable, pilot a small range (e.g., 1–10% v/v stabilizer) and select the lowest concentration that maintains performance.
• Blocking and background: Stabilizers sometimes reduce nonspecific adsorption. If blocking is still required, use BSA or casein in a separate step; verify compatibility.
• Adsorption control: Use low-bind plastics and include stabilizer to minimize loss of low-abundance antibodies.
• Freeze–thaw mitigation: Combine stabilizer with aliquoting; avoid repeated freeze–thaw where possible. Confirm whether the stabilizer is intended for cold storage or only for short-term, 2–8°C storage.
• Enzyme/fluorophore compatibility: Ensure that excipients do not inhibit HRP/AP or quench fluorophores. Run small controls.

Example workflow (generic)

1. Equilibrate antibody to 2–8°C.
2. Prepare buffer (PBS/TBS) at working ionic strength.
3. Add stabilizer to buffer at trial ratios (e.g., 2%, 5%, 10% v/v; not product-specific), mix gently.
4. Dilute antibody to working concentration; incubate briefly and proceed with assay or store short-term at 2–8°C protected from light.
5. Record performance metrics (signal, background) to optimize the ratio.

Note: For exact usage instructions unique to UNI-StabilPLUS, refer to CoA/SDS.

Green Alternatives

Context

• As an aqueous laboratory stabilizer, UNI-StabilPLUS is generally aligned with low-volatility, nonflammable reagents. However, environmental profile depends on undisclosed formulation specifics.

General greener-practice guidance (literature/general)

• Prefer aqueous, preservative-optimized solutions that minimize hazardous biocides where possible.
• Use smallest effective volumes to reduce waste; adopt aliquoting strategies to extend antibody life without repeated freeze–thaw.
• Choose low-toxicity surfactants and readily biodegradable excipients when alternatives are available (verify composition on CoA/SDS).
• Implement closed, reusable cold-chain packs for shipping/storage to reduce single-use materials.

Comparison table (general, not product-specific)

• Aqueous stabilizer vs. glycerol-heavy mixes
  • Benefits of aqueous: lower viscosity, better pipetting, often lower environmental impact.
  • Tradeoffs: may provide less freeze protection than high-glycerol mixes; composition for UNI-StabilPLUS is not specified.

Disposal

• Treat spent solutions as aqueous laboratory chemical waste unless your SDS indicates otherwise. Avoid drain disposal if preservatives (e.g., azide, isothiazolinones) are present; confirm on SDS.

Pharmaceutical Uses

Not applicable for therapeutic or clinical use. UNI-StabilPLUS is designated For research use only and is intended for laboratory stabilization of antibody/protein reagents. No pharmacopeial status, excipient listing, or GMP manufacturing details are provided. Do not use in human or veterinary applications.

Physical Properties

Item-specific values

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, pH, conductivity: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet (stabilizers of this type are commonly supplied ready-to-use in aqueous media, but composition for UNI-StabilPLUS is not disclosed).

Literature/general expectations for protein stabilizer solutions (not product-specific)

• Physical state: Typically clear, aqueous solutions.
• pH range: Often formulated near neutral pH to maintain antibody integrity (commonly ~pH 6.8–8.0; literature/general).
• Osmolality: Adjusted with salts or polyols to reduce stress-induced aggregation (literature/general).
• Freeze-thaw behavior: Stabilizers are often intended to minimize freeze–thaw damage when combined with protein; however, exact cryoprotectant content for UNI-StabilPLUS is not specified.

Notes

• Do not infer composition or performance from general properties. For exact specifications (e.g., pH, conductivity, viscosity, UV absorbance background), consult the product’s CoA/Spec Sheet.

Quality and Grades

Item-specific grade/purity

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

What quality typically means for antibody stabilizers (general context)

• Filtration/bioburden: High-quality stabilizers are commonly sterile-filtered (e.g., 0.2 µm) and produced under controlled bioburden to reduce contamination risk (literature/general).
• Background absorbance/fluorescence: Low UV/Vis background is important for ELISA and spectroscopic assays; low autofluorescence aids IF/flow applications (literature/general).
• Lot-to-lot consistency: Tight control of excipient levels and pH ensures reproducible antibody performance.
• Additives/preservatives: Presence, identity, and concentration strongly impact compatibility with enzyme conjugates (HRP/AP) and downstream assays. Confirm via CoA if critical.

How to use CoA/Spec Sheet effectively

• Verify pH, conductivity, sterility/bioburden status, endotoxin limits, and any preservative identity.
• Check any stated compatibility (e.g., suitability for long-term storage vs. working-dilution stabilization).
• Confirm appearance and acceptance criteria to support incoming QC.

Note: In the absence of item-specific grade details above, rely on the SDS/CoA for procurement qualification.

Reaction and Applications

Scope for this product type

• This product is intended as a stabilizing medium for antibodies and proteins; it is not a reagent for organic synthesis or named reactions. Therefore, traditional synthetic reaction applications (e.g., cross-couplings, reductions) do not apply.

Laboratory use cases (literature/general, not product-specific)

• Antibody storage stabilization: Helps maintain conformation, reduce aggregation, and mitigate adsorption to vessel walls during refrigerated storage and short-term bench handling.
• Working dilution buffer component: When preparing primary/secondary antibody dilutions for ELISA, WB, IHC, IF, or flow cytometry, stabilizers may improve signal consistency by preserving active binding sites.
• Freeze–thaw protection: Inclusion with aliquoted antibody stocks can lessen activity losses from limited freeze–thaw cycles. Exact cryoprotectant content for UNI-StabilPLUS is not specified—verify before freezing.
• Low nonspecific binding: Some stabilizers include blocking moieties to reduce background. Composition for UNI-StabilPLUS is not disclosed; evaluate empirically for your assay.

Practical tips

• Run a small matrix of dilution factors and incubation times with and without stabilizer to quantify benefits in your specific assay.
• Check compatibility with enzyme conjugates (HRP/AP) and fluorescent dyes; quenching or inhibition can occur with certain excipients.
• If downstream mass spectrometry or protein labeling is planned, confirm that the stabilizer does not contain reactive amines or interfering detergents (composition for this item is not specified).

Reaction Conditions

Not applicable to chemical synthesis. For laboratory workflows involving antibodies and proteins, conditions pertain to storage and assay buffers rather than chemical reactions.

General handling conditions for protein stabilization (literature/general)

• Temperature: Keep samples on ice or at 2–8°C during setup to limit proteolysis/denaturation.
• Mixing: Gentle inversion or slow pipetting to avoid shear; avoid vortexing monoclonal IgGs.
• Light: Protect fluorophore-conjugated antibodies from light; this product itself should be protected from light per Product Data.
• Ionic strength and pH: Maintain within the validated range for your antibody clone.

Specific temperatures, times, and compositions for UNI-StabilPLUS are not provided; consult the CoA/Spec Sheet for any product-specific instructions.

Safety and Handling

Item-specific safety data

• GHS classification, hazard statements, signal word, pictograms: Not specified for this item; refer to the SDS for authoritative information.
• Storage conditions (from Product Data): Store at 2–8°C, protected from light. Shipped on wet ice.

General laboratory precautions (not product-specific)

• PPE: Wear lab coat, safety glasses, and appropriate chemical-resistant gloves. Use in a well-ventilated area or biological safety cabinet if handling proteinaceous materials.
• Incompatibilities: Avoid mixing with strong oxidizers or reactive chemicals until composition is reviewed. Do not autoclave unless explicitly permitted in the SDS/CoA; many stabilizers are heat-sensitive.
• Preservatives: Protein stabilizers sometimes contain biocides (e.g., isothiazolinones or azide) to control bioburden (literature/general). Presence or absence in UNI-StabilPLUS is not specified—verify before use with azide-sensitive systems (e.g., HRP) or copper plumbing.
• First aid (general): In case of skin/eye contact, rinse with water for 15 minutes; if inhaled, move to fresh air; if ingested, rinse mouth—seek medical attention as needed. Follow your institution’s chemical hygiene plan.
• Spills and disposal: Absorb small spills with inert material; dispose according to local regulations. Treat used solutions as laboratory chemical waste.

Always consult the product SDS for definitive hazard and handling instructions.

Solvent Selection

Applicability

• UNI-StabilPLUS is supplied as an aqueous stabilizer for protein reagents (functional category). Organic solvent selection is not typically relevant. Focus should be on buffer system compatibility with your antibody/reagent.

General guidance (literature/general)

• Compatibility: Such stabilizers are usually mixed with PBS or TBS systems. Confirm ionic strength and pH interactions before large-scale use.
• Detergents/surfactants: If your assay is sensitive to surfactants (e.g., membrane protein work or MS), verify whether the stabilizer contains any; composition for this item is not specified.
• Additive dilution: When preparing working solutions, maintain the vendor-recommended ratio to avoid excessive viscosity or salt that could impair binding kinetics. Specific ratios for UNI-StabilPLUS are not provided; perform small pilots.

Comparison (general)

• Pure buffer (PBS/TBS) vs. stabilizer-supplemented buffer: Stabilizers can reduce aggregation, adsorption to plastics, and freeze–thaw damage, at the cost of introducing additional components that may interact with certain assays. Choose based on assay tolerance to excipients.

Storage and Reconstitution

Item-specific instructions (from Product Data)

• Storage: Store at 2–8°C, protected from light.
• Shipping: Shipped on wet ice.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet (product may be supplied ready-to-use; verify before dilution).

General guidance (not product-specific)

• If supplied as a concentrate, equilibrate to 2–8°C before opening to minimize condensation. Dilute with sterile PBS/TBS as directed by the manufacturer using sterile technique.
• Avoid microbial contamination: Use sterile tips/tubes; consider aliquoting on first use. Do not autoclave unless expressly allowed.
• Light protection: Store in amber containers or wrap in foil, especially if the formulation includes light-sensitive components.
• Stability monitoring: Inspect visually for turbidity, precipitate, or color change. Check pH if performance drifts. Discard if contamination or instability is suspected.

Freeze–thaw

• Only freeze if the CoA/SDS indicates the formulation tolerates freezing. Some stabilizers contain components that crystallize or phase-separate upon freezing; others include cryoprotectants by design. For UNI-StabilPLUS, this is not specified—use refrigerated storage as directed.

Structure and Identity

Brief overview: UNI-StabilPLUS is a proprietary laboratory stabilizer formulated for antibodies and protein reagents. Item-specific structural identifiers are not disclosed.

• Item-specific identifiers (from Product Data)

  • Chemical name: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general context)

  • As a stabilization reagent for proteins, such formulations are typically multi-component aqueous mixtures (literature/general). They may include buffering agents, osmolytes, salts, and protein-friendly excipients designed to limit aggregation, denaturation, and surface adsorption. Specific constituents for UNI-StabilPLUS are not provided here.

• 2D structure (descriptive)

  • Not applicable to a proprietary multi-component mixture with undisclosed composition.

• Category

  • Listed under: 全部 / 可售 / 抗体 (catalog category). Despite the category path, this product is a stabilizer for antibody handling rather than an antibody itself (general catalog context).

Synthetic Utility

Not applicable. UNI-StabilPLUS is not a synthetic building block or reagent for chemical transformations. It serves as a formulation aid for biomolecule handling. For synthetic chemistry needs (e.g., coupling reagents, solvents), refer to appropriate product categories.

Target Specificity

This product is a stabilizer, not an antibody. Therefore, antigen target, species reactivity, clone, and isotype are not applicable. If you require an antibody validated for specific targets, please refer to our antibody catalog.

Domande frequenti

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.