Uricase (UAO), CAS No.9002-12-4

CAS: 9002-12-4 Cat. No.: R1506897 Numero EC: 232-655-5 PubChem CID: 14228836

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥80%(SDS-PAGE), expressed in E.coli

Accession #

G8ZWT4

Expression system

E. coli

Bioactivity

8-12 U/mg enzyme powder

Panoramica

This product is a recombinantly expressed urate oxidase (UAO) intended for in vitro research use.

Assay Procedure

Principle: 

The disappearance of uric acid is measured at 290nm by spectrophotometry.

Unit definition:

One unit will oxidase one micromole of uric acid per minute at pH8.5 at 25℃

Reagents:

A. Uric acid solution:0.001% [Dilute the stock solution (0.01%) to 10-fold volume with 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0] (should be prepared fresh)

Stock solution:10.0mg uric acid/100ml of above buffer (Store at 0~5℃)

B. KOH solution: 20%

C. Enzyme diluent: 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0.

Procedure:

1. Prepare the following reaction mixture in a test tube and equilibrate at 37℃ for about 5 minutes.2.0ml Uric acid solution (Reagent A) 0.5ml Distilled water
2. Add 0.5ml of the enzyme solution* and mix by gentle inversion.
3. After exactly 5 minutes at 37℃, add 0.2ml of 20% KOH solution (B) to stop the reaction and measure the optical density at 290nm against water (OD test).At the same time, prepare the blank by first mixing the reaction mixture with 0.2ml KOH solution after 5min-incubation at 37℃, followed by the addition of the enzyme solution (OD Blank).

*:Dissolve the enzyme preparation in ice-cold enzyme diluent (C) and dilute to 0.01~0.02U/ml with the same buffer and store on ice.

Calculation:

Activity can be calculated by using the following formula:

Vt: Total volume (3.2ml)

Vs: Sample volume (0.5ml)

12.2: Millimolar extinction coefficient of uric acid (cm²/μmol)

t: Reaction time (5 minutes)

1.0: Light path length (cm)

df: Dilution factor

C: Enzyme concentration in dissolution (c mg/ml)

Specifications

Product Name

Uricase (UAO), CAS No.9002-12-4

Sinonimi

Urate: oxygen oxoreductase | factor independent urate hydroxylase | urate oxidase | uric acid oxidase | uricase | uricase II

Grado

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifiche e purezza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥80%(SDS-PAGE),expressed in E.coli

Bioattività

8-12 U/mg enzyme powder

Numero d'ordine

G8ZWT4

CAS

9002-12-4

Numero della Commissione Enzimi

EC 1.7.3.3

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

expressed in E.coli

Ricostituzione

Reconstitute in ice-cold 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0.

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ long term (12 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definizione dell'unità

One unit will oxidase one micromole of uric acid per minute at pH8.5 at 25°C.

Riferimenti

1. Zheng Xinyu, Yang Yang, Gao Feng, Li Huihui, Yang Weikang, Guo Dong-Yu, Chen Shengming, Pan Qinhe.  (2022)  Enzyme-free fluorescence determination of uric acid by combining CdTe quantum dots with metal–organic framework for signal amplification.  MICROCHIMICA ACTA,  189  (11): (1-9).  [PMID:36318325] [10.1007/s00604-022-05535-8]

2. Fuad Abduro Bushira, Shimeles Addisu Kitte, Yong Wang, Haijuan Li, Ping Wang, Yongdong Jin.  (2021)  Plasmon-Boosted Cu-Doped TiO2 Oxygen Vacancy-Rich Luminol Electrochemiluminescence for Highly Sensitive Detection of Alkaline Phosphatase.  ANALYTICAL CHEMISTRY,      [PMID:34743510] [10.1021/acs.analchem.1c03842]

3. Guojun Cao, Can Wu, Yong Tang, Chidan Wan.  (2019)  Ultrasmall HKUST-1 nanoparticles decorated graphite nanosheets for highly sensitive electrochemical sensing of DNA damage biomarker 8-hydroxy-2’-deoxyguanosine.  ANALYTICA CHIMICA ACTA,      [PMID:30851856] [10.1016/j.aca.2019.01.031]

4. Aijin Fang, Qiongqiong Wu, Qiujun Lu, Hongyu Chen, Haitao Li, Meiling Liu, Youyu Zhang, Shouzhuo Yao.  (2016)  Upconversion ratiometric fluorescence and colorimetric dual-readout assay for uric acid.  BIOSENSORS & BIOELECTRONICS,      [PMID:27471157] [10.1016/j.bios.2016.07.055]

5. Yuanting Xie, Kaidi Li, Jing Liu, Ying Zhou, Chuangjie Zhang, Yan Yu, Jianlong Wang, Lei Su, Xueji Zhang.  (2024)  A smart lab on a wearable microneedle patch with convolutional neural network-enhanced colorimetry for early warning of syndrome of inappropriate antidiuretic hormone secretion.  Aggregate,      [PMID:] [10.1002/agt2.671]

6. Zehua Guo, Yiren Cao, Liuyin Fan, Weiwen Liu, Li Wei, Yixin Ma, Jicun Ren, Qiang Zhang, Chengxi Cao.  (2024)  A temperature-independent model of dual calibration standards for onsite and point-of-care quantification analyses via electrophoresis titration chip.  ANALYTICA CHIMICA ACTA,      [PMID:38245206] [10.1016/j.aca.2024.342207]

7. Yanshu Xiang, Yongjie Zhao, Yifan Jiang, Jiarui Feng, Xiaoyi Zhang, Wei Su, Yingjie Zhou, Peng Wei, Sze Shin Low, Hao Nan Li.  (2024)  Battery-Free and Multifunctional Microfluidic Janus Wound Dressing with Biofluid Management, Multi-Indicator Monitoring, and Antibacterial Properties.  ACS Applied Materials & Interfaces,      [PMID:39264683] [10.1021/acsami.4c09891]

8. Yunfeng Bai, Xiaoxuan Fan, Guoning Chen, Zhilong Zhao.  (2024)  Efficient and fast detection of uric acid based on a colorimetric sensing method.  Analytical Methods,      [PMID:39157953] [10.1039/D4AY01053A]

9. Fangbing Wang, Jing Ren, Qiwen Peng, Hongyi Sun, Qiankun Zeng, Yongheng Zhang, Guoyue Shi, Min Zhang.  (2024)  Janus Separation-Sensing Membrane Hosted with Enzyme@MOF Nanoreactor for Real-Time Blood Sensing.  ANALYTICAL CHEMISTRY,      [PMID:39264829] [10.1021/acs.analchem.4c03285]

10. Yi-Kun Wang, Ya-Jun Chen, Yao Pan, Yu Hai-Yin.  (2024)  Prussian Blue and Polyvinylpyrrolidone-Embedded Hollow Fiber Gradient Polysulfone Membrane for Uric Acid Biosensor Application.  JOURNAL OF APPLIED POLYMER SCIENCE,      [PMID:] [10.1002/app.56649]

11. Chunjiao Qi, Jintao Chen, Yuhui Shang, Yu Yang, Kangyan Wang, Jinyang Chen.  (2024)  Target-Triggered Ultrafast Chondroitin Gelation Enabled Power-Free and Point-of-Care Bioassays.  ANALYTICAL CHEMISTRY,      [PMID:39436985] [10.1021/acs.analchem.4c04130]

12. Chenlong He, Huawei Liu, Ming Yin, Jing Chen, Wensi Huang, Han Zhou, Shengming Wu, Yilong Wang.  (2025)  UOx@HMnO2 Biozyme-Nanozyme Driven Electrochemical Platform for Specific Uric Acid Bioassays.  ANALYST,      [PMID:40035522] [10.1039/D4AN01512F]

13. Ziting Bao, Sheng Lu, Duo Zhang, Guanyu Wang, Xiaolin Cui, Guozhen Liu.  (2024)  Wearable Microneedle Patch for Colorimetric Detection of Multiple Signature Biomarkers in vivo Toward Diabetic Diagnosis.  Advanced Healthcare Materials,      [PMID:38353398] [10.1002/adhm.202303511]

14. Xiwang Hou, Tianhao Chen, Chunyan Wang, Fanyu Liu, Minggang Zhao, Ye Ma.  (2025)  Analyte mediated transformation of metasurface: A dual-mode plasmonic sensing platform for uric acid.  TALANTA,      [PMID:41166825] [10.1016/j.talanta.2025.129024]

15. Ruoxuan Jiang, Lei Pan, Fan Yang, Shudong Zhang, Dan Lin, Changlong Jiang.  (2025)  MOF-on-MOF multi-enzyme cascade sensor for visual monitoring of uric acid in body fluids.  TALANTA,      [PMID:41240652] [10.1016/j.talanta.2025.129115]

16. Lanlan Wu, Xiaochun Deng, Jiyang Liu.  (2026)  Electrochemiluminescence Sensor for Uric Acid Detection Using Natural Enzyme‐Confined Nanozyme Cascade.  LUMINESCENCE,  41  (7): (e70592).  [PMID:42489283] [10.1002/bio.70592]

17. Sui Zhou, Wen-Qian Zhang, Yong Li, Rong-Fen Gao, Jia-Yi Ma, Kai Cheng, Jin-Xuan Fan, Jie Yang, Ling-Li Dong, Bo Liu.  (2026)  Multimetal-Doped Nanoenzymes Reprogram Macrophages for Immunotherapy of Gouty Arthritis.  Advanced Healthcare Materials,      [PMID:42396921] [10.1002/adhm.71410]

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥80% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9002-12-4.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.