USH1C/Harmonin Mouse mAb - Anticorpo primario, specific to USH1C/Harmonin, Mouse IgG1

Cat. No.: Ab133668

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Mouse, Umano

Isotype

Mouse IgG1

Clone Number

K06328_5A8

Conjugation

Unconjugated

Panoramica

Mouse anti Human USH1C/Harmonin Antibody, Monoclonal (K06328_5A8), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/1000
Protein Function:
May be involved in protein-protein interaction.

Specifications

Product Name

USH1C/Harmonin Mouse mAb - Anticorpo primario, specific to USH1C/Harmonin, Mouse IgG1

Sinonimi

AIE 75antibody | AIE75 antibody | Antigen NY CO 38/NY CO 37 antibody | Antigen NY-CO-38/NY-CO-37 antibody | Autoimmune enteropathy related antigen AIE 75 antibody | Autoimmune enteropathy related antigen AIE75 antibody | Autoimmune enteropathy-related ant

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

USH1C/Harmonin

Immunogeno

Recombinant protein of human USH1C/Harmonin (AA 301-550).

Controllo positivo

WB: Mouse kidney lysates. IHC: Human small intestine tissue.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: USH1C/Harmonin Mouse mAb (Ab133668)

USH1C/Harmonin Mouse mAb (Ab133668) - Western Blot
All lanes: USH1C/Harmonin Mouse mAb (Ab133668) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 67 kDa
Exposure time: 100.0 s

IHC: USH1C/Harmonin Mouse mAb (Ab133668)

USH1C/Harmonin Mouse mAb (Ab133668) - IHC
IHC analysis of small intestine tissue by USH1C/Harmonin Mouse mAb (Ab133668).IHC was performed using sections of the formalin-fixed paraffin-embedded small intestine tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with USH1C/Harmonin Mouse mAb (Ab133668) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Glandular cells are positively stained at the cytoplasm and membrane.

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/1000

Obiettivi associati (umani)

USH1C Tbio Harmonin (0 Activities)

Discover Target: USH1C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (USH1C/Harmonin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.