WAY-324944 - 10mM in DMSO , CAS No.380580-42-7

CAS: 380580-42-7 Cat. No.: W423756 Formula: C₁₈H₁₉N₃O₂S Peso molecolare: 341.43

QUALITÀ & PUREZZA 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

anti-viral agent

Specifiche

Specifiche e purezza

10mM in DMSO

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

Sorrisi canonici

CC(=C)CN1C=NC2=C(SC3=C2C=C4COC(C)(C)CC4=N3)C1=O

Peso molecolare

341.43

Application Protocols

• Item-specific validated applications: Not specified for this item.

• General protocol (small-molecule screening stock and assay dilution)

  1. Equilibrate vial to room temperature in a desiccator before opening to avoid moisture condensation.
  2. In a fume hood, dissolve compound in anhydrous DMSO to prepare a 10 mM master stock (adjust if solubility allows). Vortex thoroughly; brief sonication may help.
  3. If required, sterile-filter the stock through a 0.22 µm PTFE membrane into a labeled, amber polypropylene tube.
  4. Aliquot into single-use volumes (e.g., 10–50 µL) to avoid repeated freeze–thaw. Store at -80°C.
  5. For assays, add stock to pre-warmed buffer/media with mixing to achieve the desired final concentration. Keep final DMSO consistent across controls and treatments (e.g., 0.1% v/v).
  6. Include vehicle-only and positive/negative controls appropriate to the assay readout. Incubate per assay SOP.
  7. Inspect wells for precipitation; if observed, reduce concentration, increase mixing time, or adjust vehicle.

• Notes

  • Record batch/lot, stock concentration, and dispense method. Confirm working concentrations by LC–MS or UV where quantitative accuracy is essential.
  • Dispose of DMSO-containing waste per institutional guidelines.

Biological Roles

• Item-specific

  • No biological target, pathway, or mechanism is specified for WAY-324944 in the provided product data.

• General context for small-molecule research tools (literature best practices)

  • Discovery compounds are often evaluated in pathway-reporter assays, enzymatic panels, or phenotypic screens to uncover activity profiles. When the target is unknown, chemoproteomics or broad profiling (kinases, GPCRs, safety panels) may be used to build an MoA hypothesis.
  • Orthogonal assays and structural analogs help link activity to structure (SAR) and exclude assay artifacts (e.g., redox cycling, aggregation, fluorescence interference).
  • ADME liabilities (solubility, permeability, metabolic stability) can confound biological readouts; consider running microsomal stability, plasma protein binding, and permeability (PAMPA/Caco-2) early if translational in vitro work is planned.
  • Cytotoxicity evaluation in the relevant cell type helps establish testing windows and avoid misinterpreting stress responses as specific pathway modulation.

• Important limitations

  • Without item-specific target information, no claims can be made regarding receptor/enzymatic specificity or pathway modulation. Users should consult primary literature (by CAS 380580-42-7, if available) and generate in-house confirmation data before drawing mechanistic conclusions.

Buffer Applications

This product is not a buffering reagent. No acid/base pairs, pKa values, or buffer recipes are provided or implied.

• Practical guidance (general for small-molecule assays)
  • Prepare concentrated DMSO stocks and dilute into assay buffer (e.g., HEPES, PBS) with vigorous mixing to avoid precipitation.
  • Maintain constant vehicle concentration across conditions (commonly 0.1–0.5% DMSO) to control for solvent effects.
  • Include detergent (e.g., 0.01–0.05% Tween 20) where compatible to mitigate colloidal aggregation.
  • Filter buffers (0.22 µm) and equilibrate to assay temperature to improve reproducibility.

No buffer capacity or pH range is specified for this item; refer to your assay system’s validated buffer conditions.

Green Alternatives

Green chemistry considerations are generally aligned to solvent/reagent selection. Because WAY-324944 is a research library compound (not a process solvent or common reagent), “green alternative” discussions focus on formulation and handling rather than replacing the compound itself.

• Not specific to this item

  • There are no greener substitutes for the identity of a screening compound without altering study objectives. If your goal is pathway interrogation, consider chemotype alternatives known for better stability or safety, but this requires target-specific knowledge not provided here.

• Greener formulation practices (general)

  • Prefer aqueous assay buffers with minimal DMSO (≤0.1–0.5% v/v) after preparing a concentrated DMSO stock.
  • Use ethanol or water/ethanol mixtures where compatible as a lower-toxicity vehicle versus DMF/NMP.
  • Minimize solvent volumes via high-concentration stocks, acoustic dispensing, and microscale assays.
  • Employ closed microplates and cold storage to reduce solvent loss and emissions.

• Waste minimization

  • Consolidate DMSO and halogenated/organic waste per local regulations; avoid mixing aqueous and organic streams.
  • Use in-plate serial dilutions to limit consumables and solvent usage.

No item-specific green certifications or alternatives are provided for this product.

Pharmaceutical Uses

• Item-specific

  • This product is designated For research use only. It is not an excipient or API and is not intended for human or veterinary use. No pharmacopeial monograph or compendial status is provided.

• General formulation notes for research compounds (non-clinical)

  • For in vitro work: Prepare DMSO stocks (e.g., 10–50 mM) and dilute into compatible media immediately before use.
  • For exploratory in vivo research (if institutionally approved): Vehicle selection typically considers solubility and tolerability (e.g., 10–20% PEG400, 10% Solutol HS 15 or Cremophor EL, 10% ethanol, balance saline/buffer). These are general examples only and must be optimized and approved per protocol; none are item-specific recommendations.
  • Characterize stability (freeze–thaw, light, pH) before formulating.

No clinical or therapeutic claims are made or implied for WAY-324944.

Physical Properties

• Item-specific (from Product Data)

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Physical constants (literature/computed; none provided)

  • Melting point, boiling point, density, refractive index, logP/logD, pKa, UV–Vis cutoff: Not specified for this item; refer to CoA/Spec Sheet or primary literature for CAS 380580-42-7.

• Solubility and formulation guidance (general/literature practice for small molecules)

  • Primary solvent for stock solutions: DMSO is typically preferred for discovery compounds; prepare concentrated stocks (e.g., 10–50 mM) and dilute into assay buffer just before use to a final DMSO ≤0.1–0.5% v/v to minimize vehicle effects.
  • Alternative solvents: DMF, ethanol, or isopropanol may be used if DMSO is unsuitable; confirm by a small-scale solubility screen (visual clarity, absence of precipitation upon dilution).
  • Aqueous media: If water solubility is limited, use co-solvent systems (e.g., 2–10% DMSO with 0.01–0.1% non-ionic surfactant such as Pluronic F-127 or Tween 80) as allowed by the assay.
  • Stability checks: Verify solution stability (no color change/precipitate) across the planned temperature/time window; use LC–UV/MS to confirm concentration where critical.

Note: Do not treat the above as product specifications; they are general best practices for small-molecule library members.

Quality and Grades

• Item-specific (from Product Data)

  • Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
  • Research Use: For research use only (RUO). Not for diagnostic or therapeutic use.

• What this means for your workflows

  • RUO materials are intended for discovery, screening, and method development. Batch-specific analytical details (assay purity, residual solvents, water content, identity confirmation by NMR/MS/HRMS, and chromatographic trace) are provided on the Certificate of Analysis (CoA) when available.
  • If UV transparency or trace metal limits are critical (e.g., for bioassay interference or catalysis studies), verify with the lot-specific CoA. Not specified for this item; refer to CoA/Spec Sheet.
  • Stabilizers/inhibitors: Not specified for this item; refer to CoA/Spec Sheet. If present, they can affect certain assays; consider additional purification if necessary.

• Recommended incoming QC (general best practice)

  • Confirm identity by LC–MS and purity by HPLC/UPLC upon receipt if the compound will be used in quantitative biology.
  • Establish a reference stock (e.g., 10 mM in DMSO) and verify concentration by UV or quantitative NMR where feasible.
  • Record storage history and aliquot to reduce freeze–thaw exposure.

Reaction and Applications

• Item-specific

  • Manufacturer Applications: Not specified in the product data.

• Applicability

  • WAY-324944 is offered as part of a small-molecule/compound library (discovery research). It is not primarily intended as a reagent or solvent for synthetic reactions.

• Research usage patterns (general for library members)

  • Tool compound work: Evaluate in biochemical or cell-based assays, establish dose–response curves, and determine preliminary potency metrics (e.g., IC50/EC50) where relevant to your study design. Without a known target provided here, users typically start with broad phenotypic assays or pathway-reporter systems.
  • Orthogonal confirmation: Run at least two assay formats (e.g., biochemical vs. cellular reporter) to mitigate assay artifacts and DMSO effects.
  • Counter-screens: Include redox/cell health panels (e.g., resazurin, CellTiter) and promiscuity filters (e.g., detergent-sensitive aggregation) to identify false positives.

• Assay interferences (general)

  • Colored/fluorescent compounds or redox-active scaffolds can interfere with optical assays. Run inner-filter and autofluorescence controls when using fluorescence or absorbance readouts.
  • Detergent (0.01–0.05% Triton X-100 or Tween 20) can mitigate colloidal aggregation where compatible with assay.

For synthetic transformations or named reactions: not applicable for this item given no structural or functional group information is provided.

Reaction Conditions

Not applicable. WAY-324944 is not supplied as a reagent for chemical synthesis, and no structural information is provided to suggest participation in specific reactions.

• Practical guidance (general for handling in research assays)
  • Stock preparation: Dissolve in an appropriate solvent (commonly DMSO) at 10–50 mM. Vortex and, if needed, sonicate briefly to aid dissolution.
  • Dilution: Add the organic stock to the aqueous assay buffer with vigorous mixing; maintain consistent vehicle concentration across wells.
  • Stability: Keep solutions cold and protected from light during setup; discard if precipitation or discoloration occurs. Verify concentration by LC–UV/MS for quantitative studies.
  • Plate-based work: Use polypropylene plates for DMSO stocks; seal plates to minimize evaporation. For acoustic dispensing, confirm compound acoustic compatibility.

For any true chemical reaction conditions (solvent, temperature, catalysts, yields), consult literature only after the compound’s structure is confirmed. None are provided for this item.

Safety and Handling

• Item-specific (from Product Data)

  • GHS Classification: Not specified for this item; refer to SDS.
  • Signal Word: Not specified for this item; refer to SDS.
  • H-Statements/Pictograms: Not specified for this item; refer to SDS.
  • Storage Conditions: Store at -80°C
  • Shipped In: Dry ice packs + Cold packs
  • Research Use: For research use only

• General laboratory precautions (applicable to small-molecule research compounds; defer to SDS for authoritative guidance)

  • PPE: Lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., disposable nitrile). Handle powders/solids in a certified chemical fume hood; avoid inhalation of dust/aerosols.
  • Avoid: Contact with skin/eyes and ingestion. Prevent release to the environment.
  • Incompatibilities: Unknown for this specific item; as a precaution, segregate from strong oxidizers/acids/bases until specific compatibility is known.
  • First aid (overview): If inhaled, move to fresh air; if on skin, wash with soap and water; if in eyes, rinse cautiously with water for several minutes; if ingested, rinse mouth. Seek medical attention in all cases. Provide SDS to responders.
  • Spill response: Avoid dust; cover with an inert absorbent, collect mechanically, and dispose according to institutional and local regulations.
  • Stability considerations: Many discovery compounds are moisture-, light-, and/or temperature-sensitive. Minimize freeze–thaw cycles by aliquoting; protect from light until stability is confirmed.

Always consult the current SDS for WAY-324944 for specific hazard statements and emergency measures.

Solvent Selection

This product is a small-molecule research compound; no solvent preferences are specified for the item. The following guidance reflects general practice for dissolution and assay use.

• Item-specific

  • Solubility data: Not specified for this item; refer to CoA/Spec Sheet.

• General selection strategy (literature/practice)

  • Primary solvent: DMSO (high polarity, wide solubilizing power, miscible with water and most organics). Typical stock: 10–50 mM.
  • Secondary options: DMF or NMP (strong solvents, hygroscopic), ethanol or isopropanol (less polar, lower cytotoxicity than DMF/NMP), acetonitrile (volatile, aprotic).
  • Aqueous dilution: Add concentrated organic stock slowly to vigorously mixed buffer to avoid precipitation. Keep final DMSO ≤0.1–0.5% v/v for cell-based assays when possible.
  • pH effects: Weak acids/bases may show pH-dependent solubility; if structure is known internally, choose buffer pH to favor ionized form for aqueous work, or neutral form for permeability studies.
  • Additives: For difficult compounds, consider 0.01–0.1% non-ionic surfactant (Pluronic F‑127, Tween 80) or complexation (2‑hydroxypropyl‑β‑cyclodextrin) as assay-permitted.

• Practical tips

  • Perform a micro-solubility screen (DMSO, ethanol, DMF, aqueous + 2–5% DMSO) and inspect clarity pre/post-dilution.
  • Filter solutions (0.22 µm PTFE) when compatible with assay to remove particulates.
  • Document solvent and vehicle composition with results to ensure reproducibility.

Storage and Reconstitution

• Item-specific (from Product Data)

  • Storage Conditions: Store at -80°C
  • Shipped In: Dry ice packs + Cold packs
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.

• Reconstitution (general guidance for small molecules)

  • Preferred solvent: Anhydrous DMSO for primary stock solutions unless solubility dictates otherwise.
  • Stock concentration: Commonly 10–50 mM; determine the highest fully soluble concentration empirically. Label container with compound ID, lot, concentration, solvent, and date.
  • Aliquoting: Prepare single-use aliquots to minimize freeze–thaw. Store aliquots tightly sealed in inert, DMSO-compatible vials or plates (polypropylene) at -80°C.

• Handling and stability

  • Warm to room temperature before opening to avoid moisture ingress; promptly reseal under dry air or nitrogen if repeated access is needed.
  • Protect from light until photostability is known. Avoid repeated temperature cycling; use cold blocks for on-bench work.
  • Aqueous working solutions are generally less stable; prepare fresh immediately before experiments and discard after use unless stability is verified analytically.

• Shelf-life

  • Not specified for this item; refer to CoA/Spec Sheet. For long-term projects, periodically re-check identity and purity by LC–MS/HPLC.

Always consult the SDS and CoA for any lot-specific guidance on storage stability, hygroscopicity, or light sensitivity.

Structure and Identity

Summary for product WAY-324944 (SKU W423756).

• Item-specific (from Product Data)

  • Product Name: WAY-324944
  • SKU: W423756
  • CAS: 380580-42-7
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features

  • Not specified for this item; refer to CoA/Spec Sheet for structure, functional groups, and any stereochemical information.

• 2D structure (verbal description)

  • Not available in the provided data. Without a structure or SMILES, ring systems, heteroatoms, and substitution pattern cannot be described. Please consult the CoA/SDS or contact Technical Support for the current structural file (SDF/MOL) if required for cheminformatics or registration.

Synthetic Utility

WAY-324944 is offered as a research compound for biological studies, not as a synthetic reagent or building block.

• Item-specific

  • Functional groups and reactivity: Not specified for this item; refer to CoA/Spec Sheet.

• General notes

  • Without structural information (SMILES/InChI), no guidance can be given on derivatization, protecting group strategies, or participation in named reactions.
  • If synthetic elaboration is desired (e.g., biotinylation, photoaffinity tagging), obtain the structure and assess handle availability (amines, phenols, carboxylates, halides). Validate that modifications do not abrogate activity via parallel testing.
  • For analytical support, LC–MS/MS method development (precursor/product m/z, retention factor) should be established from authentic standards or provided spectra.

As a result, this section is not typically applicable for this product class; prioritize assay development, stability, and solubility characterization instead of synthetic transformations.

Target Specificity

• Item-specific

  • No target, binding partner, or selectivity profile is provided in the Product Data for WAY-324944.

• General guidance

  • If the compound is evaluated as a potential modulator, determine specificity by testing against related protein families or pathway panels, using orthogonal assay formats to minimize detection bias.
  • Consider broad profiling (e.g., kinase or GPCR panels, if relevant) to identify off-targets and safety alerts early.

No claims of target specificity can be made for this product without supporting data; refer to CoA/Spec Sheet and primary literature (CAS 380580-42-7) if available.

Domande frequenti

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 380580-42-7, the molecular formula is C18H19N3O2S, and the molecular weight is 341.43 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.