Western Blot Fast Stripping Buffer - BioReagent, ready-to-use, suitable for electrophoresis, for western blot

Cat. No.: W1491287

GRADE & PURITY BioReagent, Suitable for electrophoresis, Ready-to-use, for Western blot

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Electrophoresis, WB

Panoramica

  Eliminates the need to waste precious samples on repeated gel electrophoresis for detecting different primary antibodies. Simply treat the membrane with this stripping buffer to remove the first primary antibody. This process typically takes 10–20 minutes, depending on the amount of antibody bound to the membrane. After stripping, the membrane can be reprobed with a new primary antibody.

Key Features

1. Saves samples: The same membrane with identical samples can be used for multiple detections.

2. Time-efficient: No need to rerun gel electrophoresis.

3. Highly effective: Superior stripping performance compared to homemade buffers.

4. Gentle formulation: Minimal damage to target proteins on the membrane.

5. Odorless: No pungent odor, more environmentally friendly.

Protocol

1. After completing Western Blot chemiluminescence detection, rinse the membrane with TBS/T or PBS/T for 5 minutes.

2. Add 10–20 mL of stripping buffer to cover the membrane. Incubate on a shaker for 10–20 minutes (duration depends on the amount of antibody bound).

3. Discard the stripping buffer and rinse the membrane with TBS/T or PBS/T for 5 minutes.

   • Optional: Apply chemiluminescence substrate to quickly verify stripping efficiency.

4. Reblock the membrane with an appropriate blocking buffer before reprobing with other antibodies.

5. The membrane can be stripped at least 10 times without significantly affecting signal intensity.

   • Example: GAPDH signals showed no significant reduction after 6 hours of continuous stripping (equivalent to 24 cycles at 15 min each).

Specifications

Specifiche e purezza

BioReagent, ready-to-use, suitable for electrophoresis, for western blot

Stabilità e conservazione

Store at 2-8℃ long term (12 months).

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Adatto all'elettroforesi, BioReagent, per il Western blot, Pronto all’uso

Immagini

Stripping Efficiency of the WB Antibody Rapid Stripping Buffer (Mild, Universal Type) (W1491287)

Stripping Efficiency of the WB Antibody Rapid Stripping Buffer (Mild, Universal Type) (W1491287) 

Experimental Method:

The same PVDF membrane was sequentially probed with antibodies against different target proteins, with antibody stripping performed using this product in between. 

Results: 

Before stripping (left): Immunoblotting with Recombinant CEP55 Antibody (Ab336940) (target molecular weight ~55 kDa) showed a clear and specific band, indicating efficient primary antibody binding. 

After stripping (right): Following a 15‑minute incubation with this product at room temperature, the same membrane was re‑probed with Recombinant Hsp70 Antibody (Ab108909) (target molecular weight ~70 kDa). A distinct, strong band for HSP70 was observed, with a clean background and no residual signal from the previous detection.