Western Blot Stripping Buffer (Acidic)

Cat. No.: W751794

GRADE & PURITY BioReagent, Ready-to-use, for Western blot

Panoramica

This product is suitable for Western Blot experiments of chemiluminescence and fluorescence detection, and can be reused on nitrocellulose or PVDF membranes that have been tested. At room temperature, primary and secondary antibodies on the membrane can be removed without affecting the protein on the membrane, without the need for repeated electrophoresis and membrane transfer, saving samples and time.
This product has a mild formula, simple and fast operation, and can perform 3-5 Western Blot tests on the same membrane.
Precautions：
1. If precipitation occurs in the solution before use, it can be heated at 37 ° C for 5 minutes to promote dissolution.
2. It is recommended to first detect the target protein with low expression levels, and then treat it with regeneration solution and detect the protein with high expression levels.
3. It is not recommended to use BSA sealed imprinting membranes, as well as DAB, NBT/BCIP stained imprinting membranes.
Instructions for Use：
1. Remove the tested membrane from the machine, clean it with PBST or TBST for 10 minutes, and discard the cleaning solution.
2. The duration of elution using regeneration solution mainly depends on the protein expression level, antibody type, and concentration. If the detection signal is not strong, it can be eluted by shaking at room temperature for 30-60 minutes; If it is a sample with high expression and strong signal, 20mM DTT or 100mM β - mercaptoethanol can be added according to its usage volume, and shaken at room temperature for 30-60 minutes for elution.
3. Discard the regeneration solution and rinse 3 times with an appropriate volume of PBST or TBST, each time for 5 minutes.
4. At this point, the antibodies on the membrane have been eluted and can be resealed for the next round of testing.

Specifications

Product Name

Western Blot Stripping Buffer (Acidic)

Sinonimi

Stripping Buffer | Stripping Solution | Western Blot Antibody Stripping Buffer

Grado

BioReagent, per il Western blot, Pronto all’uso

Specifiche e purezza

BioReagent, for western blot, ready-to-use

Tipo di molecola

Bioreagenti/Tampone

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Stabilità e conservazione

Store at room temperature long term (12 months).

Domande frequenti

What does ready-to-use mean?

ready-to-use indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.