QUALITÉ & PURETÉCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Validated, Carrier Free, ExactAb™, 1.0 mg/mL Carrier Free,ExactAb™,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.
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Stockage & expédition
Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.
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Documents qualité
SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.
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Preuve documentaire
Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.
Vue d’ensemble
Rabbit anti Human Cdc20 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
Spécifications
Product Name
Cdc20 Antibody - Primary antibody, specific to Cdc20, Rabbit IgG
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC20.
Contrôle positif
WB: A431, THP-1, U-2 OS and C6 cell lysates. IHC: Rat brain tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Méthode de purification
Immunogen affinity purified
Concentration
1.0 mg/mL
Product Line
Produits biologiques
Stockage et expédition
Forme
Liquid
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 20.0 s
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10.0 s
Cdc20 Antibody (Ab095575) - IHC Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Cdc20 Antibody (Ab095575) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Application
Application
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/1000
Documentation
📋 Fiche de données de sécurité (FDS)
Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.
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Dilution Calculator
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Reconstitution Calculator
Avis
Avis des clients
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