Donkey Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, IgG

Cat. No.: Ab136895
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 2mg/mL
Application
ELISA, IF/ICC, IHC, WB
Host species
Donkey
Reactive species
Rabbit
Isotype
Donkey IgG
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab136895-100μg
3
$57.90
1mg
Ab136895-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$302.90
500μg
Ab136895-500μg
1
$196.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 2mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Donkey Anti-Rabbit IgG H&L (Biotin), Secondary antibody, could be used for WB, ELISA, IHC, IF, ICC and so on.
Applications:
WB:1/1000-1/10000
ELISA:1/1000-1/10000
IHC :1/100-1/1000
IF/ICC:1/100-1/1000
Purity:
Affinity purified by Protein G


Conjugation notes
Biotin

Specifications

Product Name
Donkey Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, IgG
Synonyms
Donkey Anti-Rabbit IgG H&L/Biotin | Donkey Anti-Rabbit IgG | Donkey Anti Rabbit IgG | Donkey Anti Rabbit IgG (Biotin) | Donkey Anti Rabbit IgG-Biotin | Donkey Anti-Rabbit IgG-Biotin | Biotin-Donkey Anti-Rabbit IgG | Biotin- Donkey Anti-Rabbit IgG H&L | Bi
Specifications & Purity
ExactAb™, High Performance, Validated, Azide Free, 2mg/mL
Host species
Donkey
Immunogen
Native rabbit IgG
Conjugation
Biotin
Grade
Azide Free, ExactAb™, High Performance, Validated
Isotype
IgG
Purification Method
Protein G purified
Concentration
2mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Images

Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895)- ELISA
Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215) (0.5μg/mL,0.1μg/mL and uncoated control). A serial dilution of Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application
ApplicationDilution info
WB

1/1000 - 1/10000

ELISA

1/1000-1/10000

IF/ICC

1/100-1/1000

IHC

1/100-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700532Certificate of AnalysisJun 18, 2026 Ab136895
ZJ23F0700533Certificate of AnalysisJun 18, 2026 Ab136895
ZJ23F0700534Certificate of AnalysisJun 18, 2026 Ab136895
ZJ26F0636185Certificate of AnalysisJun 08, 2026 Ab136895
Documents & Articles
Antibody structure and isotypes
How to find the right secondary antibody?
Antibody applications and techniques
RNA dot blot protocol
General Protocol for Western Blotting
Guidelines of Secondary Antibody for Western Blotting
CLARITY Tissue Clearing Staining Technology
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Pathogenesis, Virological Characteristics, and Laboratory Detection Strategies for Nipah Virus (NiV)
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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