Direct eosinophil counting assay

Summary

Knowledge of eosinophil counting principles and techniques

Operation method

Direct eosinophil counting assay

Principle

With eosinophil diluent, blood is quantitatively diluted and red blood cells and most other white blood cells are destroyed to color the eosinophil rice granules, which are dropped into a counting chamber; the number of eosinophils within a certain range is counted and then calculated as the number of eosinophils per liter of blood

Materials and Instruments

Blood
Hillkelmar Ethanol-eosin dilution Eosin-acetone solution Formaldehyde Glycerol Sodium citrate
Test tubes

Move

III. Experimental reagents:
l, Hillkelmar 2, ethanol a eosin dilution solution
Eosin 0.2g φ = 0.95 ethanol 30mL
95% phenol 0.5mL Potassium carbonate 1.0g
40% formaldehyde 0.5mL Glycerol 10mL
Distilled water Add to 100ul 20g/L Eosin 10mL
Sodium citrate 0.5g
Distilled water Add to 100mL
3、Yhong a acetone liquid
20g/L Eosin 5mL
Acetone 5mL
Distilled water 90mL
IV. Experimental operation:
l, in a small test tube dilution 0.38mL.
2, take the capillary blood 20ul to join the tube after complete dissolution of red blood cells, drop into the counting cell. Standing 3-5min, with low magnification counting two counting chamber in 10 large square eosinophils number V. Calculation: eosinophils / L = 1O large square eosinophils × 20 × 106 normal reference value: 0.05-0.5 × ¹⁰⁹ / L

For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/