FGF23 Mouse mAb: Detection, Applications & Protocol Guide

FGF23 Mouse mAb is a monoclonal antibody raised in mouse designed to specifically detect Fibroblast Growth Factor 23 (FGF23), a key hormone regulator of phosphate homeostasis and vitamin D metabolism.

Product Name

FGF23 Mouse mAb

SKU

Ab348961

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Target Antigen

FGF23 (Fibroblast Growth Factor 23)

Supplier Reference

absea #KC-8422

What Is FGF23 and Why Does It Matter?

Fibroblast Growth Factor 23 (FGF23) is a hormone primarily secreted by osteocytes and osteoblasts in bone. It plays a central role in regulating phosphate homeostasis and vitamin D metabolism by acting on the kidneys to promote phosphate excretion and suppress the synthesis of the active form of vitamin D (1,25-dihydroxyvitamin D).

Dysregulation of FGF23 is associated with a range of clinically significant disorders. Elevated FGF23 levels are linked to hypophosphatemic rickets, chronic kidney disease (CKD)-mineral bone disorder, and cardiovascular complications. Conversely, FGF23 deficiency can lead to hyperphosphatemia and soft tissue calcification. Given its broad physiological impact, FGF23 is an important biomarker and therapeutic target in nephrology, endocrinology, and bone biology research.

FGF23 Mouse mAb: Product Overview

The FGF23 Mouse mAb (Aladdin SKU: Ab348961; absea reference KC-8422) is a monoclonal antibody generated in mice against FGF23. Monoclonal antibodies offer high specificity and reproducibility compared with polyclonal alternatives, making them the preferred tool for quantitative and qualitative detection of a defined epitope on the FGF23 protein.

This antibody is intended for research use only and is suitable for investigators studying phosphate regulation, renal physiology, bone metabolism, and FGF23-related pathologies. Its monoclonal nature ensures lot-to-lot consistency, which is critical for longitudinal studies and multi-site collaborations.

Recommended Applications and Protocols

Mouse monoclonal antibodies against FGF23 are commonly used in a variety of immunological and biochemical assays. Typical applications for antibodies of this class include:

• ELISA (Enzyme-Linked Immunosorbent Assay): Used for quantitative measurement of FGF23 in serum, plasma, urine, or cell culture supernatant. Pair the capture and detection antibodies that recognize distinct, non-overlapping FGF23 epitopes for sandwich ELISA formats.
• Western Blotting (WB): Detects FGF23 protein in cell lysates or tissue extracts after SDS-PAGE separation and membrane transfer. Optimize primary antibody concentration and blocking conditions for best results.
• Immunohistochemistry (IHC): Enables localization of FGF23 expression in fixed tissue sections, particularly useful for bone and kidney tissue studies.
• Immunofluorescence (IF): Suitable for visualizing FGF23 distribution in cultured cells or tissue cryosections.

For specific dilution factors, incubation times, and validated conditions, consult the product datasheet or contact Aladdin Scientific technical support. Always include appropriate positive and negative controls in each assay.

Storage, Handling, and Quality Considerations

To maintain the activity and specificity of FGF23 Mouse mAb, observe the following general best practices for monoclonal antibody handling:

• Storage Temperature: Store at -20°C for long-term preservation. Avoid repeated freeze-thaw cycles, which can degrade antibody performance. Aliquot upon receipt if repeated use is anticipated.
• Short-term Storage: For working stocks used within 1-2 weeks, storage at 4°C is typically acceptable. Do not store at room temperature for extended periods.
• Carrier Protein and Preservatives: The formulation may contain stabilizers such as BSA or glycerol; consult the datasheet for exact composition. If the antibody contains sodium azide, take appropriate precautions as it is toxic and incompatible with peroxidase-based detection systems.
• Sterility: Maintain aseptic technique when aliquoting to prevent microbial contamination.

Always verify lot-specific information on the certificate of analysis (CoA) provided with each shipment.

FGF23 Research Context: Key Biological Pathways

FGF23 exerts its biological effects primarily through binding to FGF receptor 1 (FGFR1) in complex with the co-receptor Klotho, predominantly in the kidney. Downstream signaling involves activation of the MAPK/ERK and PI3K/Akt pathways, leading to reduced expression of sodium-phosphate cotransporters (NaPi-IIa and NaPi-IIc) and suppression of CYP27B1 (the enzyme producing active vitamin D) while upregulating CYP24A1 (which degrades active vitamin D).

Understanding this pathway is essential for interpreting FGF23 immunoassay data. Researchers should be aware that FGF23 circulates in both an intact biologically active form and C-terminal fragments generated by proteolytic cleavage. Some antibodies recognize only the intact form, while others detect all fragments. Confirm whether the FGF23 Mouse mAb (Ab348961) targets an intact-specific or pan-FGF23 epitope by consulting the product datasheet, as this critically affects data interpretation in clinical and experimental settings.

Frequently asked questions

What species does FGF23 Mouse mAb (Ab348961) detect?

The antibody is raised in mouse (host species) against FGF23. For species reactivity (e.g., human, rat, mouse FGF23 antigen), please refer to the official product datasheet or contact Aladdin Scientific technical support, as this information is not confirmed in the current source material.

Which applications is FGF23 Mouse mAb validated for?

Mouse monoclonal antibodies targeting FGF23 are commonly used in ELISA, Western blotting, immunohistochemistry, and immunofluorescence assays. For the specific validated applications of Ab348961, please consult the product datasheet, as detailed validation data are not provided in the current source material.

How should FGF23 Mouse mAb be stored to maintain optimal performance?

General best practice for monoclonal antibodies is to store at -20°C for long-term preservation, avoiding repeated freeze-thaw cycles by aliquoting upon receipt. Short-term working stocks can typically be kept at 4°C for up to one to two weeks. Always confirm storage conditions on the product-specific datasheet or CoA.

Does FGF23 Mouse mAb detect intact FGF23 or C-terminal fragments?

FGF23 circulates as both an intact hormone and C-terminal fragments. Whether Ab348961 is specific to the intact form or recognizes all molecular forms (pan-FGF23) depends on the epitope targeted. Please verify this critical detail in the product datasheet before designing your experimental assay.

Can FGF23 Mouse mAb be used for sandwich ELISA?

Monoclonal antibodies are widely used in sandwich ELISA formats by pairing capture and detection antibodies that recognize distinct, non-overlapping epitopes on FGF23. Whether Ab348961 has been validated as a capture or detection antibody in a sandwich pair is not specified in the current source material; please contact Aladdin Scientific for pairing recommendations.

References

1. Shimada T, et al. Cloning and characterization of FGF23 as a causative factor of tumor-induced osteomalacia. Proceedings of the National Academy of Sciences. 2001.
2. Quarles LD. Endocrine functions of bone in mineral metabolism regulation. Journal of Clinical Investigation. 2008.
3. Wolf M. Update on fibroblast growth factor 23 in chronic kidney disease. Kidney International. 2012.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.