HNF4A Mouse mAb: FAQs for Research Use

HNF4A Mouse mAb is a mouse-derived monoclonal antibody targeting hepatocyte nuclear factor 4 alpha (HNF4A), a key transcription factor involved in liver development, metabolism, and differentiation.

SKU

Ab351242

Product Name

HNF4A Mouse mAb

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS Only

Target Protein

HNF4A (Hepatocyte Nuclear Factor 4 Alpha)

Overview of HNF4A and Its Biological Significance

HNF4A (Hepatocyte Nuclear Factor 4 Alpha) is a member of the nuclear receptor superfamily of ligand-dependent transcription factors. It plays a critical role in the regulation of gene expression in the liver, kidney, intestine, and pancreas. HNF4A governs hepatic differentiation, lipid and glucose metabolism, bile acid synthesis, and urea cycle function. Dysregulation of HNF4A has been associated with maturity-onset diabetes of the young (MODY1), hepatocellular carcinoma, and inflammatory bowel disease. As a transcription factor, HNF4A is exclusively localized in the nucleus, making nuclear staining a critical quality indicator when validating antibody performance.

Product Specifications and Formulation

The HNF4A Mouse mAb (SKU: Ab351242) is a mouse monoclonal antibody supplied in a PBS-only formulation, meaning it does not contain preservatives such as sodium azide or carrier proteins such as BSA unless otherwise specified. Researchers should be aware that preservative-free, BSA-free formulations are particularly suitable for conjugation workflows, including direct labeling with fluorophores or enzymes. Storage conditions and recommended working dilutions should be confirmed upon receipt of the product datasheet, as these specifications are subject to lot-specific validation. The source reference for this antibody is listed as absea catalog number KC-3993.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies targeting HNF4A are commonly employed in a variety of immunological and molecular biology applications. Typical applications include Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), and flow cytometry (FC). Because HNF4A is a nuclear transcription factor, antigen retrieval steps are particularly important in IHC workflows to expose the epitope adequately in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Researchers are advised to consult the specific product datasheet for Ab351242 to confirm validated applications and species reactivity before use, as these details are lot-specific and subject to change.

Storage, Handling, and Stability

Proper storage is essential to maintain antibody activity and ensure reproducible experimental results. As a general guideline for monoclonal antibodies in PBS-only formulations without preservatives: aliquot the antibody upon first use to avoid repeated freeze-thaw cycles, which can degrade antibody performance. Store aliquots at -20°C for long-term preservation. Avoid storage at 4°C for extended periods when no preservative is present, as this may lead to microbial contamination. Do not expose the antibody to repeated temperature fluctuations. Specific expiry and stability data should be confirmed by consulting the certificate of analysis (CoA) provided with each lot of Ab351242.

Ordering and Customer Support

HNF4A Mouse mAb (Ab351242) is available for order through Aladdin Scientific. To place an order, visit the Aladdin Scientific website and search by SKU Ab351242, or contact your regional sales representative. For technical questions regarding application optimization, cross-reactivity, or protocol guidance, please reach out to Aladdin Scientific's technical support team. Bulk quantities and custom formulations may be available upon request. For questions related to shipping, payment, returns, or account management, please refer to the relevant Help and Customer Service sections on the Aladdin Scientific website.

Frequently asked questions

What is HNF4A Mouse mAb and what does it target?

HNF4A Mouse mAb (Ab351242) is a mouse-derived monoclonal antibody that specifically targets HNF4A (Hepatocyte Nuclear Factor 4 Alpha), a nuclear receptor transcription factor critical for liver, kidney, intestine, and pancreatic gene regulation. It is produced from a hybridoma cell line derived by fusion of mouse myeloma cells with spleen cells from an immunized mouse.

What formulation is HNF4A Mouse mAb (Ab351242) supplied in?

Ab351242 is supplied in a PBS-only formulation, indicating it does not contain preservatives (such as sodium azide) or stabilizing proteins (such as BSA) unless specifically stated on the datasheet. This makes it particularly suitable for antibody conjugation applications. Researchers should aliquot and store at -20°C to preserve activity.

In which experimental applications can HNF4A Mouse mAb be used?

Mouse monoclonal antibodies against HNF4A are commonly used in Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), and flow cytometry (FC). Please consult the specific datasheet for Ab351242 to confirm which applications have been experimentally validated, as well as the recommended working dilutions for each application.

How should I store HNF4A Mouse mAb to maintain its activity?

Upon receipt, aliquot the antibody to minimize freeze-thaw cycles and store aliquots at -20°C for long-term use. Since the formulation is PBS-only without preservatives, avoid prolonged storage at 4°C to prevent potential microbial growth. Always refer to the Certificate of Analysis (CoA) for lot-specific stability and expiry information.

How can I order HNF4A Mouse mAb (Ab351242) from Aladdin Scientific?

You can order Ab351242 directly from the Aladdin Scientific website by searching for the SKU Ab351242, or by contacting your regional Aladdin Scientific sales representative. For bulk orders, custom formulations, or further technical assistance, please contact Aladdin Scientific customer support.

References

1. Absea Biotechnology. KC-3993 HNF4A Mouse Monoclonal Antibody product information.
2. Sladek FM. What are nuclear receptor ligands? Molecular and Cellular Endocrinology. 2011.
3. Gonzalez FJ. Regulation of hepatocyte nuclear factor 4 alpha-mediated transcription. Drug Metabolism and Pharmacokinetics. 2008.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.