p53 Mouse mAb: FAQs for Research Use

p53 Mouse mAb is a monoclonal antibody raised in mouse that targets the p53 tumor suppressor protein, enabling researchers to detect and study this critical regulator of the cell cycle and apoptosis in a variety of experimental applications.

Product Name

p53 Mouse mAb

SKU / Catalog Number

Ab350604

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation / Buffer

PBS only (per absea#KC-2397)

Target Protein

p53 tumor suppressor (TP53)

Overview of p53 and Its Role in Research

The p53 protein, encoded by the TP53 gene, is one of the most extensively studied tumor suppressor proteins in biomedical research. It functions as a transcription factor that responds to cellular stresses such as DNA damage, hypoxia, and oncogenic signaling, triggering downstream responses including cell-cycle arrest, DNA repair, senescence, and apoptosis.

Mutations in TP53 are detected in a large proportion of human cancers, making reliable detection of p53 protein expression and mutational status a cornerstone of both basic and translational cancer research. A high-quality monoclonal antibody such as p53 Mouse mAb (Ab350604) provides the specificity and lot-to-lot consistency required for rigorous experimental work.

Product Specifications and Formulation

p53 Mouse mAb is supplied under catalog number Ab350604. The antibody is formulated in PBS only (phosphate-buffered saline), without additional stabilizers such as BSA or glycerol, as referenced in internal specification KC-2397. Researchers should note this formulation when designing storage and dilution protocols.

Because the buffer contains no added carrier proteins or cryoprotectants, it is recommended to aliquot the antibody upon receipt to minimize freeze-thaw cycles, and to store working aliquots at 4 °C for short-term use. Please refer to the product datasheet for confirmed concentration, clone identity, isotype, and recommended dilutions, as these details require verification from the official product record.

Recommended Applications and Usage Guidelines

Monoclonal antibodies against p53 are widely employed across a spectrum of immunological and molecular techniques. Common applications for anti-p53 mouse monoclonal antibodies as a class include:

• Western Blotting (WB) — detection of p53 protein at its expected molecular weight (~53 kDa) in cell and tissue lysates.
• Immunohistochemistry (IHC) — localization of p53 in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
• Immunofluorescence (IF) — visualization of p53 subcellular localization.
• Immunoprecipitation (IP) — enrichment of p53 and associated protein complexes.
• Flow Cytometry (FC) — quantitative analysis of p53 expression at the single-cell level.

Note: The specific validated applications for Ab350604 should be confirmed on the official product datasheet. Not all applications listed above may be validated for this particular lot or clone.

Storage, Handling, and Stability

Proper storage and handling are essential to maintain antibody activity and ensure reproducible results:

• Short-term storage (up to 1 month): Store at 4 °C. The PBS-only formulation means the antibody lacks cryoprotectant; minimize time at room temperature.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles, which can cause aggregation and loss of binding activity.
• Handling: Centrifuge briefly before opening the vial. Work on ice where possible. Do not vortex vigorously.
• Sterility: Maintain aseptic technique if the antibody is intended for cell-based assays.

Specific shelf-life and expiry information are provided on the product label and certificate of analysis (CoA). Contact Aladdin Scientific technical support for lot-specific stability data.

Ordering, Returns, and Customer Support

p53 Mouse mAb (Ab350604) is available to order directly from Aladdin Scientific. To place an order or request a quotation, researchers and procurement staff may:

• Visit the Aladdin Scientific website and search for catalog number Ab350604.
• Contact the Aladdin Scientific sales team by email or telephone for bulk pricing, institutional agreements, or custom requirements.

Returns and Refunds: Aladdin Scientific's standard return policy applies. If the product does not meet the stated specifications upon receipt, please contact customer service within the applicable claims window and retain the original packaging and cold-chain documentation. Antibody products are typically non-returnable once the cold chain has been broken, unless a quality defect is confirmed.

Shipping: The product is shipped on dry ice or with cold packs appropriate for the formulation. Delivery timelines vary by region; contact your local Aladdin Scientific representative for estimated lead times.

For technical questions regarding experimental protocols, cross-reactivity, or troubleshooting, contact Aladdin Scientific Technical Support and reference catalog number Ab350604.

Frequently asked questions

What is the formulation or buffer of p53 Mouse mAb (Ab350604)?

p53 Mouse mAb (Ab350604) is formulated in PBS (phosphate-buffered saline) only, without added stabilizers such as BSA or glycerol. Researchers should aliquot the antibody upon receipt and minimize freeze-thaw cycles to preserve activity.

What applications is p53 Mouse mAb validated for?

Anti-p53 mouse monoclonal antibodies are commonly used in Western Blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, and Flow Cytometry. The specific validated applications for Ab350604 should be confirmed on the official product datasheet, as validation may vary by clone and lot.

How should p53 Mouse mAb be stored to maintain its activity?

For short-term use (up to one month), store at 4 °C. For long-term storage, aliquot into single-use volumes and keep at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles and do not vortex vigorously. Because the formulation is PBS only, extra care should be taken to limit freeze-thaw events.

What is the catalog number for p53 Mouse mAb at Aladdin Scientific?

The catalog number for p53 Mouse mAb at Aladdin Scientific is Ab350604. You can search this SKU directly on the Aladdin Scientific website or provide it to your procurement team when placing an order.

What is the molecular weight of p53 and why does it sometimes appear at a higher molecular weight on Western blots?

The p53 protein has a predicted molecular weight of approximately 43–44 kDa based on amino acid sequence, but it typically migrates anomalously at ~53 kDa on SDS-PAGE, which is the basis for its name. Post-translational modifications such as phosphorylation and ubiquitination can further shift its apparent molecular weight. This is a well-established textbook characteristic of the p53 protein.

References

1. Aladdin Scientific product record: p53 Mouse mAb, catalog number Ab350604, internal specification absea#KC-2397.
2. Lane D.P. (1992) p53, guardian of the genome. Nature, 358, 15–16.
3. Vogelstein B., Lane D., Levine A.J. (2000) Surfing the p53 network. Nature, 408, 307–310.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.