RMND5A Antibody: Key Facts, Uses & FAQs | Aladdin

RMND5A Antibody is a research-grade primary antibody targeting the RMND5A protein, supplied in PBS formulation and catalogue under SKU Ab350688 by Aladdin Scientific.

SKU

Ab350688

Target Protein

RMND5A (Required for Meiotic Nuclear Division Protein 5 Homolog A)

Formulation

PBS (Phosphate-Buffered Saline) only

Clone / Reference

absea#OC-297

Antibody Type

Primary antibody

Supplier

Aladdin Scientific

Overview of RMND5A and Its Biological Relevance

RMND5A (Required for Meiotic Nuclear Division Protein 5 Homolog A) is a member of the RMND5 family of proteins that contain a RING finger domain, which is characteristic of E3 ubiquitin ligases. E3 ubiquitin ligases play a central role in the ubiquitin-proteasome system (UPS) by recognizing specific substrate proteins and facilitating their ubiquitination, thereby targeting them for proteasomal degradation or modulating their activity. RMND5A has been implicated in the regulation of cell cycle progression and protein quality control. Research into RMND5A and its interaction partners is relevant to understanding fundamental cellular homeostasis mechanisms.

Product Specifications and Formulation

The Aladdin Scientific RMND5A Antibody (Ab350688) is formulated in PBS (Phosphate-Buffered Saline) without additional preservatives or carrier proteins unless otherwise specified at the time of ordering. The internal reference for this antibody is absea#OC-297. Researchers should consult the product datasheet for confirmed host species, clonality (monoclonal or polyclonal), isotype, and validated applications before experimental use. Storage conditions, recommended working dilutions, and positive control cell lines or tissue types should be verified with the accompanying documentation.

Recommended Applications and Usage Guidelines

Antibodies targeting RMND5A are commonly evaluated in applications such as Western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). The exact validated applications for Ab350688 should be confirmed on the product datasheet. When using this antibody, researchers should perform optimization experiments to determine the optimal antibody concentration, incubation time, and compatible secondary antibody. Appropriate positive and negative controls are essential for result validation. PBS-only formulations are suitable for immediate use but may require the addition of BSA or glycerol if long-term storage at 4°C is needed after first use.

Storage, Handling, and Stability

As this antibody is supplied in PBS only, proper storage is critical to maintaining antibody integrity and activity. General best practices for PBS-formulated antibodies include: short-term storage at 4°C (typically up to 1 month), and long-term storage at -20°C or -80°C in small aliquots to avoid repeated freeze-thaw cycles. Freeze-thaw cycling can denature antibody proteins and significantly reduce binding activity. Do not store at room temperature for extended periods. Upon receipt, it is recommended to centrifuge the vial briefly, aliquot into single-use volumes, and store appropriately. Refer to the product datasheet for the manufacturer's specific stability data.

Ordering and Customer Support

The RMND5A Antibody is available from Aladdin Scientific under SKU Ab350688. To place an order, visit the Aladdin Scientific website and search by SKU or product name. For bulk orders, custom formulations, or technical inquiries regarding experimental design with this antibody, contact Aladdin Scientific's technical support team. Pricing, availability, and lead times are displayed on the product page and may vary by region. For returns or quality-related concerns, refer to Aladdin Scientific's returns and refund policy available on the website.

Frequently asked questions

What is RMND5A and why is an antibody against it useful in research?

RMND5A is a RING finger domain-containing protein involved in ubiquitin-mediated protein degradation as an E3 ubiquitin ligase. An antibody against RMND5A allows researchers to detect, quantify, and study the protein's expression, localization, and interaction partners in various experimental models, contributing to the understanding of protein quality control and cell cycle regulation.

What formulation is the RMND5A Antibody (Ab350688) supplied in?

The RMND5A Antibody (Ab350688) from Aladdin Scientific is supplied in PBS (Phosphate-Buffered Saline) only, as referenced by the product description (absea#OC-297 PBS Only). Researchers requiring stabilizers such as BSA or glycerol for long-term storage should aliquot and supplement accordingly.

How should I store the RMND5A Antibody to maintain its activity?

For short-term use (up to one month), store the antibody at 4°C. For long-term storage, aliquot into single-use volumes and keep at -20°C or -80°C to prevent activity loss from repeated freeze-thaw cycles. Always consult the specific product datasheet for the manufacturer's recommended storage conditions.

Which experimental applications is Ab350688 validated for?

The specific validated applications for Ab350688 are not listed in the current source material and should be confirmed directly on the Aladdin Scientific product page or datasheet. Antibodies targeting RMND5A are generally evaluated for Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry, but application suitability must be verified for this specific lot.

How can I order the RMND5A Antibody from Aladdin Scientific?

You can order the RMND5A Antibody by searching for SKU Ab350688 on the Aladdin Scientific website and adding it to your cart. For assistance with bulk orders, institutional purchasing agreements, or technical questions, contact Aladdin Scientific's customer support or technical team directly.

References

1. Product description: RMND5A Antibody, absea#OC-297, PBS Only. Aladdin Scientific, SKU Ab350688.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.