Topic

GST-Tagged Protein Purification

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

1 resources · protocols, FAQs & articles
protocol

GST (Glutathione S-transferase) tag is a commonly used fusion tag with a molecular weight of ~26 kDa and good water solubility. It is often used to improve soluble expression of hydrophobic proteins or proteins prone to inclusion body formation. Using chromatography resins immobilized with ...

Updated Dec 2025·3 min read
Related topics

Techniques often explored alongside immunological experiments.

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