Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The UPS is the primary pathway for protein degradation within cells. It tags specific substrate proteins with ubiquitin (Ub), which are then recognized by the 26S proteasome and degraded into small peptides. This system maintains protein homeostasis and regulates processes such as the cell ...
Trypsin is a typical endopeptidase belonging to the serine protease family. Its primary catalytic mechanism involves the specific hydrolysis of peptide bonds at the carboxyl side of basic amino acids (arginine or lysine).
In situ hybridization (ISH) is an experimental technique used to detect and locate specific nucleic acid sequences (DNA or RNA) in cells or tissues. It uses labeled nucleic acid probes to specifically bind to target nucleic acid sequences, thereby observing and analyzing the expression and ...
One of the most common post-translational modifications of eukaryotic proteins is glycosylation. Glycosylation of proteins can affect many biological activities. For therapeutic glycoproteins, it can alter biological activity, targeting, transport, serum half-life, clearance and receptor ...
Techniques often explored alongside immunological experiments.
