WDR25 Antibody: FAQs, Applications & Protocol Guide

WDR25 Antibody is a research-grade immunological reagent designed to specifically detect the WDR25 (WD Repeat-Containing Protein 25) target, enabling reliable characterization of this protein across a range of standard laboratory applications.

Product Name

WDR25 Antibody

Catalog Number

Ab348723

Target Protein

WDR25 (WD Repeat-Containing Protein 25)

Protein Family

WD repeat (WDR) protein family

Recommended Storage

Store at -20°C; avoid repeated freeze-thaw cycles (standard recommendation for antibody reagents — verify with datasheet)

Supplier Reference

absea#OC-385

What Is WDR25 and Why Is It Studied?

WDR25 (WD Repeat-Containing Protein 25) is a member of the WD repeat protein superfamily, one of the largest and most functionally diverse protein families in eukaryotes. WD repeat domains typically consist of ~40-amino-acid repeating units that often terminate in tryptophan-aspartate (W-D) dipeptides, and these domains serve as scaffolds for the assembly of multiprotein complexes.

WD repeat proteins are implicated in a wide range of cellular processes, including signal transduction, cell cycle regulation, apoptosis, and vesicular trafficking. While the precise biological role of WDR25 is still under active investigation, antibodies against WDR25 serve as essential tools for researchers studying its expression patterns, subcellular localization, and potential involvement in disease-relevant pathways.

Note: Specific functional data for WDR25 is limited in the current literature; researchers are encouraged to consult the latest publications and the product datasheet for the most current characterization information.

WDR25 Antibody Applications and Compatibility

The WDR25 Antibody (Ab348723) is intended for use in standard immunodetection applications. Typical applications for antibodies of this class include:

• Western Blot (WB): Detection of WDR25 protein in cell or tissue lysates under denaturing conditions.
• Immunofluorescence (IF): Visualization of WDR25 subcellular localization in fixed cells or tissue sections.
• Immunohistochemistry (IHC): Detection of WDR25 expression in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
• Immunoprecipitation (IP): Enrichment of WDR25 protein from complex biological samples for downstream analysis.

Important: Confirmed application compatibility, species reactivity, recommended dilutions, and validated lot-specific data should always be verified against the official product datasheet available from Aladdin Scientific. The supplier reference for this product is absea#OC-385.

General Protocol Guidance for WDR25 Antibody Use

The following represents general best-practice guidelines for antibody-based detection experiments. Always refer to the specific product datasheet for validated, lot-tested conditions.

Western Blot (WB) — General Guidelines

1. Prepare cell or tissue lysates using an appropriate lysis buffer supplemented with protease inhibitors.
2. Resolve proteins by SDS-PAGE and transfer to a PVDF or nitrocellulose membrane.
3. Block membrane with 5% non-fat dry milk or BSA in TBST for 1 hour at room temperature.
4. Incubate with the WDR25 primary antibody at the recommended dilution (see datasheet) overnight at 4°C.
5. Wash with TBST (3 × 10 min) and incubate with an appropriate HRP-conjugated secondary antibody for 1 hour at room temperature.
6. Detect signal using a chemiluminescent substrate and image accordingly.

Immunofluorescence (IF) — General Guidelines

1. Fix cells with 4% paraformaldehyde for 15 minutes; permeabilize with 0.1–0.3% Triton X-100 in PBS for 10 minutes.
2. Block with 5% normal serum for 1 hour at room temperature.
3. Incubate with WDR25 primary antibody at the recommended dilution overnight at 4°C.
4. Wash and incubate with a fluorophore-conjugated secondary antibody; counterstain nuclei with DAPI if needed.
5. Mount coverslips and image by fluorescence microscopy.

Dilutions, incubation times, and buffer conditions should be optimized for each specific experimental system.

Troubleshooting Common WDR25 Antibody Issues

Researchers may occasionally encounter challenges when using the WDR25 Antibody. The following troubleshooting guidance addresses the most common issues:

Problem	Possible Cause	Suggested Solution
No signal detected	Antibody concentration too low; antigen masked; incompatible application	Titrate antibody concentration; verify antigen retrieval (for IHC/IF); confirm application compatibility on datasheet
High background	Insufficient blocking; antibody concentration too high; secondary antibody cross-reactivity	Extend blocking time; reduce primary antibody concentration; use species-specific secondary antibodies
Non-specific bands (WB)	Insufficient washing; overloaded protein sample	Increase wash stringency and duration; reduce total protein loaded per lane
Weak or variable signal	Sample degradation; suboptimal storage of antibody	Ensure protease inhibitors are used during lysis; verify antibody was stored at -20°C and not subjected to repeated freeze-thaw cycles

For persistent issues, contact Aladdin Scientific technical support, referencing catalog number Ab348723 and supplier reference absea#OC-385.

Storage, Handling, and Quality Assurance

Proper storage and handling of the WDR25 Antibody are essential for maintaining reagent integrity and ensuring reproducible experimental results.

• Recommended Storage Temperature: -20°C (long-term); 4°C (short-term working stocks, typically up to 1 month — verify with datasheet).
• Freeze-Thaw Cycles: Minimize the number of freeze-thaw cycles. Aliquoting the antibody upon receipt is strongly recommended to avoid degradation.
• Reconstitution: If supplied lyophilized, reconstitute according to the specific instructions on the product datasheet.
• Working Dilution: Prepare fresh working dilutions immediately before use in an appropriate diluent (e.g., TBST with 1% BSA).
• Lot-Specific Validation: Each lot of Ab348723 is subject to quality control testing. Refer to the certificate of analysis (CoA) for lot-specific data including confirmed reactivity and recommended dilutions.

For questions regarding storage, formulation, or quality assurance for Ab348723, contact Aladdin Scientific customer support.

Frequently asked questions

What is the catalog number for the WDR25 Antibody sold by Aladdin Scientific?

The WDR25 Antibody is available from Aladdin Scientific under catalog number Ab348723. The supplier reference for this product is absea#OC-385. Please use this catalog number when placing orders or contacting technical support.

Which species reactivity has been confirmed for the WDR25 Antibody (Ab348723)?

Specific confirmed species reactivity for Ab348723 is not detailed in the available product summary and must be verified against the official product datasheet or certificate of analysis provided by Aladdin Scientific. Common reactivities for research antibodies of this type may include human and mouse, but this should not be assumed without datasheet confirmation.

What applications is the WDR25 Antibody validated for?

Validated applications for Ab348723 should be confirmed using the official product datasheet. Generally, antibodies in this class are evaluated for Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunoprecipitation (IP), but confirmed application-specific validation data must be obtained from the datasheet or certificate of analysis.

How should I store the WDR25 Antibody to maintain its activity?

As a general best practice for research antibodies, WDR25 Antibody should be stored at -20°C for long-term preservation and at 4°C for short-term use. Repeated freeze-thaw cycles should be avoided by aliquoting the antibody upon receipt. Always confirm storage conditions with the specific product datasheet for Ab348723.

What is the function of WDR25 and why is it a research target?

WDR25 is a member of the WD repeat protein family, a large superfamily of scaffold proteins involved in mediating protein-protein interactions and assembly of multiprotein complexes. WD repeat proteins are broadly implicated in cellular processes such as signal transduction, cell cycle control, and apoptosis. The precise biological role of WDR25 is still being characterized, making it a subject of ongoing research interest.

How do I contact Aladdin Scientific if I experience problems with Ab348723?

If you experience technical issues or have questions about the WDR25 Antibody (Ab348723), please contact Aladdin Scientific's technical support or customer service team. When reaching out, please have your catalog number (Ab348723) and supplier reference (absea#OC-385) ready to facilitate prompt assistance.

References

1. Smith TF, Gaitatzes C, Saxena K, Neer EJ. The WD repeat: a common architecture for diverse functions. Trends Biochem Sci. 1999;24(5):181-185. doi:10.1016/S0968-0004(99)01384-5
2. Stirnimann CU, Petsalaki E, Russell RB, Müller CW. WD40 proteins propel cellular networks. Trends Biochem Sci. 2010;35(10):565-574. doi:10.1016/j.tibs.2010.04.008
3. Aladdin Scientific. WDR25 Antibody, Cat. No. Ab348723 [Product Datasheet]. Aladdin Scientific; [date not specified].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.