HLA E Antibody - Primary antibody, specific to HLA-E, Rabbit IgG

Cat. No.: Ab108192

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human HLA E Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Relevance
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Specifications

Product Name

HLA E Antibody - Primary antibody, specific to HLA-E, Rabbit IgG

Synonymes

EA1.2 antibody | EA2.1 antibody | HLA 6.2 antibody | HLA class I histocompatibility antigen alpha chain E antibody | HLA class I histocompatibility antigen E alpha chain precursorantibody | HLA class I histocompatibility antigen E alpha chain precursor an

Spécifications et pureté

ExactAb™, Validated, 1.0 mg/mL

Espèce hôte

Rabbit

Spécificité

HLA-E

Immunogène

A synthetic peptide derived from human HLA-E

Contrôle positif

WB: A431, Jurkat, THP-1, K562, Raji, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Immunogen affinity purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HLA E Antibody (Ab108192)

HLA E Antibody (Ab108192) - Western Blot
All lanes: HLA E Antibody (Ab108192) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 35 kDa
Exposure time: 7.0 s

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HLA E Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.