Hsp70 Mouse mAb - Primary antibody, specific to Hsp70, Mouse IgG1

Cat. No.: Ab187006

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

CD03/1D7

Conjugation

Unconjugated

Vue d’ensemble

WB: 1/1,000 - 1/100,000; Predicted molecular weight: 70.0 KDa

Spécifications

Product Name

Hsp70 Mouse mAb - Primary antibody, specific to Hsp70, Mouse IgG1

Synonymes

Epididymis secretory protein Li 103 antibody | FLJ54303 antibody | FLJ54370 antibody | FLJ54392 antibody | FLJ54408 antibody | FLJ75127 antibody | Heat shock 70 kDa protein 1 antibody | Heat shock 70 kDa | DnaK type molecular chaperone HSP70 1 antibody |

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Espèce hôte

Mouse

Spécificité

Hsp70

Immunogène

Recombinant protein corresponding to Human Hsp70

Contrôle positif

WB: HeLa, HEK293, A549 and K562 whole cell lysates

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150.0 KDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: Hsp70 Mouse mAb (Ab187006)

Hsp70 Mouse mAb (Ab187006) - Western Blot
All lanes: Hsp70 Mouse Antibody (Ab187006) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 73 kDa
Exposure time: 3.0 s

Western Blot: Hsp70 Mouse mAb (Ab187006)

Hsp70 Mouse mAb (Ab187006) - Western Blot
All lanes: Hsp70 Mouse Antibody (Ab187006) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 0.1 s

Application

Application

Dilution info

WB

1/1,000 - 1/100,000; Predicted molecular weight: 70.0 KDa

Cibles associées (humaines)

HSPA1A Tchem Heat shock 70 kDa protein 1A (0 Activities)

Discover Target: HSPA1A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSPA1A Tchem Heat shock 70 kDa protein 1 (164 Activities)

Discover Target: HSPA1A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Application Protocols

Item-specific tested applications and dilutions

• Not specified for this item; refer to CoA/Spec Sheet for validated applications, recommended dilutions, and positive control materials.

General workflow guidance (literature/general knowledge; not item-specific)

• Western blot: Start 0.1–1 µg/mL primary antibody; use heat-shocked cell lysates as positive control. Include housekeeping protein for loading control.
• Immunofluorescence/ICC: Start 1–5 µg/mL; fix with PFA or methanol per epitope accessibility; permeabilize with 0.1–0.3% Triton X-100 if intracellular.
• Immunoprecipitation: 1–5 µg antibody per mg lysate protein; use Protein A/G selection based on isotype (isotype for this item is not specified—test both A and G resins or verify via CoA).
• ELISA: Coat capture Ab or use as detection reagent per platform; optimize 0.05–1 µg/mL.

These parameters are starting points only and must be optimized by the user.

Biological Roles

This antibody targets Hsp70 (Heat Shock Protein 70) family members. The following summarizes the biology of Hsp70 (literature/general knowledge; not item-specific efficacy claims):

• Function: Hsp70s are ATP-dependent molecular chaperones that assist protein folding, prevent aggregation, and facilitate protein translocation across membranes. They cooperate with Hsp40/DnaJ co-chaperones and nucleotide exchange factors (NEFs) to cycle between ATP-bound (low-affinity) and ADP-bound (high-affinity) client states.
• Isoforms: In mammals, cytosolic inducible Hsp70 (e.g., HSPA1A/HSPA1B) and constitutive Hsc70 (HSPA8) are major paralogs; organelle-specific members include BiP/GRP78 (HSPA5, ER lumen) and mortalin/Grp75 (HSPA9, mitochondria). Antibody cross-reactivity among paralogs depends on epitope; exact cross-reactivity for this item is not specified.
• Cellular pathways: Key roles in proteostasis, unfolded protein response, stress granule dynamics, chaperone-mediated autophagy, and antigen presentation.
• Regulation: Transcriptionally upregulated by HSF1 upon heat/oxidative/proteotoxic stress; post-translational modifications (phosphorylation, acetylation) modulate activity and interactions.
• Research relevance: Biomarker of cellular stress; used to monitor heat-shock induction, proteasome inhibition responses, and chaperone-client remodeling in neurodegeneration and oncology research.

Item-specific details such as recognized isoform(s), epitope, and species cross-reactivity are not specified; consult CoA/Spec Sheet for definitive target coverage.

Buffer Applications

As an antibody reagent, this product functions within biological buffers rather than as a buffering agent itself.

• Item-specific formulation: PBS Only; Carrier Free; Azide Free (per Product Data). Exact pH/ionic strength are not specified.

• Practical buffer guidance (general, not item-specific):

  • Working buffers: PBS or TBS with 0.05–0.1% Tween-20 for immunoassays; add 1–5% BSA or non-fat dry milk for blocking where compatible. For carrier-free stocks, users may supplement with 0.1–0.5% carrier protein in working solutions if needed to reduce nonspecific adsorption.
  • Enzyme-based detection: Azide-free status avoids HRP inhibition; keep buffers free of azide when using peroxidase conjugates.
  • Storage buffers: For long-term frozen storage, many labs add 30–50% glycerol and/or sugars (e.g., 100–300 mM trehalose). Additives are user-supplied; none are included in this item by default.
  • Conjugation: Exchange into amine-free buffers (e.g., PBS without Tris) for NHS chemistry; remove imidazole, primary amines, and azide when relevant.

Note: Specific buffer recipes and stability windows for this SKU are not provided; refer to the CoA/Spec Sheet and perform small-scale pilot tests when modifying buffer composition.

Green Alternatives

Protein antibodies are inherently aqueous reagents; sustainability focuses on formulation and logistics rather than solvent substitution.

• Item-specific greener aspects:

  • Azide Free: Eliminates sodium azide, reducing acute aquatic toxicity and simplifying waste handling compared with azide-preserved antibodies.
  • PBS Only and Carrier Free: Minimal additive footprint; end-users can add only what is necessary for their workflow.
  • Shipped cold (ice chest + ice pads) rather than on dry ice, reducing CO₂ emissions relative to some frozen shipments (when stability allows).

• General greener best practices (not item-specific):

  • Right-size aliquots to minimize waste from freeze–thaw damage.
  • Use reusable cold packs and insulated shippers where validated.
  • Consolidate orders to reduce transport frequency.
  • For buffer exchange, choose gravity desalting columns over high‑solvent dialysis setups to limit solvent use.

• Tradeoffs:

  • Azide-free formulations may have reduced shelf-life at 2–8°C once opened; users may add benign stabilizers (e.g., trehalose, glycerol) post-aliquoting to balance longevity with environmental impact.
  • Maintaining cold chain consumes energy; plan deliveries to avoid reshipments and ensure immediate receipt.

Pharmaceutical Uses

Not applicable. This product is supplied strictly for research use only and is not intended for human or animal therapeutic, diagnostic, or clinical use.

• General context (not item-specific): Monoclonal antibodies may serve as analytical reagents in bioprocess development (e.g., process monitoring, release testing) but require appropriate qualification/validation before use in regulated settings.
• Pharmacopeia/compendial status for this SKU: Not specified for this item; refer to CoA/Spec Sheet if any compliance information is available.

Physical Properties

Item-specific physical data

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, formulation details beyond “PBS Only”: Not specified for this item; refer to CoA/Spec Sheet.
• pH of formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Additives: Carrier Free, Azide Free (per Product Data); otherwise not specified.

Inapplicable classical properties for proteins/antibodies

• Boiling point, melting point, refractive index, logP: Not applicable to macromolecular protein reagents.

General/literature values (not item-specific)

• Typical immunoglobulin (IgG) molecular mass: ~150 kDa (literature), but actual mass varies with isotype and glycosylation; specific MW for this antibody is not specified.
• Solubility: Monoclonal antibodies are water-soluble in physiological buffers (e.g., PBS) and denature in most organic solvents; avoid strong chaotropes unless for analytical purposes.
• Stability: Antibodies are temperature-sensitive; maintain cold chain and avoid freeze–thaw to preserve binding activity (see Storage & Reconstitution for item-specific conditions).

Quality and Grades

Item-specific grade descriptors (from Product Data)

• Carrier Free: No protein stabilizers (e.g., BSA, gelatin) are added. Advantages: precise protein:antibody ratios in conjugations and reduced background in certain assays. Consider adding your own stabilizer post-aliquoting if long-term storage requires it (see Storage & Reconstitution).
• Azide Free: No sodium azide preservative. Benefits: compatible with live-cell applications and peroxidase-based detection; reduced hazardous waste considerations.
• PBS Only: Formulated solely in phosphate-buffered saline (exact pH/ionic strength not specified for this item; refer to CoA/Spec Sheet).
• Validated; High Performance; ExactAb™: Marketing/quality indicators signifying that the antibody has undergone internal performance qualification. Specific validation datasets, clone ID, and isotype are not specified for this item; consult CoA/Spec Sheet.

What these grades imply for use (general guidance)

• Analytical consistency: Monoclonals typically offer lot-to-lot epitope fidelity; verify with provided CoA for your lot.
• Conjugation readiness: Carrier-free, azide-free PBS facilitates direct labeling (e.g., NHS-ester dyes, enzymes), but confirm buffer pH and absence of amine contaminants prior to conjugation.
• Documentation: For regulated workflows, request certificate(s) detailing host species, isotype, concentration, endotoxin/bioburden (if applicable); not specified for this item.

Reaction and Applications

As an antibody reagent, Hsp70 Mouse mAb is used in bioanalytical and cell/molecular biology workflows rather than chemical synthesis.

• Item-specific applications: Not specified for this item; refer to CoA/Spec Sheet.

• General research applications for anti-Hsp70 antibodies (literature/general knowledge, not item-specific claims):

  • Western blotting (WB) to detect inducible Hsp70 expression following heat shock or proteotoxic stress.
  • Immunofluorescence (IF) or immunocytochemistry (ICC) to visualize subcellular localization of Hsp70 in cytosol and stress granules.
  • Immunoprecipitation (IP)/co-IP to enrich Hsp70 complexes and probe chaperone–client interactions.
  • Flow cytometry (permeabilized cells) to quantify intracellular Hsp70 levels.
  • ELISA or bead-based assays to measure Hsp70 in cell lysates or extracellular vesicle preparations.

• Practical tips (general):

  • Use protease and phosphatase inhibitors during lysis; Hsp70 can be abundant yet dynamically regulated.
  • For stress-induction studies, validate induction conditions (e.g., 42–45°C heat shock, proteasome inhibition) and sampling time-courses.
  • Include loading and isotype controls; titer the antibody to maximize signal-to-noise.

Note: The above applications are general possibilities for anti-Hsp70 antibodies and are not verified for this specific SKU without the manufacturer’s application data.

Reaction Conditions

Not applicable to chemical reactions in the classical sense. Antibody use involves assay conditions rather than synthetic reaction conditions.

• General assay condition guidance (not item-specific):
  • Temperature: Keep on ice during setup; conduct incubations at 4°C (overnight) or room temperature (1–2 h) depending on assay.
  • Buffers: PBS/TBS with appropriate blocking agents and detergents.
  • Concentration/titration: Determine optimal working dilution empirically via checkerboard titration; starting ranges for IgG primaries often span 0.05–2 µg/mL for WB and 0.5–10 µg/mL for IF/ICC. These are general literature ranges, not specifications for this item.

Item-specific tested conditions are not provided; consult CoA/Spec Sheet.

Safety and Handling

GHS/SDS information

• Signal word, hazard statements, pictograms, and classification: Not specified for this item; refer to the product SDS for authoritative safety data.

Item-specific notes

• Formulation: PBS Only; Azide Free; Carrier Free. The absence of sodium azide reduces risks of azide-related toxicity and heavy-metal azide formation in plumbing.
• Shipping: Ice chest + ice pads (cold chain maintained).
• Storage: Store at -20°C; avoid repeated freezing and thawing (see Storage & Reconstitution).

General precautions for antibody reagents (good laboratory practice; not item-specific)

• PPE: Wear lab coat, gloves, and safety glasses. Avoid inhalation/ingestion and contact with skin/eyes.
• Handling: Keep on ice during use. Aliquot promptly after first thaw to minimize freeze–thaw cycles which can promote aggregation and loss of affinity.
• Incompatibilities: Avoid strong oxidizers, extremes of pH, proteases, and organic solvents (which may denature proteins). Do not mix with azide-containing buffers if downstream peroxidase-based detection is planned.
• First aid: In case of contact, rinse with water. If inhaled/ingested, seek medical attention per SDS guidance.
• Waste: Dispose of proteinaceous solutions according to institutional biological/chemical waste protocols; azide-free formulation simplifies disposal compared with azide-containing buffers.

Solvent Selection

This product is an aqueous protein reagent rather than a small-molecule solute. Classical solvent selection is not typically applicable.

• Item-specific medium: PBS Only (per Product Data). No additional stabilizers or azide present.
• Practical guidance (general):
  • Maintain in aqueous physiological buffers (PBS, TBS). Avoid organic solvents (acetone, acetonitrile, methanol, DMSO) which can denature or precipitate IgG.
  • For conjugation chemistries requiring specific pH or additives (e.g., NHS-ester labeling at pH 8.3), buffer-exchange into amine-free buffers as needed (e.g., PBS adjusted with bicarbonate). Use desalting columns or centrifugal filters.
  • For live-cell or enzyme-based applications, azide-free formulation is already compatible.
• If viscosity/surfactant considerations arise, low levels of nonionic detergents (e.g., 0.01% Tween-20) may be added by the user for assay performance; none are included in this item per Product Data.

Storage and Reconstitution

Item-specific storage (from Product Data)

• Storage conditions: Store at -20°C.
• Handling: Avoid repeated freezing and thawing.
• Formulation notes: PBS Only; Carrier Free; Azide Free.
• Appearance and physical state: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance (general; align with item-specific notes)

• Upon receipt: Verify container integrity; keep cold. If supplied frozen, thaw on ice.
• Aliquoting: Immediately divide into single-use aliquots to minimize freeze–thaw cycles. Use low-protein-binding tubes.
• Optional stabilizers (user-added, not included in this item): 10–50% glycerol and/or 100–300 mM trehalose can improve freeze–thaw resilience at -20°C; confirm compatibility with downstream assays.
• Short-term storage: For azide-free preparations, limit 2–8°C storage to brief periods (hours to a few days) unless a preservative is added by the user.
• Do not expose to repeated temperature cycling or vigorous agitation to prevent aggregation.

Reconstitution

• If supplied lyophilized: Reconstitution details (diluent, target concentration) are not specified for this item; refer to CoA/Spec Sheet. In general, reconstitute with sterile PBS (matching original buffer) and allow gentle agitation on ice.
• If supplied in liquid PBS: Use directly; perform buffer exchange where your application requires specific conditions (e.g., conjugation chemistry).

Always consult the lot-specific CoA/SDS for definitive handling instructions.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Hsp70 Mouse mAb (SKU: Ab187006)
• Type: Mouse monoclonal antibody against Hsp70
• Grade/Purity descriptors: Carrier Free; ExactAb™; Azide Free; Validated; High Performance; PBS Only
• CAS, CID, InChIKey, SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General antibody structure (literature/general knowledge, not item-specific)

• Monoclonal antibodies are Y‑shaped immunoglobulins comprising two heavy chains and two light chains stabilized by disulfide bonds.
• Typical domains include variable regions (VH/VL) forming the antigen-binding site (paratope) and constant regions (CH/CL) mediating effector functions.
• The antigen-binding site recognizes a defined epitope on the target protein (here, Hsp70 family proteins), but exact epitope/clone for this item is not specified.

2D structural description (general)

• IgG schematic features two Fab arms (each with one light chain and part of a heavy chain) and one Fc stem (heavy-chain constant domains). Glycosylation sites may be present on Fc (species- and isotype-dependent). Exact isotype for this item is not specified.

Synthetic Utility

Not applicable to chemical synthesis. This product is a mouse monoclonal antibody reagent targeting Hsp70 and is used in bioanalytical assays rather than as a synthetic building block or reagent.

• For researchers seeking chemical tools: Consider small-molecule chaperone modulators or Hsp70 ATPase probes for mechanistic studies; such tools are outside the scope of this product.

Target Specificity

Item-specific (from Product Data)

• Target name: Hsp70 (Heat Shock Protein 70)
• Host: Mouse monoclonal antibody
• Clone, isotype, epitope, species reactivity, cross-reactivity, and isoform specificity: Not specified for this item; refer to CoA/Spec Sheet.

No additional specificity data (e.g., sequence region recognized) is provided in the Product Data.

Foire aux questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp70 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.