Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG

Cat. No.: Ab100782

GRADE & PURITY Recombinant, Validated, ExactAb™, KD Validation, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG

Synonymes

Dmel\CG6376 antibody | Dmel_CG6376 antibody | drosE2F1 antibody | E(Sev-CycE)3A antibody | E(var)3-93E antibody | E2-promoter binding facto antibody | E2F 1 antibody | E2F transcription factor 1 antibody | E2F-1 antibody | E2f-PA antibody | E2f-PB antibod

Spécifications et pureté

Recombinant, ExactAb™, KD Validation, Validated, See COA

Mécanismes biochimiques et physiologiques

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF

Espèce hôte

Rabbit

Spécificité

E2F1

Immunogène

A synthetic peptide derived from human E2F1 (AA 365-400)

Contrôle positif

WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, KD Validation, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 66, 74 kDa
Exposure time: 3.8 s

Western Blot: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).

IHC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IHC
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).

Application

• Application: Dilution info
• IHC:

  1/100 - 1/200;

• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/50 - 1/200;

Cibles associées (humaines)

E2F1 Tbio Transcription factor E2F1 (0 Activities)

Discover Target: E2F1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

E2F1 Tbio Transcription factor E2F1 (14 Activities)

Discover Target: E2F1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.