Bleu trypan - ≥60% , CAS No.72-57-1

CAS: 72-57-1 Cat. No.: T108743 Formule: C₃₄H₂₄N₆Na₄O₁₄S₄ Poids moléculaire: 960.81 Beilstein Registry Number: 4360496 Numéro CE: 200-786-7

GRADE & PURITY ≥60%

Synonyms

Centraline Blue 3B | Bleue diretto 3B | Modr Prima 14 [Czech] | Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate | Benzanil Blue R | Congoblau 3B | Dianilblau H3G | TRYPAN BLUE [MART.] | Diaminblau 3B | NCGC00167554-01 | TRYPAN BLUE [VANDF] | Bl

Storage

Desiccated,Room temperature

Shipped In

Normal

Vue d’ensemble

Le bleu Trypan est un colorant acide bleu tétrasulfoné, hydrophile et azoïque, largement utilisé pour évaluer la viabilité des cellules. Méthode facile pour déterminer la viabilité des cellules, le bleu Trypan colore en bleu les cellules mortes dont les membranes sont perméables, alors que le colorant est exclu par la plupart des cellules vivantes et leurs membranes intactes, ce qui permet de déterminer visuellement les cellules vivantes par rapport aux cellules mortes. Bien que le bleu Trypan soit principalement utilisé pour évaluer la viabilité des cellules cultivées, d'autres applications de ce colorant ont été rapportées. Dans des tranches d'organes abdominaux de rats, le bleu Trypan a été utilisé pour déterminer la viabilité des cellules ainsi que la viabilité des amas de cellules d'îlots de Langerhans à partir d'échantillons de pancréas de caprins.
Un colorant de viabilité pour quantifier les cellules vivantes par rapport aux cellules mortes

Specifications

Synonymes

Centraline Blue 3B | Bleue diretto 3B | Modr Prima 14 [Czech] | Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate | Benzanil Blue R | Congoblau 3B | Dianilblau H3G | TRYPAN BLUE [MART.] | Diaminblau 3B | NCGC00167554-01 | TRYPAN BLUE [VANDF] | Bl

Spécifications et pureté

≥60%

Conditions de stockage de stockage

Desiccated,Room temperature

Expédié en

Normal

Pureté

≥60%

Noms et identifiants

Pubchem Sid

508813676

Sourires canoniques

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]

IUPAC Name

tetrasodium;5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate

InChIKey

GLNADSQYFUSGOU-UHFFFAOYSA-J

INCHI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

Isomères SMILES

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]

WGK Allemagne

3

RTECS

QJ6475000

Poids moléculaire

960.81

Beilstein

4360496

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Classe Benzene and substituted derivatives
      • Subclass Biphenyls and derivatives
        • Level5 Benzidines
          • Level6 3,3'-disubstituted benzidines

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Classe: Benzene and substituted derivatives
• Subclass: Biphenyls and derivatives
• Intermediate Tree Nodes: Benzidines
• Direct Parent: 3,3'-disubstituted benzidines
• Alternative Parents: 2-naphthalene sulfonic acids and derivatives 2-naphthalene sulfonates 1-sulfo,2-unsubstituted aromatic compounds Toluenes Phenoxides Sulfonyls Organosulfonic acids Azo compounds Propargyl-type 1,3-dipolar organic compounds Primary amines Organooxygen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives Organic cations
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: 3,3'-disubstituted benzidine - 2-naphthalene sulfonate - Naphthalene sulfonic acid or derivatives - 2-naphthalene sulfonic acid or derivatives - Naphthalene sulfonate - Naphthalene - Arylsulfonic acid or derivatives - 1-sulfo,2-unsubstituted aromatic compound - Phenoxide - Toluene - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Azo compound - Organic alkali metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic salt - Organic sodium salt - Amine - Primary amine - Hydrocarbon derivative - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic oxygen compound - Organic nitrogen compound - Organic cation - Aromatic homopolycyclic compound
• Description: This compound belongs to the class of organic compounds known as 3,3'-disubstituted benzidines. These are organic compounds containing a benzidine skeleton, which is substituted only at the 3- and 3'-positions.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propriétés chimiques et physiques

• Solubilité: Soluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
• Point de fusion (°C): >300°C
• Poids moléculaire: 960.800 g/mol
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 20
• Rotatable Bond Count: 5
• Exact Mass: 959.982 Da
• Monoisotopic Mass: 959.982 Da
• Topological Polar Surface Area: 404.000 Ų
• Heavy Atom Count: 62
• Formal Charge: 0
• Complexity: 1790.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 5

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