CD14 Mouse mAb - 一次抗体, specific to CD14

Cat. No.: Ab093874

GRADE & PURITY ExactAb™, Validated, See COA

Application

Flow Cytometry, WB

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

OTI4H4

Conjugation

Unconjugated

概要

Mouse anti Human CD14 Antibody, Monoclonal (OTI4H4), could be used for WB, Flow Cytometry and so on.

Specifications

Product Name

CD14 Mouse mAb - 一次抗体, specific to CD14

同義語

CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod

仕様と純度

ExactAb™, Validated, See COA

生化学的・生理学的メカニズム

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec

宿主種

マウス

特異性

CD14

免疫原

Recombinant protein of human CD14 (AA 20-367)

ポジティブコントロール

WB: HL60 and A549 cell lysates. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY CD14.

活用

Unconjugated

等級

ExactAb™, Validated

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: CD14 Mouse mAb (Ab093874)

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 15.0 s

Western Blot: CD14 Mouse mAb (Ab093874)

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 7.8 s

Flow Cytometry: CD14 Mouse mAb (Ab093874)

CD14 Mouse mAb (Ab093874) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by CD14 Mouse mAb (Ab093874), and then analyzed by flow cytometry (1/100).

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

Flow Cytometry

1/100

WB

1/500 - 1/1000

関連ターゲット（人間）

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD14 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.