CD14 マウス mAb (PE) - 一次抗体, specific to CD14

Cat. No.: Ab210091

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:575nm, 5 μL/test

Application

Flow Cytometry

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG2a

Clone Number

UCHM-1

Conjugation

PE

概要

マウス抗ヒト CD14 抗体, モノクローナル (UCHM-1) は、フロー、WB、IHC、IF、ICC、IP などに使用できます。

Specifications

Product Name

CD14 マウス mAb (PE) - 一次抗体, specific to CD14

同義語

CD14抗原｜CD14分子｜CD14｜単球分化抗原CD14｜骨髄球特異的ロイシンリッチ糖タンパク質｜CD14抗体｜CD_antigen=CD14抗体｜CD14抗体｜CD14抗原抗体｜CD14分子抗体｜CD14_HUMAN抗体

仕様と純度

ExactAb™、検証済み、Ex:565nm、Em:575nm、5μL/test

生化学的・生理学的メカニズム

MD-2およびTLR4と協力して、細菌のリポ多糖（LPS）に対する自然免疫応答を仲介する。MyD88、TIRAP、TRAF6を介して作用し、NF-κB活性化、サイトカイン分泌、炎症反応を引き起こす。細胞表面分子をアップレギュレートする

宿主種

マウス

特異性

CD14

免疫原

Human Thymocytes followed by peripheral blood mononuclear cells

ポジティブコントロール

Flow: Human peripheral blood monocytes

活用

PE

励起波長(nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

発光波長(nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

等級

Em:575nm, Ex:565nm, ExactAb™, Validated

ライトチェーン・タイプ

kappa

精製方法

Protein G purified

集中

5 μL/test

保管と配送

保管条件

2-8℃で保存、遮光、冷凍禁止

入荷

濡れた氷

安定性と保管

抗体溶液は原液のまま2℃～8℃で保存し、長時間の光照射を避けてください。凍結しないでください。

画像

Flow Cytometry: CD14 Mouse mAb (PE) (Ab210091)

CD14 Mouse mAb (PE) (Ab210091) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD14 Mouse mAb (PE) (Ab210091) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE) (Ab180308).

Flow Cytometry: CD14 Mouse mAb (PE) (Ab210091)

CD14 Mouse mAb (PE) (Ab210091) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD14 Mouse mAb (PE) (Ab210091) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• Flow Cytometry:

  5 μL/test, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood ;

関連ターゲット（人間）

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD14 Mouse mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.