CD3 epsilon Mouse mAb - 一次抗体, specific to CD3E, Mouse IgG1

Cat. No.: Ab094446

グレード＆純度 Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IHC, WB

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

C10

Conjugation

Unconjugated

概要

Mouse anti Human CD3 epsilon Antibody, Monoclonal (C10), could be used for WB, IHC and so on.
Application:
WB: 0.01-3µg/mL
IHC: 5-20µg/mL
Protein Function:
The CD3 complex mediates signal transduction.

仕様

Product Name

CD3 epsilon Mouse mAb - 一次抗体, specific to CD3E, Mouse IgG1

同義語

T cell surface antigen T3/Leu 4 epsilon chain antibody | T cell surface glycoprotein CD3 epsilon chain antibody | T-cell surface antigen T3/Leu-4 epsilon chain antibody | T-cell surface glycoprotein CD3 epsilon chain antibody | T3E antibody | TCRE antibod

仕様と純度

Carrier Free, ExactAb™, Validated, High Performance, See COA

宿主種

マウス

特異性

CD3E

免疫原

Recombinant CD3e expressed in E.coli (AA 28-185)

ポジティブコントロール

WB: Jurkat and MOLT-4 cell lysates. IHC: Human spleen tissue.

活用

Unconjugated

等級

Carrier Free, ExactAb™, High Performance, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A + Protein G purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: CD3 epsilon Mouse mAb (Ab094446)

CD3 epsilon Mouse mAb (Ab094446) - Western Blot
All lanes: CD3 epsilon Mouse mAb (Ab094446) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 22 kDa
Exposure time: 20.0 s

IHC: CD3 epsilon Mouse mAb (Ab094446)

CD3 epsilon Mouse mAb (Ab094446) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL CD3 epsilon Mouse mAb (Ab094446)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

0.01-3 µg/mL

IHC

5-20 µg/mL

関連ターゲット（人間）

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 epsilon Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.