CD3 Mouse mAb (AF488) - 一次抗体, specific to CD3, Mouse IgG1

Cat. No.: Ab189661
注文可能
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:490nm ? Fluorescence excitation maximum at 490 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:525nm ? Fluorescence emission maximum at 525 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 5 μL/test
Application
Flow Cytometry
Host species
マウス
Reactive species
人間
Isotype
Mouse IgG1
Clone Number
UCHT1
Conjugation
AF488
★
Size
USA
ドイツ (EU)*
Price
Qty
10T
Ab189661-10T
在庫あり ≥10
—
$81.90
25T
Ab189661-25T
8 在庫あり
—
$163.90
100T
Ab189661-100T
5 在庫あり
—
$409.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test Em:525nm,Ex:490nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

概要

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

Specifications

Product Name
CD3 Mouse mAb (AF488) - 一次抗体, specific to CD3, Mouse IgG1
同義語
CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a
仕様と純度
ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test
生化学的・生理学的メカニズム
The CD3 complex mediates signal transduction.
宿主種
マウス
特異性
CD3
免疫原
Human CD3ε
ポジティブコントロール
Flow: Human peripheral blood lymphocytes
活用
AF488
励起波長(nm)
Blue Laser(488nm)
発光波長(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
等級
Em:525nm, Ex:490nm, ExactAb™, Validated
アイソタイプ
Mouse IgG1
ライトチェーン・タイプ
kappa
精製方法
Protein G purified
集中
5 μL/test
保管と配送
保管条件
Store at 2-8°C,Protected from light,Do not freeze
入荷
Wet ice,Do not freeze
安定性と保管
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
画像

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245).Black - Unlabelled control, cells without incubation with primary antibody.

適用#テキヨウ#
適用#テキヨウ#Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

関連ターゲット(人間)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
作用メカニズム
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type日付商品
ZJ24F0910463Certificate of AnalysisApr 07, 2026 Ab189661
ZJ24F0910464Certificate of AnalysisApr 07, 2026 Ab189661
ZJ24F0910465Certificate of AnalysisApr 07, 2026 Ab189661
よくある質問と記事
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Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:490nm grade guide → View Em:525nm grade guide →

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